Isolation, Characterization and Control of Bacteria from Failed Root Canal Treatment

Paper Details

Research Paper 20/08/2022
Views (1075)
current_issue_feature_image
publication_file

Isolation, Characterization and Control of Bacteria from Failed Root Canal Treatment

Shakila Sharmin, Farzana Ashrafi Neela
Int. J. Biosci. 21(2), 378-383, August 2022.
Copyright Statement: Copyright 2022; The Author(s).
License: CC BY-NC 4.0

Abstract

This cross-sectional type of descriptive study was carried out to isolates, characterization and control of bacteria from failed root canal treatment. The sample size was 101 which was selected purposively. Partially structured questionnaire was used to collect data from the respondents by face to face interview. All efforts were made to collect data accurately. For open questions, the respondents were asked in such a manner way so that they could speak freely and explain their opinion in a normal and neutral way. In total 101 patients 72 were male and 29 were female and the age group between 11-70 years. The first resistance antibiotic is Cephradine and second resistance Cefuroxime. The first sensitive antibiotic is Amoxyclav and second sensitive Cefuroxime. Total 101 patient Streptococcus spp. found Male 43, Female 54 Staphylococcus spp. Male 23, Female 8 and Enterococcus spp. Male 6, Female 10. The most commonly found bacteria is Streptococcus spp. So this study would help the physician to select the proper antibiotics for RCT and make a new era to control the failure of RCT.

Gomes B, Herrera DR. 2018. Etiologic role of root canal infection in apical periodontitis and its relationship with clinical symptomatology. Brazilian Oral Research 32(suppl 1), 69.

Li X, Zhu XF, Zhang CF, Cathro P, Seneviratne CJ, Shen S. 2013. Endodontic bacteria from primary and persistent endodontic lesions in Chinese patients as identified by cloning and 16S ribosomal DNA gene sequencing. Chinese Medical Journal (Engl).  126, 634–639.

Marsh PD. 2009. Are dental diseases examples of ecological catastrophes?. Microbiology 149, 279–294.

Molander A, Reit C, Dahlén G, Kvist T. 1998. Microbiological status of root-filled teeth with apical periodontitis. International Endodontic Journal 31, 1–7.

Murad CF, Sassone LM, Faveri M, Hirata R, Jr, Figueiredo L, Feres M. 2014. Microbial diversity in persistent root canal infections investigated by checkerboard DNA-DNA hybridization. Journal of Endodontics 40, 899–906.

Narayanan LL, Vaishnavi C. 2010. Endodontic microbiology. Journal of Conservative Dentistry 13, 233–9.

Rôças IN, Siqueira JF, Jr. 2010. Identification of bacteria enduring endodontic treatment procedures by a combined reverse transcriptase–polymerase chain reaction and reverse-capture checkerboard approach. Journal of Endodontics 36, 45–52.

Rôças IN, Siqueira JF, Jr. 2012. Characterization of microbiota of root canal-treated teeth with posttreatment disease. Journal of Clinical Microbiology 50, 1721–1724.

Siqueira J. 2001. Aetiology of root canal treatment failure: why well-treated teeth can fail. International Endodontic Journal 34, 1–10.

Siqueira J, Rocas I, Lopes H, Uzeda M. 1999. Coronal leackage of two root canal sealers containing calcium hydroxide after exposure to human saliva. Journal of Endodontics 25, 14–6.

Tabassum S, Khan FR. 2016. Failure of endodontic treatment: The usual suspects. European Journal of Dentistry 10, 144–7.

Tennert C, Fuhrmann M, Wittmer A, Karygianni L, Altenburger MJ, Pelz K. 2014. New bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings. Journal of Endodontics 40, 670–677.

Zargar N, Marashi MA, Ashraf H, Hakopian R, Beigi P. 2019. Identification of microorganisms in persistent/secondary endodontic infections with respect to clinical and radiographic findings: bacterial culture and molecular detection. Iranian Journal of Microbiology 1, 120-128.

Related Articles

The protective effect of black pepper (Piper nigrum) on liver enzymes in streptozotocin-induced diabetic rats

Amani A. R. Filimban, Khulud A. Wathi, Int. J. Biosci. 27(4), 140-149, October 2025.

Production of bioplastics (PHB) using waste paper as feed stock by Cupriavidus taiwanensis

Ajeena Davis, Jini Joseph, Int. J. Biosci. 27(4), 130-139, October 2025.

Gill ectoparasites of the mugilidae from the Ebrié lagoon, Abidjan (Côte d’Ivoire)

Eby Yoboué Gnamma Honorine Alla, Carel Wilfried Bermian Dibi-Ahui, Fidèle Kouassi Kouakou, Abouo Béatrice Adepo-Gourene, Int. J. Biosci. 27(4), 123-129, October 2025.

Impacts of diverse water management systems on growth and yield of two prominent boro rice cultivars in Bangladesh

Zahidul Islam, Md Ekhlasur Rahman, Md Khayrul Islam Bashar, Sharmin Sultana, Md Taharat Al Tauhid, Md Rabiul Islam, Md Shahed Hossain, Md Musa Mondal, Pradip Kumar Biswas, Int. J. Biosci. 27(4), 110-122, October 2025.

First record of Brachymeria excarinata Gahan, 1925 (Hymenoptera: Chalcididae) parasitizing Plutella xylostella (L.) (Lepidoptera: Plutellidae) in west Africa

Babacar Labou, Etienne Tendeng, El hadji Sérigne Sylla, Mamadou Diatte, Karamoko Diarra, Int. J. Biosci. 27(4), 104-109, October 2025.

Assessment of adsorption isotherms of three plantain flours (Musa paradisiaca L. var. Horn 1, FHIA 21 and PITA 3) and cassava flour (Manihot esculenta Crantz var. Bonoua 2)

Brou Koffi Siméon, Yue Bi Yao Clément, Kane Fako, Douali Gohi Bi Douali Jean-Sory, Tano Kablan, Int. J. Biosci. 27(4), 93-103, October 2025.

Exploring the antioxidant efficacy of boldine: A natural compound with broad-spectrum activity

Maharani Jaganathan, Kathiresan Suresh, Manickam John, Rajeswari Vasu, Theerthu Azhamuthu, Nihal Ahamed Abulkalam Asath, Ravichandran Pugazhendhi, Pratheeba Veerapandiyan, Int. J. Biosci. 27(4), 82-92, October 2025.

Assessment of genetic parameters and yield trait stability in sweet sorghum genotypes through AMMI and GGE biplot approaches

A. H. Inuwa, H. A. Ajeigbe, Y. Mustapha, B. S. Aliyu, I. I. Angarawai, Int. J. Biosci. 27(4), 69-81, October 2025.