Lipid peroxidation and antioxidant status in 2,4,6-octatrienoic acid treated A549 and HCT-116 cancer cells

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Research Paper 15/07/2025
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Lipid peroxidation and antioxidant status in 2,4,6-octatrienoic acid treated A549 and HCT-116 cancer cells

Shanmugam M. Sivasankaran, Raju Kowsalya, Krishnan Baskaran, Chakravarthy Elanchezhiyan
Int. J. Biosci. 27(1), 291-296, July 2025.
Copyright Statement: Copyright 2025; The Author(s).
License: CC BY-NC 4.0

Abstract

The thiobarbituric acid reactive substances (TBARS) and antioxidants status have been utilized as putative biomarkers to assess the in vitro antiproliferative effect of phytoconstituents and medicinal plants in cancer cells. This study analysed the status of lipid peroxidation and antioxidant status in untreated and octatrienoic acid treated A549 lung and HCT-116 colorectal cancer cells. The status of lipid peroxidation by-products [TBARS, Conjugated Dienes (CD) and lipid hydroperoxides] and activities of antioxidants [Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx) and Reduced glutathione (GSH)] were analysed using colorimetric assays. While octatrienoic acid increased the TBARS, CD and lipid hydrperoxides levels, it lowered the activities of SOD, CAT, GPx and GSH content in A549 and HCT-116 cells. The results of present study revealed that octatrienoic acid might have suppressed the proliferation of A549 lung and HCT-116 colorectal cancer cells through modulating the oxidative stress biomarkers. The present study concludes that octatrienoic acid facilitates the generation of lipid peroxidation by-products by reducing antioxidant defence mechanism in A549 and HCT-116 cancer cells.

Bahrami H, Tafrihi M. 2023. Global trends of cancer: The role of diet, lifestyle and environmental factors. Cancer Innov. 2, 290–301.

Barrera G. 2012. Oxidative stress and lipid peroxidation products in cancer progression and therapy. ISRN Oncology 2012, 137289.

Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. 2012. Oxidative stress and antioxidant defense. World Allergy Organ J. 5, 9–19.

Bray F, Laversanne M, Sung H, Ferlay J, Siegel RL, Soerjomataram I, Jemal A. 2024. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians 74, 229–263.

Crespo R, Rodenak-Kladniew BE, Castro MA, Soberón MV, Lavarías SML. 2020. Induction of oxidative stress as a possible mechanism by which geraniol affects the proliferation of human A549 and HepG2 tumor cells. Chemico-Biological Interactions 320, 109029.

Donnan SK. 1950. The thiobarbituric acid test applied to tissues from rats treated in various ways. Journal of Biological Chemistry 182, 415–420.

Ellman GL. 1959. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics 82, 70–77.

Flori E, Mastrofrancesco A, Kovacs D, Ramot Y, Briganti S, Bellei B, Paus R, Picardo M. 2011. 2,4,6-Octatrienoic acid is a novel promoter of melanogenesis and antioxidant defence in normal human melanocytes via PPAR-γ activation. Pigment Cell & Melanoma Research 24, 618–630.

Gonzalez MJ. 1992. Lipid peroxidation and tumor growth: An inverse relationship. Medical Hypotheses 38, 106–110.

Jiang ZY, Hunt JV, Wolff SP. 1992. Ferrous ion oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low density lipoprotein. Analytical Biochemistry 202, 384–389.

Kachadourian R, Day BJ. 2006. Flavonoid-induced glutathione depletion: Potential implications for cancer treatment. Free Radical Biology & Medicine 41, 65–76.

Kakkar P, Das B, Viswanathan PN. 1984. A modified spectrophotometric assay of superoxide dismutase. Indian Journal of Biochemistry and Biophysics 21, 130–132.

Nakamura H, Takada K. 2021. Reactive oxygen species in cancer: Current findings and future directions. Cancer Science 112, 3945–3952.

National Center for Biotechnology Information. 2025. PubChem compound summary for CID 5368831, Octa-2,4,6-trienoic acid. Retrieved June 17, 2025 from https://pubchem.ncbi.nlm.nih.gov/compound/Octa-2_4_6-trienoic-acid.

Popovici V, Bucur L, Vochita G, Gherghel D, Mihai CT, Rambu D, Calcan SI, Costache T, Cucolea IE, Matei E, Badea FC, Caraiane A, Badea V. 2021. In vitro anticancer activity and oxidative stress biomarkers status determined by Usnea barbata (L.) F.H. Wigg. dry extracts. Antioxidants (Basel) 10, 1141.

Ramírez CF, Cavieres LA, Sanhueza C, Vallejos V, Gómez-Espinoza O, Bravo LA, Sáez PL. 2024. Ecophysiology of Antarctic vascular plants: An update on the extreme environment resistance mechanisms and their importance in facing climate change. Plants (Basel) 13, 449.

Recknagel RO, Glende EA Jr. 1984. Spectrophotometric detection of lipid conjugated dienes. Methods in Enzymology 105, 331–337.

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. 1973. Selenium: Biochemical role as a component of glutathione peroxidase. Science 179, 588–590.

Sharifi-Rad M, Anil Kumar NV, Zucca P, Varoni EM, Dini L, Panzarini E, Rajkovic J, Tsouh Fokou PV, Azzini E, Peluso I, Prakash Mishra A, Nigam M, El Rayess Y, Beyrouthy ME, Polito L, Iriti M, Martins N, Martorell M, Docea AO, Setzer WN, Sharifi-Rad J. 2020. Lifestyle, oxidative stress and antioxidants: Back and forth in the pathophysiology of chronic diseases. Frontiers in Physiology 11, 694.

Sinha AK. 1972. Colorimetric assay of catalase. Analytical Biochemistry 47, 389–394.

Su P, Veeraraghavan VP, Krishna Mohan S, Lu W. 2019. A ginger derivative, zingerone—a phenolic compound—induces ROS-mediated apoptosis in colon cancer cells (HCT-116). Journal of Biochemical and Molecular Toxicology 33, e22403.

Yue L, Li Y, Luo Y, Alarfaj AA, Shi Y. 2024. Pelargonidin inhibits cell growth and promotes oxidative stress-mediated apoptosis in lung cancer A549 cells. Biotechnology and Applied Biochemistry 71, 1195–1203.

Zúñiga GE, Alberdi M, Fernández J, Móntiel P, Corcuera LJ. 1994. Lipid content in leaves of Deschampsia antarctica from the maritime Antarctic. Phytochemistry 37, 669–672.

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