Molecular characterization of Escherichia coli resistance genes in chicken meat
Paper Details
Molecular characterization of Escherichia coli resistance genes in chicken meat
Abstract
Escherichia coli gene mutations (plasmids, integrons, and transposons) have instigated multidrug resistance (MDR) against various antimicrobials. This study detected antibiotic-resistance genes (genotypic and phenotypic) in E. coli and performed Whole-genome sequencing to discover MDR-associated E. coli genes in chicken meat. E. coli isolates were serologically identified, and their antimicrobial sensitivity was tested. E. coli presence was confirmed in 40% of the chicken samples. Cephalosporins, tetracyclines, and sulfonamides presented higher efficiency against E. coli. The serological investigation revealed the presence of STEC (O157:H7, 30%), ETEC (O142, 30%), EHEC (O26:H11, 10%), and EPEC (10%) in chicken meat samples. A symmetrical band represented the Subunit B of the Shiga-like toxin (SLT) gene whereas the Heat-labile toxin (LT) gene was found in plasmid and genomic DNA-detected strains. The results revealed the hazardous nature of STEC for chicken meat consumers. The study recommends improving the hygienic conditions during the chicken handling and processing steps, which will minimize antibiotic usage and resistance.
Abo-Amer AE, Shobrak AD, Altalhi MY. 2018. Isolation and antimicrobial resistance of Escherichia coli isolated from farm chickens in Taif, Saudi Arabia. J. Glob. Antimicrob. Resist.15, 65-68.
Adeyanju GT, Ishola AO. 2014. Salmonella and Escherichia coli contamination of poultry meat from a processing plant and retail markets in Ibadan, Oyo State, Nigeria. Springerplus 3, 139.
Ahmed AM, Shimamoto TM, Shimamoto AT. 2013. Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers. Int J Med Microbiol. 303, 475–83.
Ahmed AM, Shimamoto AS. 2015. Molecular analysis of multidrug resistance in Shiga toxin-producing Escherichia coli O157:H7 isolated from meat and dairy products. Int J Food Microbiol. 193, 68–73.
Capita RA, Alonso-Calleja MA, Prieto CM. 2007. Prevalence of Salmonella enterica serovars and genovars from chicken carcasses in slaughterhouses in Spain. J Appl Microbiol. 103, 1366–75.
Cunrath OA, Meinel PM, Maturana JA, Fanous JA, Buyck PM, Saint AM, Auguste BA, Helena SA, Smith JA, Körner CA, Dehio VA, Trebosc CA, Kemmer RN, Neher RA, Egli AA, Bumann CM. 2019. Quantitative contribution of efflux to multi-drug resistance of clinical Escherichia coli and Pseudomonas aeruginosa strains. BioMedicine. 41, 479-487.
Gao JA, Hu LM, Zhang XA. 2015. Application of swine manure on agricultural fields contributes to extended- spectrum ßlactamase producing Escherichia coli spread in Tai’an, China. Frontiers in Microbiology 6, 313-318.
Habib AA, Afroz MA, Rahman DA, Begum MN, Kamruzzman AA, Haque FA. 2021. Molecular characterization and antimicrobial resistance of Escherichia coli isolated from retail chicken meat. South Asian J. Bio. Res. 4 (2), 38-47.
Hansen KH, Bortolaia CA, Nielsen JB, Nielsen KA, Schonning YA, Agerso VA. 2016. Host-specifc patterns of genetic diversity among IncI1-Igamma and IncK plasmids encoding CMY-2 B-lactamase in Escherichia coli isolates from humans, poultry meat, poultry, and dogs in Denmark. Appl. Environ. Microbiol. 82, 4705–14.
Hernandez TA, Sierra CA, Rodriguez-Alvarez AM, Torres MP, Arevalo MA, Calvo AM. 2005. Salmonella enterica serotypes isolated from imported frozen chicken meat in the Canary Islands. J. Food Prot. 68, 2702–6.
Johnson TJ, Wannemuehler CA, Doetkott SJ, Johnson SC, Rosenberger LK, Nolan YA. 2008. Identification of Minimal Predictors of Avian Pathogenic Escherichia coli Virulence for Use as a Rapid Diagnostic Tool. J. clin. micro. 46, 3987–3996.
Kagambègaab AA, Martikainena TA, Lienemanna AM, Siitonena AN, Traoréb NA, Barrob KA, Haukkaa QA. 2012. Diarrheagenic Escherichia coli detected by 16-plex PCR in raw meat and beef intestines sold at local markets in Ouagadougou, Burkina Faso. Inter. J. Food Micro. 153(1), 154-158.
Kim DA, Kim SA, Bae HM, Jung YM, Lee KA, Seo YN, Lee QA. 2022. Comparison of antimicrobial resistance and molecular characterization of Escherichia coli isolates from layer breeder farms in Korea. Poultry Science. 101(1), 1-7.
Kim YB, Seo JB, Shim SA, Son EB, Noh YJ, Lee KW. 2019. Molecular characterization of antimicrobial-resistant Enterococcus faecalis and Enterococcus faecium isolated from layer parent stock. Poult. Sci. 98, 5892-5899.
Kitchel JK, Rasheed BA, Patel JB. 009. Molecular epidemiology of KPC-producing Klebsiella pneumoniae isolates in the United States: clonal expansion of multilocus sequence type 258, Antimicro. Agents and Chemotherapy 53(8), 3365–3370.
Laarem MA, Barguigua KA, Nayme AM, Akila KA, Zerouali NW, El Mdaghri AB. 2017. Occurrence of plasmid-mediated quinolone resistance and virulence genes in avian Escherichia coli isolates from Algeria. J. Infect. Dev. Ctries. 11, 143–51.
Lee GY, Jang IG, Hwang MS, Rhee HI. 2009. Prevalence and classification of pathogenic Escherichia coli isolated from fresh beef, poultry, and pork in Korea. Int. J. Food Microbiol. 134, 196–200.
Medina AM, Horcajo SA, Jurado RW, De la Fuente JA, Ruiz-Santa-Quiteria GA, Dominguez-Bernal BS. 2011. Phenotypic and genotypic characterization of antimicrobial resistance in enterohemorrhagic Escherichia coli and atypical enteropathogenic E. coli strains from ruminants. J. Vet. Diagn. Investig. 23, 91–5.
Moawad HA, Hotzel AW, Omnia AS. 2017. Occurrence of Salmonella enterica and Escherichia coli in raw chicken and beef meat in northern Egypt and dissemination of their antibiotic resistance markers. Gut Pathogens 9 (1), 57-66.
Momtaz HA, Dehkordi MJ, Hosseini MA, Sarshar MS, Heidari FS. 2013. Serogroups, virulence genes and antibiotic resistance in Shiga toxin-producing Escherichia coli isolated from diarrheic and non-diarrheic pediatric patients in Iran. Gut Pathog. 5, 1757–4749.
Navajas-Benito CA, Alonso VA, Sanz SA. 2017. A. Molecular characterization of antibiotic resistance in Escherichia coli strains from a dairy cattle farm and its surroundings. J. Sci. Food and Agri. 97 (1), 362–365.
Rasmussen MM, Opintan NA, Frimodt-Moller BA, Styrishave JA. 2015. Betalactamase producing Escherichia coli isolates in imported and locally produced chicken meat from Ghana. PLoS ON 10, e0139706
Shobrak MY, Abo-Amer AE. 2014. Role of wild birds as carriers of multi-drug resistant Escherichia coli and Escherichia vulneris. Braz. J. Microbiol. 45(4), 1199–1209.
Valat CA, Forest MA, Billet KS. 2016. Absence of co-localization between pathovar-associated virulence factors and extended-spectrum β-lactamase (blaCTX-M) genes on a single plasmid. Vet. Micro. 192, 163–166.
Vuthy YA, Lay HS, Seiha AA, Kerleguer AS, Aidara-Kane KS. 2017. Antibiotic susceptibility and molecular characterization of resistance genes among Escherichia coli and among Salmonella subsp. in chicken food chains. Asian Pac. J. Trop. Biomed. 7(7), 670–674.
Wang SA, Meng JM, Dai XA, Han YS, Han CW, Ding QA. 2014. Development of an allele-specifc PCR assay for simultaneous sero-typing of avian pathogenic Escherichia coli predominant O1, O2, O18 and O78 strains. PLoS ONE 9, e96904.
White CJ, McIver PA, Deng WA, Rawlinson YM. 2000. Characterisation of two new gene cassettes, aadA5 and dfrA17. FEMS Micro. Letters 182(2), 265–269.
Wright GD. 2010. Antibiotic resistance in the environment: a link to the clinic? Curr. Opi. Micro., 13(5), 589–594.
Yoon SA, Kim KW, Seo JS, Ha EB, Noh YJ, Lee YB. 2020. Characteristics of linezolid-resistant Enterococcus faecalis isolates from broiler breeder farms. Poult. Sci. 99, 6055-6061.
Zhao SA, Blickenstaf SS, Bodeis-Jones PF, Gaines SA, Tong AE, McDermott QW. 2002. Comparison of the prevalence and antimicrobial resistances of Escherichia coli isolates from different retail meats in the United States, 2002 to 2008. Appl. Environ. Microbiol. 78, 1701–7.
Nagwa T. Elsharawy, Hind A. Al-Zahrani (2024), Molecular characterization of Escherichia coli resistance genes in chicken meat; IJB, V24, N6, June, P66-73
https://innspub.net/molecular-characterization-of-escherichia-coli-resistance-genes-in-chicken-meat/
Copyright © 2024
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0