Molecular characterization of a new allergen from Kochia scoparia pollen, Koc s 1
Paper Details
Molecular characterization of a new allergen from Kochia scoparia pollen, Koc s 1
Abstract
Kochia scoparia pollen is one of the primary sources of allergen that is distributed in tropical and subtropical areas of the world and has been identified as a potent allergen source, particularly in mid-summer. This study was designed to produce the allergenic Ole e 1-like protein from the pollen of K. scopariafor the evaluation of its IgE-binding capacity.Following subcloning of the coding sequence of K. scoparia Ole e 1-like protein into pTZ57R/T vector, the recombinant Ole e 1- like protein (rKoc s 1) was expressed in Escherichia coliusing pET-21b(+) vector. The immunoreactivity of rKoc s 1 was investigated bya specific-ELISA,immunoblotting and inhibition assays using32 sera collected from patients who were sensitised to theK. scopariapollen. Nucleotide sequencing showed an open reading frame of 507 base pairs encoding for 168 amino acid residues that pertained to the Ole e 1-like protein family. The immunoassays showed that 11 patients (34.37%) had considerable specific IgE levels to the rKoc s 1. The amino acid sequences of Koc s 1 showed high homology with selected allergenic members of the Ole e 1-like protein family.Koc s 1, the second allergen from the K. scopariapollen was produced and recognised as a member of the Ole e 1-like protein family. rKoc s 1 could be used as a tool for specific diagnosis and structural studies of allergy to K. scoparia.
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