Surveillance and detection of the occurrence of African swine fever in abattoirs in the different municipalities of the second district of Cagayan, Philippines

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Research Paper 12/03/2026
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Surveillance and detection of the occurrence of African swine fever in abattoirs in the different municipalities of the second district of Cagayan, Philippines

Maricel F. Campanano, Dennis M. Oyardo, Mary Ann M. Santos*
Int. J. Biosci. 28(3), 106-114, March 2026.
Copyright Statement: Copyright 2026; The Author(s).
License: CC BY-NC 4.0

Abstract

African swine fever (ASF) is a highly contagious hemorrhagic viral disease of domestic and wild pigs, which is responsible for serious economic and production losses. It is caused by a large DNA virus of the Asfarviridae family.  The study was conducted to determine positive   cases of African swine fever on pigs from different slaughterhouses in municipalities of second district in Cagayan. A total of 362 blood samples were collected in 6 municipal slaughterhouses. The samples were analyzed in the Regional Animal Disease Diagnostic Laboratory (RADDL). The viral DNA of ASF was extracted from the blood samples using the QIAamp Viral RNA Mini Kit (QIAGEN) and was identified through the RT-PCR (ASFV p72 gene-based real-time PCR assay). Two municipalities involve in the study resulted positive of African Swine Fever with a total of 60 out of 362 pigs. The municipality with the highest positive case and incidence rate is Sto. Nino with 41 out of 56 pigs are infected (incidence rate is 11.32%) followed by Piat with 19 pigs out of 96 pigs (incidence rate is 5.25%) and the rest no incidence of African Swine-Fever. The result obtained from the study indicates that out of 230 pooled samples (362 pigs), 35 (60 pigs) are showing 15.22% positivity rate and 16.57% incidence.

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