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Isolation of lactic acid bacteria from chicken gut and its probiotic potential characterization

Research Paper | September 1, 2017

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Gulmakai Shakoor, Ali Akbar, Abdul Samad, Shabir Ahmad Khan, Fazal Ur Rehman, Malalai Shakoor, Hafsa sunniya, Deedar Ahmad, AdeelRafique

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Int. J. Biosci.11( 3), 1-9, September 2017

DOI: http://dx.doi.org/10.12692/ijb/11.3.1-9


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Probiotics are now best known to be viable microorganisms that plays important role in promoting health by improving the intestinal microbial balance and inhibiting the growth of pathogenic bacterial strains. The aim of the present study was to isolate and select Lactic acid bacteria (LAB) with excellent probiotic potential and antagonism against important pathogenic bacteria. Lactic acid bacteria with bacteriocin producing ability were isolated and identified from the gastrointestinal tract (LAB) of chicken. Total of 11 strains were isolated, isolate were characterized morphologically and identification was done through different biochemical tests. For determination of antibacterial activities agar well diffusion method was used. Growth at different percentage concentration of NaCl, bile salt and resistance to various pH were all tested in broth medium. Antibiotic susceptibility was also carried out. All 11 strains showed inhibitory activities against pathogenic bacteria Klebseilla pneumonia, Staphylococcus aurous, Escherichia coli and Salmonella typhi. Isolates with code GG,SIYS with inhibition zone of (22mm,23mm)and (21mm,23mm)against Staphylococci aureus and Escherichiacoli showed best inhibitory activity. All isolated strains were revealed to be tolerant to bile salt at concentration of 3%, showed growth at all percentage concentration of NaCl and survived in both acidic and basic pH but only strain LIC failed to tolerate pH2.Probiotic characterization of the isolated bacterial strains was determined by observing its growth in various pH range (2, 8, 10), bile salt (0.2, 1, 2 and 3.0 %), temperature (25, 37, 45) and NaCl (2, 4, 6 and 8 %). Antibiotic sensitivity pattern showed that the LAB isolates were highly sensitive to Amikacin, Clarithromycin, Amikacin and Oflaxacin but were moderately sensitive to Cefotaxime, Azithromycin, but were resistant to Ampicillin and Tetracycline. The selected LAB were found to exhibits outstanding probiotic potential and can be used as a source of probiotic in human, animal and also as a natural preservative for food.


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Isolation of lactic acid bacteria from chicken gut and its probiotic potential characterization

Akbar A, Ali I, Anal AK. 2016. Industrial perspectives of lactic acid bacteria for biopreservation and food safety (review). Journal of Animal and Plant Sciences 26(4), 938-948.

Ahmed T, Kanwal R. 2004. Biochemical characteristics of lactic acid producing bacteria and preparation of camel milk cheese by using starter culture. Pakistan Veterinary Journal 24, 87-91. http://dx.doi.org/10.7324/JAPS.2013.30317

Begley M, Gahan CGM, Hill C. 2005. The interaction between bacteria and bile. Federation of European Microbiological Societies Microbiology Reviews 29, 625-651. http://dx.doi.org/10.1016/j.femsre.2004.09.003

Duke GE. 1977.  Avian digestion.  In:  G.E.  Duke, (ed.):  Physiology of Domestic Animals. 9th edition. Ithaca and London, Cornell University Press 313-320.

Elizete DFRP, Carlos RS. 2005. Biochemical characterization and identification of probiotic Lactobacillus for swine. Boletim Centro de Pesquisa de Processamento de Alimentos, 23, 299-310. http://dx.doi.org/10.5897/AJMR2015.7750

Eczema C. 2013. Probiotics in animal production.  Journal of Veterinary Medicine and Animal Health 5(11), 308-316. http://dx.doi.org/105897/JVMAH2013.0201.

Fooks LJ, Fuller R, Gibson GR. 1999. Prebiotics, probiotics and human gut microbiology.  International Dairy Journal 9, 53-61. http://dx.doi.org/10.7324/JAPS.2013.30317

Gilliland SE, Nelson CR, Maxwell CR.  1985. Assimilation of cholesterol by Lactobacillus acidophilus. Applied and Environmental Microbiology 49, 377-381. http://dx.doi.org/10.1111/1471-0307.12217

Gilliland SE, Staey TE, Bush LJ. 1984. Importance of bile tolerance of Lactobacillus acidophilus used as dietary adjunct. Journal Dairy Science, Champaign 67, 30-45.

Graciela F, De V, Maria PT. 2001. Food Microbiology Protocols.  (J.F.T.  Spencer and A.L.R.D. Spencer, Ed.), Totowa, New Jersey: Humana Press Inc., chapter. 21 2004. (Probiotic Properties of Lactobacilli) 173-181.

Karimi TMA, Rahimi SH, Mojgani N, Esmaeilkhanian S, Grimes JL. 2008. Screening of indigenousstrains of lactic acid bacteria for development of a probioticfor poultry.  Asian–Australian. Journal of Animal. Sciences 21(10), 1495 – 1500.

Kim GB, Lee BH. 2005. Biochemical and molecular insights into bile salt hydrolase in the gastrointestinal micro flora – A review. Asian-Australian Journal of Animal Sciences. 18, 1505-1515.

Khunajakr N, Wongwicharn A, Moonmangmee D, Tantipaiboonvut S. 2008. Screening and identification of lactic acid bacteria producing antimicrobial compounds frompig gastrointestinal tracts. King Mongkut’s Institute of Technology Ladkrabang. Science and Technology Journal 8, 8-17.

Nelson G, George S. 1995. Comparison of media for selection and enumeration of mouse fecal flora populations.  Journal of Microbial Methods 22, 293-300. http://dx.doi.org/10.1016/0167-7012(95)00004-5

Ouwehand AC, Kirjavainen PV, Shortt C, Salminen S. 1999. Probiotics mechanisms and established effects. International Dairy Journal9, 43-52. http://dx.doi.org/10.1016/S0958-6946(99)00043-6

Rojo-Bezares B, Sáenz Y, Poeta P, Zarazaga M, Ruiz-Larrea F, Torres C. 2006. Assessment of antibiotic susceptibility within lactic acid bacteria strains isolated from wine. International Journal of Food Microbiology 111, 234–240. http://dx.doi.org/10.1016/j.ijfoodmicro.2006.06.007

Stiles ME. 1996.  Biopreservation by lactic acid bacteria.  Antonie Van Leeuwenhoek 70, 331-45.

Savadago A,  Ouattara  CAT, Bassole IHN, Traore SA. 2006. Bacteriocins and lactic acid bacteria–a minireview. African journal of biotechnology 5(9), 678-683. http://dx.doi.org/10.7324/JAPS.2013.30317.

Shida K, Nanno M. 2008. Probiotics and immunology: separating the wheat from chaff. Trends in Immunology 29, 565-573. http://dx.doi.org/10.1016/j.it.2008.07.011

Todoro SD, Rachman C, Fourrier A, Dicks LMT, Reenen CA, Prévost H, Dousset X. 2011. Characterization of a bacteriocin produced by Lactobacillus sakei R1333 isolated from smoked salmon. Anaerobe 17, 23–31. http://dx.doi.org/10.1016/j.anaerobe.2010.01.004

Thoesen JC.  1994. Suggested procedures for the detection and identification of certain finfish and shellfish pathogens. 4th edition. Version 1, fish Health Section, American Fisheries Society.