Micropropagation of Prosopis cineraria (L.) Druce, An endangered tree of Pakistan

Paper Details

Research Paper 01/06/2018
Views (663)
current_issue_feature_image
publication_file

Micropropagation of Prosopis cineraria (L.) Druce, An endangered tree of Pakistan

Adeela Haroon, Naila Manzoor, Muzafar Iqbal
Int. J. Biosci. 12(6), 443-449, June 2018.
Copyright Statement: Copyright 2018; The Author(s).
License: CC BY-NC 4.0

Abstract

The present work was carried out to develop some newer techniques for the propagation of the endangered tree, Prosopis cineraria. For this reason, forcing of softwood shoots from large stem segments of P. cineraria was done. During dormant season, shoot were forced from large stem segments of jand. The large stem segments were placed in different growth media (Sand, peat moss and sawdust) under different environmental conditions (Dark and Natural). Results showed that peat moss logs produced the maximum number of shoots as compared to other tested media, sand and sawdust under both environmental conditions. Environmental conditions also had a significant effect as the dark condition gave promising results. Moreover, under natural condition, a fair number of shoots were produced in all media. But the sprouted logs soon turned dried due to harsh environmental conditions. While, the sprouted logs under dark conditions, on transferring into natural environment endured the harsh environment and established well. Therefore, the results suggests that this simple micropropagation technique developed for P. cineraria from stem cuttings can be exercised for large-scale rapid increase of this endangered tree.

Aftab F, Mansouri K, Preece JE. 2005. The influence of environment, media and Zertol on forcing and in vitro establishment of softwood shoots from large segments of Acer saccharinum L. and Fraxinus pennsylvanica Marsh. Propagation of Ornamental Plants 5(3), 111-116.

Akram M, Aftab F. 2009. An efficient method for clonal propagation and in vitro establishment of softwood shoots from epicromic buds of teak (Tectona grandis L.). Forestery Studies in China. 11(2), 105-110.

Ali SI. 2015. Flora of Pakistan. Department of Botany, University of Karachi  p36.

Arora K, Sharma M, Srivastava J, Ranade SA, Sharma AK. 2010. Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments. Agroforestry Systems 78(1), 53-63.

Arya ID, Nautiyal S, Arya S. 2013. Tissue culture studies on clonal variations in micropropagation of Dalbergia sissoo. International Journal of Biotechnology Research 1(4), 58-70.

Benson EE. 2000. Special symposium: In vitro plant recalcitrance. An introduction. In vitro Cellular and Development Biology-Plant 36(3), 141-148.

Bovo Oa, Castillo G, Zibetti G, Muzzio P. 1991. Cultivo in vitro de tejidosdelgéneroProsopis (Leguminosae). ActasJornadas Taller Com. Nac.deProsopis. Sgo.del Estero, pág. 28.

Gehlot A, Arya ID, Kataria V, Gupta RK, Arya S. 2014. Clonal multiplication of multipurpose desert tree Azadirachta indica – Neem. Journal of Arid Land Studies 24(1), 37-40.

Henry PH, Preece JE. 1997. Production of shoots from dormant species of Acer as influenced by length and caliper of stem sections. Journal of Environmental Horticulture 15, 153-156.

Janbaz K H, Haider S, Imran I, Haq MZU, Martino LD, Feo VD. 2012. Pharmacological evaluation of Prosopis cineraria (L.) Druce in gastrointestinal, respiratory and vascular disorders. Evidence-based Complementary and Alternative Medicine 7.

Jordan M, O Balbao. 1985. In vitro Regeneration of Prosopistamarugo Phil. And Prosopischilensis (Mol.) Stuntz from Nodal Sections/In vitro-Regenerierung von Prosopistamarugo und ProsopischilensisausNodien-Segmenten. Gartenbauwissenschaft 138-141.

Kiritkar KR, Basu BD. 1984. Indian medicinal plants. International Book Distributors, Dehradun   2, 910-911.

Kumar S , Singh N. 2009. Micropropagation of Prosopis cineraria (L.) Druce- A multipurpose desert tree. Reasercher 1(3), 28-32.

Mahoney D. 1990. Trees of Somalia – A field guide for development workers. Oxfam/HDRA, Oxford. p. 133-136.

Malik A, Kalidhar S. 2007. Phytochemical examination of Prosopis cineraria L. (Druce) leaves. Indian Journal of Pharmaceutical Sciences 69, 619-622.

Misra AN, Misra M. 2012. Sterilization Techniques in Plant Tissue Culture. In book: Plant Tissue culture: Totipotency to Transgenic. Publisher: Agrobios (India). Chapter 3, pp. 31-42.

Muturi GM, Poorter L, Mohren GMJ, Kigomo BN. 2013. Ecological impact of Prosopis cineraria species invasion in Turkwel riverine forest, Kenya. Journal of Arid environments 92, 89-97.

Orwa C, Mutua A, Kindt R, Jamnadass R, Anthony S. 2009. Agroforestry database: A tree reference and selection guide version 4.0.

Pareek AK, Garg DS, Kumar M, Yadav SM. 2015. Prosopis cineraria: A gift of nature for pharmacy. International Journal of Pharma Science and Research 6, 958-964.

Pasiecznik NM, Harris PJC, Smith SJ. 2004. Identifying tropical Prosopis species A Field Guide. Coventry, England: HDRA Publishing.

Pathak D, Agnihotri R K, Sharma R. 2016. Callus regeneration of shami (Prosopis cineraria) an endangered plant of Braj region of Uttar Perdesh. The Journal of Plant Science Research. 32(1), 95.

Phulwaria M, Rai MK, Harish, Gupta AK, Ram K, Shekhawat Ns. 2012. An improved micropropagation of Terminalia bellirica from nodal explants of mature tree. Acta Physiologiae Plantarum, 34(1), 299-305.

Phulwaria M, Ram K, Gahlot P, Shekhawat NS. 2011. Micropropagation of Salvadora persica – a tree of arid horticulture and forestry. New Forests 42(3), 317-327.

Preece JE, Read PE. 2003. Novel methods in micropropagation. Proceedings of the International Symposium on acclimatization and establishment of micropropagated plants. Acta Horticulture. 616, 71-76.

Rani B, Singh U, Sharma R, Gupta A, Dhawan NG, Sharma AK, Sharma S, Maheshwari RK. 2013. Prosopis cineraria (L) Druce: A desert to brace livelihood in Rajasthan. Asian Journal of Pharmaceutical Research and Health Care 5, 58-64.

Rathore TS, Deora NS, Shekhawat NS. 1992. Cloning of Maytenus emarginata (Willd.) Ding Hou – a tree of the Indian desert, through tissue culture. Plant Cell Reports 11(9), 449-451.

Shekhawat Ns, Rathore Ts, Singh Rp, Deora Ns, Rao SR. 1993. Factors affecting in vitro clonal propagation of Prosopis cineraria. Plant Growth Regulators 12, 273-280.

Related Articles

Rice productivity and soil hydrodynamic properties under lowland Elaeis guineensis and Borassus aethiopum: An asset rice agroforestry system

Sissou Zakari, Pierre Tovihoudji, Janvier Egah, Sékaro Amamath Boukari, Raymon Bio Gonga, Mouiz W. I. A. Yessoufou, Imorou F. Ouorou Barrè, Int. J. Biosci. 27(4), 177-190, October 2025.

Population dynamic parameters for Pseudupeneus prayensis (Mullidae) (Cuvier, 1829) in Ivorian continental shelf

Christian Bernard Tia, Abdoulaye Kone, Diomande Loua, Soumaïla Sylla, Essetchi Paul Kouamelan, Int. J. Biosci. 27(4), 169-176, October 2025.

Evaluation of phytochemicals and in vitro biological activities of Semecarpus kurzii leaf extract

Deepika, V. Ambikapathy, S. Babu, A. Panneerselvam, Int. J. Biosci. 27(4), 159-168, October 2025.

Comparative analysis of the influence of temporal, seasonal and behavioral factors using XGBoost for predicting traveled distances

Y. F. Lankoande, A. K. Gandema, S. Ouedraogo-Kone, A. Kone, Y. Sawadogo, J. Moses, Int. J. Biosci. 27(4), 150-158, October 2025.

The protective effect of black pepper (Piper nigrum) on liver enzymes in streptozotocin-induced diabetic rats

Amani A. R. Filimban, Khulud A. Wathi, Int. J. Biosci. 27(4), 140-149, October 2025.

Production of bioplastics (PHB) using waste paper as feed stock by Cupriavidus taiwanensis

Ajeena Davis, Jini Joseph, Int. J. Biosci. 27(4), 130-139, October 2025.

Gill ectoparasites of the mugilidae from the Ebrié lagoon, Abidjan (Côte d’Ivoire)

Eby Yoboué Gnamma Honorine Alla, Carel Wilfried Bermian Dibi-Ahui, Fidèle Kouassi Kouakou, Abouo Béatrice Adepo-Gourene, Int. J. Biosci. 27(4), 123-129, October 2025.

Impacts of diverse water management systems on growth and yield of two prominent boro rice cultivars in Bangladesh

Zahidul Islam, Md Ekhlasur Rahman, Md Khayrul Islam Bashar, Sharmin Sultana, Md Taharat Al Tauhid, Md Rabiul Islam, Md Shahed Hossain, Md Musa Mondal, Pradip Kumar Biswas, Int. J. Biosci. 27(4), 110-122, October 2025.