Molecular and physiological responses of Pisum sativum and Vicia faba to sodium azide

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Research Paper 01/06/2014
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Molecular and physiological responses of Pisum sativum and Vicia faba to sodium azide

M. Hamouda, K.M. Saad-Allah, W.A. Kasim
Int. J. Agron. Agri. Res.4( 6), 46-61, June 2014.
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Abstract

Seeds of Pisum sativum and Vicia faba were pre-soaked in either 1.0, 2.0 or 4.0 mM of sodium azide (NaN3) for 30 min, 1 h or 2 hs then, sown in clay soil in plastic pots under conditions of 25/18°C day/night and natural light (16/8 h day/night) and the seedlings were harvested after 21 days. The results revealed that total phenols, H2O2, malondialdehyde and the activities of catalase, peroxidase and DPPH scavenging activity in both plant were gradually increased with the increase in NaN3 concentration and soaking time. The isozymes of α- and β-esterases and α-amylase had number of loci ranged from two (α-amylase) to four (α-esterase) in pea, while in bean, it was three for the three investigated enzymes. The ISSR analysis revealed that both plants showed highly significant increase in the appearance of bands by the highest treatment of NaN3, especially with the longest duration. Pea produced 14 polymorphic, 9 monomorphic and 4 unique bands, while bean produced 14 polymorphic, 8 monomorphic and 3 unique bands.

VIEWS 5

Allendorf FW, Luikart G. 2007. Conservation and the Genetics of Populations. Blackwell Publishing, Malden, Massachusetts, USA. p. 642.

Al-Qurainy F, Khan S. 2009. Mutagenic effects of sodium azide and its application in crop improvement. World Applied Science Journal 6, 1589–1601.

Aly A, Elsayed HE. 2006. Molecular analysis of two bananas genotypes induced in vitro under gamma rays stress. Egyptian Society of Nuclear Science and Application 39, 188-194.

Ashwani P, Single SL, Grover A. 1998. Proteins alterations associated with salinity, desiccation, high and low temperature stresses and abscisic acid application in seedlings of pusa 169, a high yielding rice (Oryza sative L.) cultivars. Current Sciences 75 (34), 1023-1035.

Azevedo Neto AD, Prisco JT, Eneas-Filho J, Medeiros JR, Gomes-Filho E. 2005. Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants. Journal of Plant Physiology 162, 1114-1122.

Bailly C, Benamar A, Corbineau F. 1996. Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiologia Plantarum 97, 104-110.

Bartosova Z, Obert B, Takac T, Kormutak A, Pretova A. 2005. Using enzyme polymorphism to identify the gametic origin of flax regenerants. Acta Biologica Cracoviensia series Botanica 47(1), 173-178.

Beauchamp CO, Fridovich I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44, 276-287.

Berenschot AS, Zucchi MI, Tulmann-Neto A, Quecini V. 2008. Mutagenesis in Petunia x hybrida Vilm. and isolation of a novel morphological mutant. Brazelian Journal of Plant Physiology 20 (2), 16–27.

Birosov L, Mikulasov M, Vaverkov S. 2005. Antimutagenic effect of phenolic acids. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 149 (2), 489–491.

Bondet V, Brand-Williams W, Berset C. 1997. Kinetics and mechanisms of antioxidant activity using the DPPH* free radical method. Lebensmittel-Wissenschaft and Technologie 30 (6), 609-615.

Bradley DE, Min DB. 1992. Singlet oxygen oxidation of foods. Critical Reviews in Food Science and Nutrition 31, 211-236.

Brady L, Brzozowski AM, Derewenda ZS, Dodson E, Dodson G. 1990. A serine protease triad forms the catalytic centre of a triacylglycerol lipase. Nature 343, 767–770.

Brand-Williams  W,  Cuvelier  ME,  Berset  C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft and Technologie 28, 25-30.

Brune M, Rossander L, Hallberg L. 1989. Iron absorption and phenolic compounds: importance of different phenolic structures. European Journal of Clinical Nutrition 43, 547–557.

Caturla N, Vera-Samper E, Villalain J, Reyes Mateo C, Micol V. 2003. The relationship between the antioxidant and antibacterial properties of galloylated catechins and the structure of phospholipid model membranes. Free Radical Biology and Medicine 34, 648-662.

Chang C, Yang M, Wen H, Chern J. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis 10, 178-182.

Chaurasia PK, Bharati SL, Singh SK, Yadav RS. 2013. A Potential Peroxidase Obtained from the Juice of Beta vulgaris (Beet). American Journal of Food Science and Technology 1 (3), 30-35.

Crepona K, Marget P, Peyronnet C, Carroueea B, Arese P, Duc G. 2010. Nutritional value of faba bean (Vicia faba L.) seeds for feed and food. Field Crop Research 115, 329-339.

de Mejia EG, Castano-Tostado E, Loarca-Pina G. 1999. Antimutagenic effects of natural phenolic compounds in beans. Mutation Research 441, 1–9.

Deka R, Guangyun S, Smelser D, Zhong Y, Kimmel M, Chakraborty R. 1999. Rate and Directionality of Mutations and Effects of Allele Size Constraints at Anonymous, Gene-Associated, and Disease-Causing Trinucleotide Loci. Molecular Biology and Evolution 16 (9), 1166–1177.

Del Pozo-Insfran D, Brenes CH, Talcott ST. 2004. Phytochemical composition and pigment stability of acuai (Euterpe oleracea Mart.). Journal of Agricultural and Food Chemistry 52, 1539-1545.

Dixon RA, Paiva NL. 1995. Stress-induced phenylpropanoid metabolism. Plant Cell 7, 1085-1097.

Dogan B, Duran A, Hakki EE. 2007. Phylogenetic analysis of Jurinea (Asteraceae) species from Turkey based on ISSR amplification. Annales Botanici Fennici 44, 353-358.

Duhan JS, Bhardwaj M, Surekha S. 2011. Free radical-scavenging and antimutagenic potential of acetone, chloroform and methanol extracts of fruits of Argemone mexicana. African Journal of Biotechnology 10 (43), 8654-8661.

Edenharder R, van Petersdorf I, Rauscher V. 1993. Antimutagenic effects of flavonoids, chalcones and structurally related compounds on the activity of 2-amino-3-methylimidazol (4,5-f) quinoline (IQ) and other heterocyclic amine mutagens from cooked food. Mutation Research 287, 261–274.

EL-Fadly G, Sidaros S, Deif AA. 1990. Effect of boistin on gene expression and yield components of faba bean (Vicia faba) infected with broad bean strain virus. Proc. 3th Conf. Agric. Dev. Res. Faculty of Agriculture, Ain shams University, Cairo, Egypt.

El-Mokadem H, Mostafa, G. 2014. Induction of mutations in Browallia speciosa using sodium azide and identification of the genetic variation by peroxidase isozyme. African Journal of Biotechnology 3(1), 106-111.

Elzebroek T, Wind K. 2008. Guide to cultivated plants. CAB International, Oxfordshire, UK.

Foyer CH, Noctor G. 2005. Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environment 28, 1056–1071.

Francisco J, Perez RV, Sebastian R. 2008. H2O2 is involved in the dormancy-breaking effect of hydrogen cyanamide in grapevine buds. Plant Growth Regulation 55, 149-155.

Friedman MN. 1997. Chemistry, biochemistry and dietary role of potato polyphenols – a review. Journal of Agricultural and Food Chemistry 45, 1523–1540.

Frigo MJ, Mangolin CA, Oliveira RS, Machado MF. 2009. Esterase polymorphism for analysis of genetic diversity and structure of wild poinsettia (Euphorbia heterophylla) populations. Weed Science 57, 54-60.

Gao Y, Guo Y, Lin S, Fang Y, Bai J. 2010. Hydrogen peroxide pretreatment alters the activity of antioxidant enzymes and protects chloroplast ultrastructure in heat-stressed cucumber leaves. Scientia Horticulturae 126, 20-26.

Gechev TS, Hille J. 2005. Hydrogen peroxide as a signal controlling plant programmed cell death. Journal of Cell Biology 168, 17-20.

Gowri S, Chinnaswamy P. 2011. Evaluation of in vitro antimutagenic activity of Caralluma adscendens Roxb. in bacterial reverse mutation assay. Journal of Natural Products and Plant Resources 1 (4), 27-34.

Grace SC, Logan BA. 2000. Energy dissipation and radical scavenging by the plant phenylpropanoid pathway. Philosophical Transactions of the Royal Society B 355, 1499-1510.

Gupta MY, Chyi SJ, Romero-Severson J. 1994. Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple sequence repeat. Theoretical and Applied Genetics 89, 998-1006.

Ho C, Lee CY, Huang M. 1992. Phenolic Compounds in Food and their Effects on Health I. Analysis, Occurrence and Chemistry. Washington, DC: American Chemical Society. pp 1-7.

Horn1 RC, Vargas VMF. 2003. Antimutagenic activity of extracts of natural substances in the Salmonella/microsome assay. Mutagenesis 18 (2), 113–118.

Hour T, Liang Y, Chu I, Lin J. 1999. Inhibition of eleven mutagens by various tea extracts, (-) Epigallocatechin-3-gallate, Gallic Acid and Caffeine. Food and Chemical Toxicology 37, 569-579.

Ilbas AI, ErogluY, Eroglu HE. 2005. Effects of the application of different concentrations of NaN3 for different times on the morphological and cytogenetic characteristics of barley (Hordeum vulgare L.) seedlings. Journal of Integrative Plant Biology 47, 1101-1106.

Jain SC, Agrawal M. 1990. Effect of sodium azide on pharmaceutically active flavonoids in Trigonella species. Indian Journal of Pharmaceutical Sciences 52(1), 17-19.

Janero DR. 1990. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radical Biology and Medicine 9, 515-540.

Jeng TL, Shih YJ, Lai CC, Wu MT, Sung JM. 2010. Ant-oxidative characterization of NaN3 induced common bean mutants. Food Chemistry 119, 1006-1011.

Jindal KK, Singh RN. 1975. Phenolic content in male and female Carica papaya: A possible physiological marker for sex identification of vegetable seedlings. Physiologia Plantarum 33, 104-107.

Kagan VE, Tyurinov YY. 1998. Recycling and redox cycling of phenolic antioxidants. Annals of the New York Academy of Sciences, 854, 425–434.

Kato M, Shimizu S. 1987. Chlorophyll metabolism in higher plants. VII. Chlorophyll degradation in senescing tobacco leaves: phenolic-dependent peroxidative degradation. Canadian Journal of Botany 65, 729-735.

Kleinhofs A, Warner RL, Muehlbauer FJ, Nilan RA. 1978. Induction and selection of specific gene mutations in Hordeum and Pisum. Mutation Research 51, 29–35.

Kleinhofs W, Sander C. 1975. Azide mutagenesis in barley. Third Barley Genetics Symp. Garching. Proceedings of Symp. pp 113-122.

Kumar CN, Knowles RN. 1993. Changes in lipid peroxidation and lipolytic and free radical scavenging enzyme activities during ageing and sprouting of potato (Solanum tuberosum) seed tubers. Plant Physiology 102, 115-124.

Malaviya DR, Roy AK, Tiwari A, Kaushal P, KumarB. 2006. In  vitro  callusing  and regeneration  in Trifolium  resupinatum  -a  fodder legume. Cytologia 71, 229-235.

Maxim E, Oprica L, Caprarum G, Truta E, Artenie V. 2009. Action of caffeine and sodium azide on activity of some antioxidative enzymes in Carum carvi L. seedlings. Analele Ştiinţifice ale Universităţii, Alexandru Ioan Cuza, Secţiunea Genetică şi Biologie Moleculară, TOMX 63-86.

Micales JA, Bonde MR, Peterson GL. 1992. Isozyme analysis in fungal taxonomy and molecular genetics. In: Arora DK, Elander R P, Mukerja KG (eds.). Handbook of applied mycology. Fungal biotechnology. Marcel Dekker Inc., New York. 4, 57-79.

Mierlici1 ID, Ciornea E, Ghiorghi G. 2011. The activity of some oxidoreductases in Hordeum vulgare L. plants treated with ethyl–methane-sulfonate and rosmarinus officinalis l. hydro-alcoholic extracts. Analele Stiintţifice ale Universitaţii, Alexandru Ioan Cuza”, Secţiunea Genetica si Biologie Moleculara 12(1), 67-72.

Mihara  M, Uchiyama M. 1978. Determination of malondialdehyde precursor in tissues by thiobarbituric acid test. Analytical Biochemistry 86, 271–278.

Murthy UMN, Kumar PP, Sun WQ. 2003. Mechanisms of seed ageing under different storage conditions for Vigna radiate (L.) Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition. Journal of Experimental Botany 54, 1057–1067.

Mydlarz LD, Harvell CD. 2006. Peroxidase activity and inducibility in the sea fan coral exposed to a fungal pathogen, Comparative Biochemistry and Physiology, Part A 146, 54–62.

Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni PP, Biyani MK. 2003. Comparative antioxidant activity of individual herbal component used in Ayurvedic medicines. Phytochemistry 63, 97-104.

Olayinka AA, Anthony IO. 2010. Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary and Alternative Medicine 10, 21.

Owais WM, Kleinhofs A. 1988. Metabolic activation of the mutagen azide in biological systems. Mutation Research 197, 313–323.

Padmanaban V, Karthikeyan R, Karthikeyan T. 2013. Differential Expression and Genetic Diversity Analysis Using Alpha Esterase Isozyme Marker in Ocimum sanctum L. Academic Journal of Plant Sciences 6(1), 01-12.

Pandey A, Benjamin S, Soccol CR, Nigam P, Krieger N. 1999. The realm of microbial lipases in biotechnology. Biotechnology and Applied Biochemistry 29, 119–131.

Pasteur N, Pasteur G, Bonhomme F, Calalan J, Britton-Davidson J. 1988. Pratical isozyme genetics. The English Edition, Ellis Horwood Ltd., England.

Phapale R, MisraThakur S. 2010. Antioxidant activity and antimutagenic effect of phenolic compounds in Feronia limonia (L) swingle fruit. International Journal of Pharmacy and Pharmaceutical Sciences 2(4), 68-73.

Potapovich AI, Kostyuk VA. 2003. Comparative study of antioxidant properties and cytoprotective activity of flavonoids. Biochemistry 68, 514-519.

Price AH, Hendry GAF. 1991. Iron catalase oxygen radical formation and its possible contribution to drought damaged in nine native grasses three cereals. Plant Cell and Environment 14, 477-484.

Quan L, Zhang B, Shi W, Li H. 2008. Hydrogen peroxide in plants: a versatile molecule of the reactive oxygen species network. Journal of Integrative Plant Biology 50, 2-18.

Rekka E, Kourounakis PN. 1991. Effect of hydroxyethyl rutosides and related compounds on lipid peroxidation and free radical scavenging activity. Some structural aspects. Journal of Pharmacy and Pharmacology 43, 486-491.

Rice-Evans C, Miller N, Paganga G. 1997. Antioxidant properties of phenolic compounds. Trends in Plant Science 2, 152-159.

Saad-Allah KM, Hammouda M, Kasim WA. 2014. Effect of sodium azide on growth criteria, some metabolites, mitotic index and chromosomal abnormalities in Pisum sativum and Vicia faba. International Journal of Agronomy and Agricultural Research 4(4), 129-147.

Sander C, Nillan RA, Kleinhof SA, Vig BK. 1978. Mutagenic and chromosome-breaking effects of azide in barley and human leukocytes. Mutation Research 50, 67-76.

Santos RX, Melo SCO, Cascardo JCM, Brendel M, Pungartnik C. 2008. Carbon source-dependent variation of acquired mutagen resistance of Moniliophthora perniciosa: Similarities in natural and artificial systems. Fungal Genetics and Biology 45, 851–860.

Semagn K, Bjornstad A, Ndjiondjop MN. 2006. An Overview of Molecular Marker Methods for Plants. African Journal of Biotechnology 5, 2540-2568.

Sharma A, Namdeo A, Mahadik KR. 2008. Molecular Markers: New Prospects in Plant Genome Analysis. Pharmacognosy Reviews 2, 23-34.

Shehab AS, Tawab SAF, Morci MM. 2004. Stimulation of cell division and gene expression in Vicia faba L. using leaf powder of Azadirachte Indica. Egyptian Journal of Biotechnology 17, 499-514.

Singh R, Singh S, Kumar S, Arora S. 2007. Free radical scavenging activity of acetone extract/fraction of Acacia auriculiformis A. Cunn. Food Chemistry 103, 1403-1410.

Soliman MSA. 2003. Environmental impact of mobile communication tower on barley and broad bean plants. Bull. Fac. Sci. Assiut Univ., 32(2-D), 79-89.

Soltis DE, Haufler CH, Dartow DC, Gastony GJ. 1983. Starch gel electrophoresis of ferns: A compilation of grinding buffer. Gel and electrode, buffers and staining schedules. American Fern Journal 73, 9-27.

Szatrowski TP, Nathan CF. 1991. Production of Large Amounts of Hydrogen Peroxide by Human Tumor Cells. Cancer Research 51, 794-798.

Talukdar D. 2010. Allozyme variations in leaf esterase and root peroxidase isozymes and linkage with dwarfing genes in induced dwarf mutants of grass pea (Lathyrus sativus L.). International Journal of Genetics and Molecular Biology 2(6), 112-120.

Velikova V, Yordanov I, Edreva A. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Plant Science 151, 59-66.

Yadav RS, Yadav KS, Yadav HS. 2011. Luffa aegyptiaca (Gourd) Fruit Juice as a Source of Peroxidase. Enzyme Research 2011, 1-5.

Yamaguchi M, Kanemori T, Kanemaru M, Takai N, Mizuno Y, Yoshida H. 2004. Performance evaluation of salivary amylase activity monitor. Biosensors and Bioelectronics 20 (3), 491-97.

Yen GC, Chen HY. 1996. Relationship between antimutagenic activity and major components of various teas. Mutagenesis 11, 37–41.

Yerra R, Gupta M, Upal KM. 2005. Antitumor activity and in vivo antioxidant statut of Mucuna pruriens (Fabaceae) seeds against ehrlich ascites carcinoma in swiss albinos mice. Iranian Journal of Pharmacology and Therapeutics 4, 46-53.

Zaka R, Vandecasteele CM, Misset MT. 2002. Effects of low chronic doses of ionizing radiation on antioxidant enzymes and G6PDH activities in Stipa capillata (Poaceae). Journal of Experimental Botany 53, 1979–1987.

Zhang BH. 2000. Regulation of plant growth regulators on cotton somatic embryogenesis and plant regeneration. Biochemistry 39(6), 1567-1567.

Zhou YC, Zheng RL. 1991. Phenolic compounds and an analog as superoxide anion scavengers and antioxidants. Biochemical Pharmacology 42, 1177–1179.