Isolation of biofloc-associated bacteria from the rearing water of whiteleg shrimp, Penaeus vannamei in nursery tanks

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Research Paper 09/02/2023
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Isolation of biofloc-associated bacteria from the rearing water of whiteleg shrimp, Penaeus vannamei in nursery tanks

Ermae M. Liprado, Belle Grace T. Vargas, Angelica B. Villarosa, Eva Grace B. Chavez, Christopher Marlowe A. Caipang
Int. J. Biosci.22( 2), 183-191, February 2023.
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Abstract

We aimed to determine the water quality of the biofloc-based nursery tank system and to isolate and characterize biofloc-associated bacteria from the rearing water of the whiteleg shrimp, Penaeus vannamei, during the nursery production phase in response to recommendations to further study biofloc composition. Serially-diluted biofloc water samples from a shrimp nursery tank were spread onto Tryptic Soy Agar (TSA) plates, incubated for approximately 48 h at 28℃ and observed for bacterial growth. Bacterial colonies that exhibited varying morphology were re-streaked onto fresh TSA plates to obtain pure colonies. Bacterial isolates were subjected to morphological tests following standard methods. Molecular characterization of the bacterial isolates was done by amplification and sequencing of the 16S rRNA. Water quality parameters including dissolved oxygen, pH, salinity, ammonia-N, nitrite-N and nitrate-N, were monitored twice daily using commercially available kits. The isolated bacterial colonies showed variations in the margin but were all similar in opacity. The colors of the bacterial colonies ranged from light yellow to white. Staphylococcus, Pseudomonas, and a putative Bacillus velezensis from the rearing water of a biofloc-based nursery tank of whiteleg shrimp were identified. During the one-month nursery production phase, levels of the nitrogenous wastes (total ammonia-N, nitrite and nitrate) were within acceptable levels required for shrimp farming.

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