Isolation and molecular identification of hydrocarbon degrading bacteria from oil-contaminated soil

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Research Paper 01/10/2017
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Isolation and molecular identification of hydrocarbon degrading bacteria from oil-contaminated soil

Fatiha Dilmi, Abdelwaheb Chibani, Khadidja Senouci Rezkallah
Int. J. Biosci.11( 4), 272-283, October 2017.
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The spills of hydrocarbon due to the petrochemical industry are major contaminants in the environment. Bioremediation is an effective, economical and environmentally sound treatment. The purpose of our study was to isolate, screen and identified the hydrocarbon degrading bacteria from oil polluted soil. Three oil contaminated soil were collected from Arzew oil refinery, North-west of Algeria. Sixteen bacterial strains were isolated using mineral salt media supplemented with 1% of crude oil; these isolates were screened for their best degradation abilities. Four selected bacterial strains designated as (P2.3, P2.2, S15.1 and E1.1) were identified on the basis of morphological, biochemical and molecular characterization using 16S rRNA gene sequence analysis. The sequences were compared to the closest relative species in the GenBank database of National Centre for Biotechnology Information. The growths rates of the selected isolates were determined using spectrophotometer at 600nm.Based on the partial 16S rRNA gene sequencing and phylogenetic analysis; the isolates were identified as Pseudomonas aeruginosa P2.3, Achromobacter xylosoxidans P2.2, Staphylococcus haemolyticus S15.1 and Enterococcus faecalis E1.1. Results indicated that the isolates strains had effectively utilize crude oil as sole carbon source. Linear increase in Optical Density (OD) was observed between days 4 and 10. Pseudomonas aeruginosa P2.3 and Achromobacter xylosoxidans P2.2 showed the highest growth in media with crude oil. This study indicates that the contaminated soil samples contain a diverse population of hydrocarbon degrading bacteria and these strains could be used for the bioremediation of oil contaminated soil.


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