Morphogenetic diversity of Colletotrichum species infecting Sorghum bicolor in the lake basin regions of Kenya
Paper Details
Morphogenetic diversity of Colletotrichum species infecting Sorghum bicolor in the lake basin regions of Kenya
Abstract
C. sublineolum is among the most destructive sorghum pathogens causing anthracnose in Kenya. Both preventive and breeding for resistance approaches have been developed. However, its continuous evolution into new strains is still a threat to sorghum production especially in tropics and sub-tropics. The pathogen is confirmed to be genetically and morphologically diverse but such information is lacking in Kenya and there is no known number of strains identified to cause the disease in Kenya. Systematic random and biased sampling was conducted in two districts within Lake Basin region where most sorghum is grown to collect anthracnose infected stem, leaf and panicle tissues only. Ten farms separated by four kilometers each were surveyed per district. Ten isolates were obtained per tissue per site, giving a total of sixty isolates for the whole study. Identification of the isolates was done using previous descriptions of the fungus and genetic diversity assessment was performed using simple sequence repeat (SSR) marker. A total of 15 morphologically diverse isolates were isolated from leaf, stem and panicle tissues. Leaf isolates were more diverse followed those infecting the panicle tissues. The stem was frequently infected by isolates resembling C. gloeosporioides. Genetic diversity assessment indicated the presence of two major sub-species with one sub-species showing low stability through evolution into two sub-groups. Only one universal primer amplified the bands thus there is need to involve several other primers to confirm these findings. This study also recommends Koch’s postulate on C. gloeosporioides to ascertain its host range.
Bardas GA, Klonari KT, Koutita O. 2008. Molecular Diversity and Assessment of Biological Characteristics of Greek Colletotrichum lindemuthianum Populations. . Phytopathology 157, 311–319.
CRF. 2011. Plant Pathology working protocol, Nairobi, Kenya.
Cai L, Hyde KD, Taylor PWJ, Weir BS, Waller J, Abang MM, Zhang JZ, Yang YL, Phoulivong S, Liu ZY, Prihastuti H, Shivas RG, McKenzie EHC, Johnston PR. 2009. A polyphasic approach for studying Colletotrichum. Fungal Diversity 39, 183-204.
Chala A, Tronsmo AM, M.B. Brurberg. (2010) Genetic differentiation and gene flow in Colletotrichum sublineolum in Ethiopia, the center of origin and diversity of sorghum, as revealed by AFLP analysis. Plant Pathology 60 (3), 474 – 482.
Crouch JA, Inguagiato JC. 2009. First report of anthracnose disease of ornamental feather reed grass (Calamagrostis x acutifolia ‘Karl Foerster’) caused by Colletotrichum cereale. Plant Disease 93, 203-213.
Crouch JA, Beirn LA. 2009. Anthracnose of cereals and grasses. Fungal Diversity 39, 19-44.
Crouch JA, Clarke BB, Hillman BI. 2006. Unraveling evolutionary relationships among the divergent lineages of Colletotrichum causing anthracnose disease in turfgrass and corn. Phytopathology 96, 46 – 50.
Crouch JA, Glasheen BM, Giunta MA. 2008. The evolution of transposon repeat-induced point mutation in the genome of Colletotrichum cereale: reconciling sex, recombination and homoplasy in an ”asexual” pathogen. Fungal Genetics and Biology 45, 190-206.
Du M, Schardl CL, Nuckles EM, Vaillancourt LJ. 2005. Using mating-type gene sequences for improved phylogenetic resolution of Collectotrichum species complexes. Mycologia 97(3), 641 – 658.
Erpelding JE. 2010a. Field assessment of Anthracnose Disease Response for the Sorghum Germplasm collection from Mopti Region. Am. J. Agr. and Biol. Sci. 5(3), 363-369.
Erpelding JE. 2010b. Anthracnose Disease response in Burundi Sorghum Germplasm Collection. Agric. Biol. J. N. Am 1(6), 1119-1125.
Erpelding JE. 2011. Anthracnose disease Evaluation of Sorghum Germplasm from Honduras. Geneconserve 10 (39), 42-50.
Marley PS, Thakur RP, Ajayi O. 2001. Variation among foliar isolates of Colletotrichum sublineolum of sorghum in Nigeria. Field Crop Research 69, 133-142.
Martel C, Réjasse A, Rousset F, Bethenod MT, Bourguet D. 2003. Host-plant-associated genetic differentiation in Northern French populations of the European corn borer. Heredity 90, 141-149.
Mathur K, Thakur RP, Neya A, Marley PS, Casela CR, Rosewich LU. 2003. Sorghum Anthracnose – Problem and Management Strategies. In: Global 2000: Sorghum and Pearl Millet Diseases 3, 2000, Guanajuato. Proceedings. Sorghum and Millet International Research. Intsormil, In Press.
Mathur SB, Singh K, Hansen JK. 1989. A working Manual on Some Seed-borne Fungal Diseases. Danish Government Institute of Seed Pathology for Developing Countries, Ryvangs Alle 78, DK-2900 Hellerup, Denmark.
Meirelles LDP, Zanette GF, Nobrega GMA. 2009. Morphogenetic characterization of Colletotrichum sublineolum strains, causal agent of anthracnose of sorghum in Brazil, Africa. Tropical Plant Pathology. 34(3), 146 -151.
Mukuru SZ. 1993. Sorghum and millets in eastern Africa: Sorghum and Millets Commodities and Research Environment. Byth, D. E. ed. ICRISAT, Patancheru, India. p. 55-62.
Ngugi HK, King SB, Abayo GO, Reddy YVR. 2002. Prevalence, incidence and severity of sorghum diseases in Western Kenya. Plant Disease 86, 65 – 70.
Pande RP, Thakur RP, Karunakar RI, Bandyopadhyay R, Reddy BVS. 1998. Development of screening methods and identification of stable resistance to anthracnose on sorghum., Amsterdam Field Crop Research 38, 157 -166.
Scott PE, Nosil P, Funk DJ. 2008. Selection and Genomic Differentiation During Ecological Spaciation: Isolating the Contributions of Host Association via a Competative Genome Scan fo Neochlamisus bebbianae Leaf Beetles. Evolution 62 (5), 1162–1181.
Shenoy BD, Jeewon R, Lam WH, Bhat DJ, Than PP, Taylor PWJ, Hyde KD. 2007. Morpho-molecular characterization and epitypification of Colletotrichum capsici (Glomerellaceae, Sordariomycetes), the causative agent of anthracnose in chilli. Fungal Diversity 27, 197-211.
Thakur RP, Mathur K. 2000. Anthracnose. In: Compendium of Sorghum Diseases. (R.A. Frederiksen and G.N. Odvody, Eds). The American Phytopathological Society, St. Paul, MN, USA. p. 10-12.
Zanette GF, Gisele MAN. 2009. Morphogenetic characterization of Colletotrichum sublineolum strains, causal agent of anthracnose of sorghum. Tropical Plant Pathology 34(3), 146-151.
Javan Omondi Were, Julius Onyango Ochuodho (2012), Morphogenetic diversity of Colletotrichum species infecting Sorghum bicolor in the lake basin regions of Kenya; IJAAR, V2, N11, November, P1-7
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