Welcome to International Network for Natural Sciences | INNSpub

Paper Details

Research Paper | October 9, 2022

| Download 16

Multiple insecticide resistance in Aedes aegypti populations from three different setting zones in Benin

Sanoussi Falilath, Yadouleton Anges, Badou Yvette, Hounkanrin Gildas, Tchibozo Carine, Agbanrin Ramziyath, Adewumi Praise, Baba-Moussa Lamine

Key Words:

Int. J. Biosci.21(4), 61-70, October 2022

DOI: http://dx.doi.org/10.12692/ijb/21.4.61-70


IJB 2022 [Generate Certificate]


Multiple insecticide resistance in Aedes aegypti populations from three different setting zones (Dandji, Awaya and Kaoura) in Benin was evaluated from July-October 2021 where firstly adult females aged to 2-5 were subjected to susceptible test using impregnated papers (Permethrin 0. 25%; deltamethrin 0.03%; DDT 4%, and bendiocarb 0.1%) following WHO testing protocol. Moreover, biochemical analysis was done in order to detect Mixed Function Oxydase (MFO), non-specific esterase (NSE) and glutathione-S-transferases (GST) activity in individual 5 days old adult Ae. aegypti that had been reared from larvae and not previously exposed to insecticides. This research showed that  the wild populations of Ae. aegypti populations from all the study sites were fully susceptible to bendiocarb (100% of mortality). However, we noticed that all the populations of Ae. aegypti had developed a strong resistance to DDT with average mortalities of 16%; 20% and 26% in Dandji, Awaya and Kaoura sites respectively. Moderate resistance profiles were recorded when these mosquitoes were exposed to permethrin with average mortalities of 48% ; 54% and 62% respectively in Dandji, Awaya and Kaoura sites. For deltamethrin, only populations of Ae. aegypti from Kaoura were fully susceptible to this insecticide. However, 78% and 84% average mortalities were recorded respectively in Dandji and Awaya. Enzymatic activities (Glutathione-s-transferase (GST) and P450 monooxygenase) in the wild population of Ae. aegypti were significantly higher than the control strain SBE (P < 0,05).   This study provides clear evidence that there is a multiple insecticide resistance in the three wild populations of  Ae. aegypti populations from our study sites. This will jeopardise the successful of the control of  Ae. aegypti in these districts, however, the susceptibility results of the three populations to bendiocarb shows that this insecticide appears to be a good candidate to control these wild populations in case of outbreak of dengue fever.


Copyright © 2022
By Authors and International Network for
Natural Sciences (INNSPUB)
This article is published under the terms of the Creative
Commons Attribution Liscense 4.0

Multiple insecticide resistance in Aedes aegypti populations from three different setting zones in Benin

Weetman D, Kamgang B, Badolo A, Moyes CL, Shearer FM, Coulibaly M. 2018. Aedes mosquitoes and Aedes-borne arboviruses in Africa: current and future threats. Int J Environ Res Public Health 15, 220.

Moyes CL, Vontas J, Martins AJ, Ng LC, Koou SY, Dusfour I, Raghavendra K, Pinto J, Corbel V, David JP. 2017. Contemporary status of insecticide resistance in the major Aedes vectors of arboviruses infecting humans. PLOS Neglected Tropical Diseases 11, e0005625.

Kraemer MU, Sinka ME, Duda KA, Mylne AQ, Shearer FM, Barker CM, Moore CG, Carvalho RG, Coelho GE, Van Bortel W. 2015. The global distribution of the arbovirus vectors Aedes aegypti and Ae. albopictuseLife 4, e08347.

WHO. 2016. Monitoring and managing insecticide resistance in Aedes mosquito populations. Interim guidance for entomologists: World Health Organization.

Fried JR, Gibbons RV, Kalayanarooj S, Thomas SJ, Srikiatkhachorn A, Yoon IK, Jarman RG, Green S, Rothman AL, Cummings DA. 2010.  Serotype-specific differences in the risk of dengue hemorrhagic fever: an analysis of data collected in Bangkok, Thailand from 1994 to 2006. PLOS Neglected Tropical Diseases 4, e617.

Abe H, Ushijima Y, Loembe MM, Bikangui R, Nguema-Ondo G, Mpingabo PI. 2020. Re-emergence of dengue virus serotype 3 infections in Gabon in 2016–2017, and evidence for the risk of repeated dengue virus infections. International Journal of Infectious Diseases 91(3), 129–36.

Diakarida Fofana, Jean Michel Vianney Beugré, Genevieve Lydie Yao-Acapovi, and Sevidzem Silas Lendzele. 2019. Risk of Dengue Transmission in Cocody Abidjan, Ivory Coast). Journal of Parasitology Research 2, 49-54.

Fagbami AH, Onoja AB.  2018. Dengue haemorrhagic fever: An emerging disease in Nigeria, West Africa. Journal of Infection and Public Health 11(6), 757–762.

Gaye A, Ndiaye T, Sy M. 2021. Genomic investigation of a dengue virus outbreak in Thiès, Senegal, in 2018. Scientific Reports 11, 10321.

Ouédraogo S, Benmarhnia T, Bonnet E, Somé P-A, Barro AS, Kafando Y. 2018. Evaluation of effectiveness of a community-based intervention for control of dengue virus vector, Ouagadougou, Burkina Faso. Emerging Infectious Diseases 24, 1859–1867.

Anges Yadouleton, Ramziyath Agbanrin C. Vodounon G, padonou Badirou K. 2014. Seasonal distribution of Aedes aegypti in southern Benin: a risk of dengue virus transmission to urban populations.  International Journal of Innovation and Applied Studies 9(2), 648–654.

Ujiie Moi ML, Kobayashi T, Takeshita N, Kato Y, Takasaki T, Kanagawa S. 2012. Dengue virus type-3 infection in a traveler returning from Benin to Japan. Journal of Travel Medicine’s 19, 255-7.

World Health Organization. 2021. Global strategy for dengue prevention and control 2012–2020. 2021.

Yougang AP, Kamgang B, Bahun, TAW, Tedjou AN, Nguiffo-Nguete D, Njiokou F. 2020. First detection of F1534C knockdown resistance mutation in Aedes aegypti (Diptera: Culicidae) from Cameroon. Infectious Diseases of Poverty 9, 152.

World Health Organization. 2016. Test procedures for insecticide resistance monitoring in malaria vector mosquitoes, 2nd ed. 48p. http://www.who.int/iris/handle/10665/250677.

Ridde V, Carabali M, Ly A, Druetz T, Kouanda S, Bonnet E. 2014. The need for more research and public health interventions on dengue Fever in Burkina Faso. PLOS Neglected Tropical Diseases 8, e2859. http://dx.doi.org/10.1371/journal.pntd.0002859

Penilla RP, Rodriguez AD, Hemingway J, Torres JL, Arredondo-Jimenez JI, Rodriguez MH. 1998. Resistance management strategies in malaria vector mosquito control. Baseline data for a large-scale field trial against Anopheles albimanus in Mexico. Med Vet Entomol 12(1), 217–233.

David JP, Ismail HM, Chandor Proust A, Paine  MJ.  2013. Role of cytochrome P450s in insecticide resistance: Impact on the control of mosquito-borne diseases and use of insecticides on Earth. Philosophical Transactions of the Royal Society B: Biological Sciences 3681612), 201-204.

Namountougou M, Simard F, Baldet T, Diabaté A, Ouédraogo JB, Martin T. 2012. Multiple Insecticide Resistance in Anopheles gambiae s.l. Populations from Burkina Faso, West Africa. PLoS ONE 7(11), e48412.

Finney DJ. 1971. Probit analysis. Cambridge University Press Cambridge.

Akogbéto M, Djouaka R, Noukpo H. 2005. Use of agricultural insecticides in Benin. Bulletin de la Société de Pathologie Exotique 98, 400–405.

Ponlawat J, Scott G, Harrington LC. 2005. Insecticide susceptibility of Aedes aegypti and Aedes albopictus across Thailand. Journal of Medical Entomology 42, 821-825.

Toé HK, Zongo S, Guelbeogo MW, Kamgang B, Viana M, Tapsoba M. 2022. Multiple insecticide resistance and first evidence of V410L kdr mutation in Aedes (Stegomyia) aegypti (Linnaeus) from Burkina Faso. Medical and Veterinary Entomology 36(3), 309–319.


Style Switcher

Select Layout
Chose Color
Chose Pattren
Chose Background