International network for natural sciences – research journal
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Effects of soil moisture, phosphorus and zinc on isoenzymes activity and banding patterns of peroxidase in potato plant

By: Mahdi Mirashzadeh, Mostafa Valizadeh, Rahim Motalebifard, Majid Noruzi, Mahdi Taghizadegan, Mozhgan Shirinpour, ShahinUstan

Key Words: Electrophoresis, Peroxidaseisozymes, Phosphorus, Water deficit, Zinc.

J. Bio. Env. Sci. 8(1), 34-42, January 2016.

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Water deficit stress is a major abiotic factor that limits crop production. Hence plant Nutrition can have play-determining role in moderating the adverse effects of water deficit stress. This research was conducted as a factorial experiment based on randomized complete blocks design with zinc (Zn) at three levels (0, 10 and 20 mg Zn per kg dries soil as ZnSO4•7H2O), phosphorus (P) at three levels (0, 30 and 60 mg P per kg dry soil as Calcium (Ca) (H2PO4)2•H2O) and soil moisture at three levels (0.5FC-0.6FC, 0.7FC-0.8FC and 0.9FC-FC) using three replications under greenhouse conditions. The results showed that the moderate (0.7FC-0.8FC) and severe water deficit conditions (0.5FC-0.6FC) decreased significantly activity of peroxidase isozymes (POX) than to the enzyme activity in full irrigated (0.9FC-FC) conditions (P< 0.01).The higher activity of peroxidase isozymes appeared in POX1 under the moderate water deficit condition and the lowest related to POX5 isozyme under severe water deficit condition. In addition, the main effect of Zn and two way interaction of Zn × soil moisture were significant on the enzymatic activity of POX2, POX3 and POX4 isozymes. The highest activity of peroxidase isozymes resulted for POX2 at application of10 mg Zn per kg of soil. The two ways interaction of soil moisture × Zn for POX3, POX2 and POX4 showed that the effect of Zn application on these esozymes were significant only under severe water deficit condition the highest activity of POX2 and POX3 were obtained at application of 10 mg Zn per kg dried soil and for POX4 under using of 20 mg Zn per kg soil condition.

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