Microbiological risk characterization of select complementary alternative therapies for dengue fever in the Philippines

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Research Paper 01/02/2018
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Microbiological risk characterization of select complementary alternative therapies for dengue fever in the Philippines

Lucilyn Maratas, Dana Joanne Von Trono
J. Biodiv. & Environ. Sci. 12(2), 32-39, February 2018.
Copyright Statement: Copyright 2018; The Author(s).
License: CC BY-NC 4.0

Abstract

Dengue fever causes mortality and morbidity across the world and in the Philippines, there is widespread use of herbal-based medicines and medicinal plants to treat this infection. The research study was primarily undertaken to conduct a general microbiological evaluation of the local complementary alternative therapies (CAT) for dengue fever,  in order to determine its safety for human use and consumption. A preliminary question-based survey was conducted to assess the awareness and the commonly practiced CAT of the study population. 63% of the population reported to be aware of the potential of herbal plants for supplementary therapy particularly against dengue-induced thrombocytopenia. The general safety of three most commonly used herbal plants, Euphorbia hirta, Carica papaya and Ipomea batatas, (in forms of decoction and infusion) were assessed through employing heterotrophic plate counts (HPC) and antimicrobial screening. HPC evaluation showed varied levels of contamination ranging from >1000 to 3.0 x 102 CFU/ml, which could be attributed to unstandardized preparation procedures. All decoctions and infusions exhibited negative antimicrobial properties against Salmonella typhi, Escherichia coli and Staphylococcus aureus , hence, is advantageous as orally administered therapies must contain diminutive amount of antibacterial activity property in the intention that it will not invade other normal human cells except for transient and pathogenic bacterial cells.

Abd Kadir SL, Yaakob H, Mohamed Zulkifli R. 2013. Potential anti-dengue medicinal plants: a review. Journal of Natural Medicines 67, 677.  http://dx.doi.org/10.1007/s11418-013-076y

Acevedo S. 2010. Antimicrobial Properties of Various Green Tea Products with and without the Addition of Sugar, (Unpublished).

Aragones S. 2010. Can ‘tawa-tawa” cure dengue? Available at www.abscbnnews.com/lifestyle/09/09/10/can-tawa-tawa-cure-dengue.

Cavalieri SJ, Harbeck RJ, McCarter YS, Ortex JH, Rankin ID, Sautter RL, Sharp SE, Spiegel SA. 2005. Manual of antimicrobial susceptibility testing. American Society for Microbiology. American Society for Microbiology. ISBN 1-55581-349-6.

Elena F, Nessa Z, Ismail N. 2003. Antioxidant activity of extracts from the leaves of Azadarichta indica (neem) seeds extracts against bacterial pathogens associated with eye and ear infections. Journal of Medicinal Plants Research 4(14), 1414-1421. https://doi.10.5897/JMPR09.169.ISSN1996-0875

Guzman MG, Halstead SB, Artsob H, Buchy P, Farrar J, Gubler DJ, Kroeger A, Margolis HS, Martinez E, Nathan MB, Pelegrino JL, Simmons C, Yoksan S, Peeling RW. 2010. Dengue: A continuing global threat. Nature Reviews Microbiology 8:S7-S16.

Huang N, Hung W, Tsai W, Lai F, Lin Y, Huang M, Chen J, Lin W, Weng J, Chang T. 2017. Ficus septica plant extracts for treating Dengue virus in vitro. Peer Journal 5:e3448.  http://dx.doi.org/10.7717/peerj.34.48

Hussain SM, Ananthan G. 2012. Antimicrobial Activity of the Crude Extracts of Compound Ascidians, Didemnum candidu and Didemnum psammathodes (Tunicata: Didemnidae) from Mandapam (Southeast Coast of India). Current Research Journal of Biological Sciences.

International Centre for Science and High Technology-United Nations Industrial Development Organization (ICS-UNIDO). 2008. An Overview of Extraction Techniques for Medicinal and Aromatic Plants. Trieste, Italy: ICS-UNIDO.

Karishna KL, Paridhavi M, Pate JA. 2008. Review on nutritional, medicinal and pharmacological properties of papaya Carica papaya Linn. Natural Products Radiance 7, 364-373.

Krishna KL, Paridhavi M, Patel JA. 2008. Review on the nutritional, medicinal and pharmacological properties of papaya (Carica papaya Linn). Natural Products Radiance 7(4), 364-373.

Kunle OF, Egharevba HO, Ahmadu PO. 2012. Standardization of herbal medicines- A Review. International Journal of Biodiversity and Conservation 4(3), 101-112.

Leite AA, Nardi RM, Chartone- Souza E, Nascimenta AM. 2005. Carica papaya seed macerate as inhibitor of conjugative R plasmid transfer from Salmonella typhi to Escherichia coli, in vitro and in the digestive tract of genobiotic mice. The Journal of General and Applied Microbiology 51(1), 16-21.

Miano RS, Picardal JP, Alonso CAG, Reuyan D. 2011. Ethnobotanical Inventory and Assessment of Medically Important Plant Roots in Cebu Island, Philippines. Asian Journal of Biodiversity 2, 81-101.

Patil SB, Magdum CS, Naikwande NS. 2009. Review of Phytochemistry and Pharmacological aspects of Euphorbia hirta Linn. Journal of Pharmaceutical Research and Health Care 1, 113-133.

Philippine Council for Health Research and Development. 2012. R & D Thrusts on Dengue. Annual Report. Philippines: Department of Science and Technology. Retrieved from www.worldngayon.com/wpcontent/uploads/2012/05/JCMONTOYA_DOST-PCHRD-RD-on-Dengue_April12_Revised.pdf

Raintree Nutrition.  2012. Carica papaya. Tropical Plant Database. Available at www.rain-tree.com/papaya.htm

Rajeh MAB, Kwan YP, Zakaria Z, Latha LY, Jothy SL, Sasidharan S. 2012. Toxicity Impacts of Euphorbia hirta L. extract of Behavior, Organs Body Weight Index and Histopathology of Organs of the Mice and Artemiasalina. Pharmacognosy Research 4(3), 170-177. http://dx.doi.org/10.4103/0974-8490.99085.

Sathasivam K, Ramanathan S, Mansor SM, Rosemal M, Wernsdofer WH. 2004. Thrombocyte counts in mice after the administration of papaya leaf suspension. Wiener Klinische Wochenschrift 121(3), 19-22.

United States Pharmacopia.  2009. Chapter 61: Microbiological Examination of Non Sterile Products: Microbial Enumeration Test. https://hmc.usp.org/sites/default/files/documents/HMC/GCs-Pdfs/c61.pdf

Vieira RHS, Rodrigues D. Goncalves FA, Menezes FGR, Aragao JS, Sousa OV. 2001. Microbicidal effect of medicinal plant extracts (Psidium guajava Linn and Carica papaya Linn) upon bacteria isolated form fish muscle and known to induce diarrhoea in children 43, 145-148.

World Health Organization. 2003. Guidelines on Good Agricultural and Collection Practices for Medicinal Plants. Geneva, World Health Organization. www.whqlibdoc.who.int/publications/2003/9241546271.pdf

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