Methane-Oxidizing Bacteria as Feed Replacement for Blue Mussel (Mytilus edulis) Larviculture

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Research Paper 01/12/2020
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Methane-Oxidizing Bacteria as Feed Replacement for Blue Mussel (Mytilus edulis) Larviculture

Charry Neleene Lagare Paracueles
Int. J. Biosci.17( 6), 266-275, December 2020.
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Bacterivory is common in bivalves. To test the effect of methane-oxidizing bacteria (MOB) on the growth and survival of blue mussel (Mytilus edulis) larvae, MOB was used as a replacement to microalgae. Enriched and sub-cultured MOB is from the marine sediment sample from the Northsea coast of Yeserke, The Netherland. The feeding ration of MOB was 75% 50% and 25% combined with 25%, 50% and 75% microalgae, respectively, based on dry weight; and control treatments are 50% and 100% microalgae only. The microalgal diet used consisted of a combination of Isosychris galbana. Chaetoceros muelleri and Tetraselmis suecica. Growth and survival of mussel larvae fed with MOB showed no significant difference compared with the 100% microalgae diets on day 6. Average shell height among all treatments was 80.7 ± 1.99 µm and an average survival rate of 11.12 ± 2.61%. Nevertheless, due to massive mortality due to Vibrio sp. attack in the laboratory. The experiment was terminated on day 8. This study, however, even at a short time still displayed that the use of MOB offers a promising result as a replacement of live algae for mussel larvae. The data of this study provide good insights regarding MOB as a possible bacterial meal for mussel larviculture. More future research is needed on the application of the MOB as feed for mussels since this is the first time that the MOB is applied as food for blue mussel larvae.


Aji LP. 2011. The Use of Algae Concentrate, dried algae and algal substitutes to feed bivales. Makara Journal of Science 13, 1-8.

Arapov J, Balic-Exgeta D, Glandan ZN. 2010. Bivalve Feeding: How and what they eat? Ribartsvo, 68, 105-116. CROSBI ID: 487017.

Beiras R, His E. 1994. Effects of dissolved mercury on embryogenesis, survival, growth and metamorphosis of Grassostrea gigas oyster larvae. Marine Ecology Progress Series 113, 95-103.

Bussman I, Pester M, Brune A, Schink B. 2004. Preferential cultivation of type II methanotrophic bacteria form littoral sediments (Lake Constance). Federation of Eropean Microbiological Societies, 1SX Microbiology Ecology 47, 179-189.

Cassandra AB, Lizarraga-Partida ML, Searcy-Bernal R. 2004. Effect of Vibrio alginolyticus on larval survival of the blue mussel Mytilus galloprovincialis. Diseases of Aquatic Organisms, 59(2), 110-23.

Coutteau P, Sorgeloos P. 1992. The use of algal substitutes and the requirement for live algae in the hatchery and nursery rearing of bivalve mullusc: an international survey. Journal of Shellfish Research 11(2), 467-476.

Dame R. 2012. Ecology of Marine Bivalves: An Ecosystem Approach. CRC Press.

Da Muller HN. 2006, April 5. Patent No. EP1641475 A1. USA.

Douillet P, Langdon C. 1993. Effects of marine bacteria on the culture of axenic oyster Crassostrea gigas (Thunberg) larvae. Biology Bulletin 184, 36-51.

Doroudi MS, Southgate PC, Mayer RJ. 1999. Growth and survival of blacklip pearl oyster larvae fed different densities of microalgae. Aquaculture International 7, 197-187.

Dubert J, Barja JL, Romalde JL. 2017. New Insights into Pathogenic Vibrios Affecting Bivalves in Hatcheries: Present and Future Prospects. Frontiers in Microbiology 8, 762.

Eggermont M, Tamanji A, Nevejan N, Bossier P, Sorgeloos P, Defoirdt T. 2014. Stimulation of heterotrophic bacteria associated with wild-caught blue mussel (Metylus edulis) adult’s results in mass mortality. Aquaculture 431, 136-138.

Eggermont M, Bossier P, Julyantoro-Pande G S, Delahaut V, Rayhan AM, Gupta N, Islam SS, Yumo E, Nevejan N, Sorgeloos P, Gomez-Gil B, Defoirdt T. 2017. Isolation of Vibrionaceae from wild blue mussel (Mytilus edulis) adults and their impact on blue mussel larviculture, Federation of European Microbiological Societies, Microbiology Ecology 93(4), April 2017, fix039,

Fitzpatrick J. 2015. How can I determine the bacterial biomass of my culture? [Msg 2]. Message posted at

Gatenby CM, Parker BC, Neves RJ. 1997. Growth and survival of juvenile rainbow mussels, Villosa iris (Lea 1829) (Bivalvia: Unionidae). Reared on algal diets and sediments. American Malacological Bulletin 4(1), 57-66.

Green P. 1992. Taxonomy of Methylotrophic Bacteria. In J. D. Murrell, Biotechnology Handbook 5: Methane and Methane Utilizers (p 23-77). New York: Plenum Press.

Ha D, Nachtergaele L, Kerckhof F, Ramelyanti D, Bossier P, Verstrate W, Boon N. 2012. Conversion of BIogas to bioproducts by algae and methane oxidizing bacteria. Environmental Science Technology 46, 13425-13432.

Heyer J, Galchenko VF, Dunfield. 2002. Molecular phylogeny of type II methane oxidizing bacteria isolated from various environments. Microbiology 2831-2846.

His E, Seaman MNL, Beiras R. 1997. A simplification the bivalve embryogenesis and larval development bioassay method for water quality assessment. Water Reservation 31, 351-355.

Ivanova EG, Fedorov DN, Doronina NV, Trotsenko Y. 2006. Production of Vitamin B12 in Aerobic methylotrophic bacteria. Microbiology 75, 494-496.

Ku´zniar A, Furtak K, Wlodarczyk, Stepniwska Z, Wolinksa A. 2019. Methanotrophic Bacterial Biomass as Potential Mineral Feed Ingredients for Animals. International Journal of Environmental Research and Public Health.

Leak DJ. 1992. Biotechnological and Applied Aspects of Methane and Methanol Utilizers. In: Murrell J.C., Dalton H. (eds) Methane and Methanol Utilizers. Biotechnology Handbooks 5, Springer, Boston, MA.

Leak DJ, Dalton H. 1986. Growth Yields of Methanotrophs. Applied Microbial Biotechnology, 23, 470–476.

Ruger M, Ackermann M, Reichl U. 2014. Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry. BMC Microbiology 14, 56.

 Rico-Villa B, Pouvreau S, Robert R. 2009. Influence of food density and temperature on ingestion, growth and settlement of Pacific oyster larvae, Crassostrea gigas. Aquaculture 287, 395-401.

SEAFDEC. 2014. SEAFDEC/AQD. Retrieved July 19, 2014, from

Simonsson D. 2013. Micro contamination routes and feed quality in hatchery culture Ostrea edulis larvae. Unpublished.

Toi HT, Boeckx P, Sorgeloos P, Bossier P, Van Stappen G. 2013. Co-feeding of microalgae and bacteria may result in increased N assimilation in Artemia as compared to mono-diets, as demonstrated by a 15N isotope uptake laboratory study. Aquaculture, 422-423.

Tomaru Y, Ichiro Z, Nakano S. 2000. Consumption of picoplankton by the bivalve larvae of Japanese pearl oyster Pinctada fucata martensii. Marine Ecology Progress Ecology Series 192, 195-202.

Wacker A, Von Elert E. 2004. Food quality controls quality of the zebra mussel Dreissena polymorpha: The role of fatty acids. Limnology Oceanography 49(5).

Whittenbury R, Phillipis KC. Wilkinson JF. 1970. Enrichment, Isolation and Some Properties of Methane-Utilizing Bacteria. Journal of General Microbiology 61(2), 203-128.

Елена Сергеевна БабусенкоВалерий, Алексеевич БыковНаталья Леонидовна Куликова, Маргарита Витальевна Лалова, Леонид Евгньевич Левитин, Александр Иванович Сафонов. 2020. Protein Feed Supplement for Farm Animals and Fish. Russia. March 27, 2020.