home icon user icon
Welcome to International Network for Natural Sciences | INNS Pub.
Home My Account
Submit Manuscript
double_slash
breadcumb_bg

Screening and characterization of amylase producing bacteria from soil contaminated with cassava effluents from Abraka, Delta State

Paper Details

Research Paper 01/02/2022
Views (1347)
current_issue_feature_image
publication_file

Screening and characterization of amylase producing bacteria from soil contaminated with cassava effluents from Abraka, Delta State

Adomi O Patience
J. Biodiv. & Environ. Sci. 20(2), 42-47, February 2022.
Copyright Statement: Copyright 2022; The Author(s).
License: CC BY-NC 4.0

Abstract

Amylases are starch hydrolyzing enzymes important in normal daily life and industry. There are different sources of amylase production however, enzymes derived from microorganisms are cheaper and thermos table among other features. The aim of this work was to isolate amylase producing bacteria from soil contaminated with cassava water effluents and to find out environmental/ optimization of amylase production. Soil samples were collected from four different sites. A total of 50 bacterial were isolated form soil samples, 14 showed area of clear zones on starch medium. Isolates that gave maximum yield of amylase were identified molecularly. The isolates were optimized for carbon sources, pH, nitrogen sources, incubation time, agro waste and chlorides. Biochemical and molecular analysis showed that Citrobacter sp and Enterobacter cloaca showed higher amylase activity. Glucose, nitrate, pH 7.0, magnesium chloride and temperature of 25oC produced maximum amylase. Plantain peel was the agro water that produced more amylase enzyme.

Certificate Cover PDF

Bole S, Maji A, Dey A, Acharya A, Dubey, Rakeshlal S. 2013. Isolation, purification and characterization of amylase fron airborne bacteria World Journal of Pharmacy& Pharmceutical Sciences 2(5), 228-246.  www.wippo.com

Ekka A, Namdeo N. 2018 Screening, Isolation and Characeterization of amylase production bacteria and optimization for production od amylase IOSR Journal of Biotechnology and Biochemistry 4(2), 50-56.

Fasiku SA, Ogunsola OF, Fakunle A, Olanbiwoninu AA. 2020. Isolation of Bacteria with potential of producing extracellular enyzmes (Amylase, cellulose and protease) from soil. Journal of Advances in Microbiology 21-26.

Fossi BT, Taveand F, Ndjonenkeu T. 2005. Production and partial characterization of a thermostable amylase from ascomycetes yeast strain isolated from starchy soils. African Journal of Biotechnology 4(1), 14-18.

Geesha. 2016. https://pediaa.com/difference-between -alpha-and-beta-amylase/ accessed 2nd November 2021.

Gupta A, Gupta VK, Modi DR, Yadawa LP. 2008. Production and characterization of amylase for Aspergillus niger. Biotechnology 7(3), 551-556.

Oluwa damilare LA, Oluwatofunmi OE, Dzorbenya AG, Farinn AO. 2019. A review of literature on isolation of bacteria alpha amylase, International Research Journal of Engineering and Technology (IRJET) 6(1), 1333-1342.

Orji JC, Nweke CO, Nwabueze RN, Nwanyanwu EN, Alisi CS, Etim-Osowo EN. 2009. Production and properties of a-amylase from Citrobacter species. Revista Ambiente &Agua. An Interdisciplinary journal of Applied Scien 4(1), 45-56.

Oseni AO. Ekperigin MM. 2013. Isolation and activity of alpha amylase from selected bacteria strains in the forest soil. GJOB 2(1), 12-20.

Pokhrel B, Wanjare P, Singh S, Purushotham B. 2013. “Isolation, screening and characterization of promising-amylase producing bacteria from sewage enriched soil”, IJABR (4), 286-290.

Saini R, Saini HS, Dahiya A. 2017. Amylases: Characterization and Industrial application Journal of Pharmacognosy and Phytochemistry 6(4), 1865-1871.

Sexena R, Singh R. 2011. Amylase production by solid state fermentation of Agro-Industrial waste using Bacillus species BTM 42, 1334-1342.

Swetha S, Gangadharon D. 2006 A- amylase from Microbial Sources- An Overview recent development Food Technol Biotechnol 44(2), 173-184.

Swetha Sivaramakrishnan, Gangadharon Dhanya. 2006. α- Amylases from Microbial Sources–An Overview on Recent Developments. Food Technol Biotechnol. March 44(2), 173-184.

Validya S, Rathore P. 2015. Isolation, screening and characterization of amylase producing bacteria for soil of potato dump sites from different regions of Madhya Pradish http://www.researchgate.net /publication /277242517

Veerapagu M, Yeeya KR, Sankaranarayaran A. 2016. Screening and production of fungal amylase from Aspergillus sp by SSF. Journal of Global Biosciences 5(8), 4443-4450.

Verma V, Avasthi S, Singh M. 2011. Amylase production and purification from bacteria isolated from waste potato dumpsite. European Journal of Experimental Biology 1, 107-113.

Related Articles

Land use conflicts: An impediment to improved agrifood value chain management as perceived by crop farmers in southeast Nigeria

J. U. Chikaire, C. C. Ejiogu, H. I. Duruanyim, T. O. Ogbuji, S. I. Ogbaa, A. O. Kalu, J. I. Ukpabi, A. Rufai, L. C. Izunobi, J. U. Okwudili, C. I. Anah, E. U. Omeire, I. O. Okeoma, J. Nnametu, U. G. Chris-Ejiogu, I. E. Edom, C. N. Atoma, U. S. Awhareno, E. C. Mube-Williams, S. O. Adejoh, A. D. Ude, J. O. Oparaojiaku, C. O. Osuagwu, E. E. Ihem, B. N. Aririguzo, E. C. C. Amaechi, M. N. Osuji, C. A. Acholonu, J. Biodiv. & Environ. Sci. 28(1), 102-113, January 2026.

Assessment of physico-chemical, heavy metal, and microbiological quality of spring water sources in Jasaan, Misamis Oriental, Philippines

Ronnie L. Besagas*, J. Biodiv. & Environ. Sci. 28(1), 96-101, January 2026.

Farmland and geospatial resource survey for food security promotion among farm families in Imo state, Nigeria

J. U. Chikaire, C. C. Ejiogu, M. N. Osuji, C. A. Acholonu, I. E. Edom, U. S. Awhareno, E. C. Mube-Williams, C. N. Atoma, S. O. Adejoh, H. I. Duruanyim, T. O. Ogbuji, S. I. Ogbaa, A. O. Kalu, J. I. Ukpabi, A. Rufai, L. C. Izunobi, J. U. Okwudili, E. U. Omeire, I. O. Okeoma, J. Nnametu, A. D. Ude, C. I. Anah, J. O. Oparaojiaku, C. O. Osuagwu, E. E. Ihem, B. N. Aririguzo, E. C. C. Amaechi, J. Biodiv. & Environ. Sci. 28(1), 78-95, January 2026.

Phytochemical composition, miticidal and pediculicidal efficacy of ethanolic leaf extracts of neem (Azadirachta indica) and tobacco (Nicotiana tabacum) against Pterolichus obtusus and Goniodes dissimilis

Roel T. Calagui*, Sherwin L. Alota, Jhaysel G. Rumbaoa, Glydel Joy T. Ragutero, Kyrone D. Ancheta, Lovely Grace V. Jacinto, Kjelle Cristlea P. Cabang, Bryan Jerome R. Bassig, J. Biodiv. & Environ. Sci. 28(1), 68-77, January 2026.

Mangroves under pressure: Local threats and management realities in Malamawi Island, Basilan, Philippines

Norvie Semine*, Jill Ruby Parmisana, Ashikeen Tampipi, Chris Rey Lituanas, Wella Tatil, J. Biodiv. & Environ. Sci. 28(1), 56-67, January 2026.

Institutional e-waste management: A knowledge, attitude, and perception study among the administrative staff at Mindanao State University, Iligan Institute of Technology, Philippines

Rezanne Mabyl Burlado*, Rodolfo II Romarate, Peter Suson, Wella Tatil, J. Biodiv. & Environ. Sci. 28(1), 40-55, January 2026.

Biomass and carbon stocks of fine litterfall and coarse woody debris in riparian and non-riparian tropical forests of Carmen, Bohol, Philippines

Carl Anthony G. Budiongan, Jairyl B. Oclarit*, Noel T. Lomosbog, J. Biodiv. & Environ. Sci. 28(1), 24-39, January 2026.

Microhabitat and seasonal influences on terrestrial mollusc communities in a reforested secondary forest, south-eastern Côte d’Ivoire

Amani N’dri Saint-Clair*, Pokou Konan Pacome, N'dri Kouassi Jerome, Otchoumou Atcho, J. Biodiv. & Environ. Sci. 28(1), 12-23, January 2026.

Submit Manuscript