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Molecular cloning and expression profiling of a chalcone synthase gene from Ginkgo biloba, Scutellaria viscidula Bunge and Tree peony (Paeonia suffruticosa)

Manaf Almatar

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Int. J. Biosci.3(10), 187-202, October 2013

DOI: http://dx.doi.org/10.12692/ijb/3.10.187-202

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Abstract

Chalcone synthase (CHS, EC 2.3.1.74) is a key enzyme of the flavonoid/isoflavonoid biosynthesis pathway. In this review, the characterization and expression profile of CHS was investigated from Ginkgo biloba, Scutellaria viscidula, and Tree peony (Paeonia suffruticosa) because of the CHS role in protecting the plants from harmful effects by synthesizing flavonoid compounds. CHS was isolated and characterized from the same species which was described previously. The cDNA sequence of the CHS gene in Ginkgo biloba was 1608 bp with poly (A) tailing, from Scutellaria viscidula1649 bp, and from Tree peony (Paeonia suffruticosa) 1475 bp. cDNA sequence of the CHS gene in G. biloba contained a 1173 bp open reading frame (ORF) encoding a 391 amino acid protein, from Scutellaria viscidula 1170 bp encoding a 390 amino acid protein, and from Tree peony (Paeonia suffruticosa) 1185bp encoding a 394 amino acid protein. Sequence alignment and phylogenetic analysis showed that GbCHS, SvCHS and Ps-CHS1 shared high homology from other plants. Active site of the CoA binding, coumaroyl pocket and cyclization pocket in CHS protein of Medicago sativa were also found in GbCHS, and SvCHS. Likewise, Ps-CHS1 has all the conserved active sites for the CHS function as well as the family signature. The homology-based structural modeling showed that GbCHS most closely resembled of M.sativa (MsCHS2). Real-time (PCR) analysis indicated that GbCHS and Ps-CHS1 had varying expression in different tissues, while expression of SvCHS was induced by MeJA and was the greatest at 12 h. To our knowledge, this is the first report which explains the molecular cloning and expression profiling of a chalcone synthase gene from those species mentioned above.

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Molecular cloning and expression profiling of a chalcone synthase gene from Ginkgo biloba, Scutellaria viscidula Bunge and Tree peony (Paeonia suffruticosa)

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