Isolation of culturable, extended spectrum beta lactamase producing Escherichia coli from fish and fish waste from local fish market

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Research Paper 08/08/2023
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Isolation of culturable, extended spectrum beta lactamase producing Escherichia coli from fish and fish waste from local fish market

Roopa Niroli, Vandana Rathod, Narmada Talloli, Dattu Singh, Ravi Talikote, Sujata Hosmani, Krishna Rayudu, Shajji Dawood
Int. J. Biosci. 23(2), 234-239, August 2023.
Copyright Statement: Copyright 2023; The Author(s).
License: CC BY-NC 4.0

Abstract

Faecal indicator patterns of antibiotic susceptibility of Escherichia coli was investigated in fresh fish purchased at retail market. The phenotypic characteristics of antibiotic resistance were investigated in 430 fresh seafood samples of ESBL E. coli (fresh fish, fish waste, and associated environment). Sixty ESBL-positive E. coli were recovered from them. A significant number of isolates were resistant to amoxicillin (98.4%), cefepime (91.9%), and both cefepime and ceftazidime (91.1%). Colistin (100%) was shown to have a relatively higher susceptibility than cefotaxime-clavulanic acid (85.5%) or amoxyclav. Over three antibiotics were resistant to all 60 isolates. The EC01 and EC59 isolates were resistant to 14 different antibiotics. Cefotaxime had MIC values of 1024g/ml for 87% of the isolates. Twenty isolates tested positive for resistance to multienzyme MICTM strips.

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