Isolation, identification and antibiotic susceptibility pattern of Salmonella species isolated from poultry samples of District Kohat

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Research Paper 01/01/2019
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Isolation, identification and antibiotic susceptibility pattern of Salmonella species isolated from poultry samples of District Kohat

Inam Ullah, Nasir Mahmood Khattak, Zahidullah Khan, Hazir Rehman, Hassan Naveed, Malik Nawaz Shuja, Muhammad Naeem, Matiullah, Ikram Ullah, Hameed Ur Rehman
Int. J. Biosci. 14(1), 284-292, January 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

Salmonella species has been recognized as a major cause of food borne illness associated with poultry worldwide. Salmonellosis is one of the most common food borne diseases in industrial and developing countries. The wide spread of antimicrobial-resistance in Salmonella species has been a serious global human and animal health problem. The aims of this study were to estimate the prevalence and antimicrobial susceptibility pattern of Salmonella species from poultry samples of District Kohat. The isolates were screened for antimicrobial susceptibility pattern by using disk diffusion method. Out of 100 poultry samples, 35 were positive for Salmonella species. Most Salmonella species were resistant to Ampicillin (n=28; 80%) and Tetracycline (n=27; 78%).40% (n=14) were susceptible to Azithromycin, 31.42% (n=11) were intermediate and 28.57% (n=10) were resistant to Azithromycin 37.14%. Chloramphenicol showed good antibacterial activity against Salmonella species. 18 isolates (51.42%) were sensitive to Chloramphenicol, while 37.14% (n=13) were resistant. Ciprofloxacin and Levofloxacin exhibited poor activity against Salmonella species and most of them were intermediate sensitive to Ciprofloxacin (n=18; 51.52%) and Levofloxacin (n=19; 54.28%).High levels of resistance to antibiotics that are used commonly for human and poultry can be a warning for our community health and this information must be used to form important strategies for improvement of infection control.

Shah H, Korejo NA. 2012. Antimicrobial Resistance Profile of Salmonella Serovars Isolated from Chicken Meat. Journal of Veterinary and Animal Science 2, 40-46.

Althouse CC, Patterson S, Fedorka-Cray P, Isaacson RE. 2003. Type 1 fimbriae of Salmonella enterica serovar Typhimurium bind to enterocytes and contribute to colonization of swine in vivo, Infectious Immunology 71, 6446.

CLSI (Clinical and Laboratory Standards Institute). 2012. Performance for antimicrobial disk susceptibility tests”, “approved standard, CLSI document M02-A11 ed.11th, Wayne (PA), USA: Clinical and Laboratory Standards Institute 32(1), 1–76.

CDC, the Centers for Disease Control and Prevention. 2010. National Antimicrobial Resistance Monitoring System for enteric bacteria (NARMS), “Human isolates final report, 2008”, G. A. Atlanta, The Centers for Disease Control and Prevention.

Scherer CA, Miller SI. 2001. Molecular pathogenesis of Salmonella, “Principles of bacterial pathogenesis” (E. A. Groisman), Academic Press, United States of America 265-316.

Scherer CA, Miller SI. 2001. Molecular pathogenesis of Salmonella, Principles of bacterial pathogenesis (E. A. Groisman), Academic Press, United States of America, 265-316.

Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL. 2008. GenBank”, Nucleic Acids Research 36, 25-30.

Newell DG, Koopmans M, Verhoef L, Duizer Aidara E, Kane A, Sprong H, Giessen VD, Kruse HJ. 2010. Food-borne diseases-the challenges of 20 years ago still persist while new ones continue to emerge, International Journal of Food Microbiology 139, S3-S15.

Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL. 2008. GenBank, Nucleic Acids Research 36, 25-30.

Wasyl D, Hoszowski A. 2012. First isolation of ESBL-producing Salmonella and emergence of multiresistant Salmonella Kentucky in turkey in Poland, Food Research International 45, 958-961.

Iossifidou EG, Abrahim A, Soultos ND, Triantafillou EA, Koidis PA. 2012. Antimicrobial resistance profiles in Salmonella spp. and Escherichia coli isolates from turkey samples in Northern Greece, Annals Microbiology 62, 623-628.

Duguid JP, Anderson ES, Campbell I. 1966 Fimbriae and adhesive properties in Salmonella, Journal of Pathology and Bacteriology 92, 107.

Cox JM, Pavic A. 2010. Advances in enteropathogen control in poultry production”, Journal of Applied Microbiology 108, 745-755.

Aoust JYD. 1989. Salmonella, Food-Borne Bacterial Pathogens (M. P. Doyle), Marcel Dekkar, New York 327-445.

Vera JG, Fedorka-Cray PJ. 2007. Prevalence, distribution and characterization of ceftiofur resistance in Salmonella enterica isolated from poultry form in the USA from 1999 to 2003, International Journal of Antimicrobial Agents 30(2), 134-42.

Todar K. 2005. Salmonella and salmonellosis, Todar Online Textbook of Bacteriology, Department Of Bacteriology, University of Wisconsin – Madison.

Glenn LM. Lindsey RL, Frank JF, Meinersmann RJ, Englen MD, Paula J. 2011. Analysis of antimicrobial resistance genes detected in multidrug resistant Salmonella enterica serovar Typhimurium isolated from food animals, Microbial Drug Resistance 17, 407-418.

Thomson NR, Clayton DJ, Windhorst D, Vernikos G. 2008. Comparative genome analysis of Salmonella Enteritidis PT4 and Salmonella Gallinarum 287/91 provides insights into evolutionary and host adaptation pathways, Genome Research 18, 1624.

Depoorter P, Persoons D, Uyttendaele M, Butaye P, De Zutter L, Dierick K. 2012. Assessment of human exposure to 3rd generation cephalosporin resistant Salmonella (CREC) through consumption of broiler meat in Belgium, International Journal of Food Microbiology 159, 30-38.

Krieg RN, Holt JG. 1984. Bergey’s manual of systemic Bacteriology” USA, Williams and Wilkins Company, Baltimore 308 – 429.

Parveen S, Taabodi M, Schwarz JG, Oscar TP, Harter-Dennis J, White DG. 2007. Prevalence and antimicrobial resistance of Salmonella recovered from processed poultry, Journal of Food Protection 70, 2466-2472.

Arroyo S, Yadav AS, Singh SM, Bharti P. 2010. Prevalence of Salmonella in chicken eggs collected from poultry farms and marketing channels and their antimicrobial resistance, Food Research International 43, 2027-2030.

Chia TWR, Goulter RM, McMeekin T, Dykes G,  A, Fegan N. 2009. Attachment of different Salmonella serovars to materials commonly used in a poultry processing plant, Food Microbiology 26, 853-859.

Porwollik S, McClell M. 2003. Lateral gene  transfer in Salmonella, Microbial Infection 5, 977.

Singh S, Agarwal RK, Tiwari SC, Singh H. 2012. Antibiotic resistance pattern among the Salmonella isolated from human, animal and meat in India”, Tropical Animal Health and Production 44, 665.

Lorian V. 1986. Antibiotics in laboratory medicine ed. 2nd, Williams & Wilkins, Baltimore.

Chuang Y, Wang CKC, Chen YT, Yang CH, Men SC, Fan CC, Chang LH, Yeh KS. 2008.  Identification of the genetic determinants of Salmonellaenterica serotype Typhimurium that may regulate the expression of the type 1 fimbriae in response to solid agar and static broth culture conditions, BMC. Microbiology 8, 126.

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