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Isolation, identification and screening of phosphate solubilizing bacteria from the Piper betle fields of Guntur District, Andhra Pradesh, India

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Research Paper 07/06/2024
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Isolation, identification and screening of phosphate solubilizing bacteria from the Piper betle fields of Guntur District, Andhra Pradesh, India

M. Roja Susmitha, K. Ammani
J. Bio. Env. Sci.24( 6), 19-24, June 2024.
Certificate: JBES 2024 [Generate Certificate]
Key: Phosphate solubilizationPiper betlePseudomonas fluorescens ASN-2Rhizosphere soil

Abstract

A study was designed to screen the effective phosphate solubilizing rhizobacteria from rhizosphere of Betle vine plants (Piper betle). Soil samples collected from agriculture fields of Piper betle of Guntur district (Nutakki, Revendrapadu), Andhra Pradesh, India and they were employed for isolation of rhizobacteria by using serial dilution plate technique. To isolate rhizobacteria, two media such as Nutrient agar and Kings’ B agar were employed. Total 15 rhizobacterial strains were isolated from the rhizosphere samples and designated them as ASN-1 to ASN-15 and evaluated for their phosphate solubilizing ability. Among them, ASN-2 showed phosphate solubilisation efficiency. The maximum phosphate solubilization halo zone was recorded as 30 mm on Pikovskaya’s agar plates after 96h of incubation at 28±2⁰C whereas in case of broth, the strain exhibited highest tricalcium phosphate (TCP) solubilization as 197.32μg/ml. Characterization of the strain was performed based on cultural, morphological and genomic characteristics using 16s r RNA sequencing and identified as Pseudomonas fluorescens ASN-2 (GenBank accession number: MW537707). The study suggested that rhizobacteria (Pseudomonas) from rhizosphere of vegetable crop Piper betel of Andhra Pradesh (Nutakki, Revendrapadu) serve as a source for potential phosphate solubilizing property.

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