Multivariate analysis of forage sorghum [Sorghum bicolor (L.) Moench] lines for salinity tolerance at germination stage

Paper Details

Research Paper 01/09/2013
Views (368) Download (6)
current_issue_feature_image
publication_file

Multivariate analysis of forage sorghum [Sorghum bicolor (L.) Moench] lines for salinity tolerance at germination stage

Zahra Khodarahmpour
Int. J. Biosci.3( 9), 8-15, September 2013.
Certificate: IJB 2013 [Generate Certificate]

Abstract

This study was conducted to evaluate the effect of salinity on the germination and early growth of forage sorghum lines. In order to an experiment was performed as factorial form under completely randomized design (CRD) with 3 replications. Line factor was contains of 15 lines and 5 levels of salinity (control, -3, -6, -9 and -12 bar) with NaCl. Results of correlation showed that seedling length had the most positive and significant correlation with radicle length (r=0.99**). Factor analysis based on principal component analysis showed that three independent factors 79% of all variation data determined. The first component named germination percentage, seedling length and index seed vigour. The second component named germination characteristics and dry/wet weight ratio of seedling. The three component named mean germination time, plumule length and dry weight of seedling. Cluster analysis (Ward’s minimum variance method) and bipot arranged lines into two groups. Based biplot, lines of KFS2, KFS6, KFS7, KFS8, KFS9, KFS10 and KFS11 were low sensitive to salinity stress. The lines KFS1, KFS12, KFS13, KFS15, KFS16, KFS17 and KFS18 were high sensitive to salinity stress.

VIEWS 7

Ashraf-Jafari A, Zeiaei-Nasab M. 2004. Evaluation  of  genetic  diversity  of  forage  and  seed yield and morphological traits in (Trifolium repens L.) via multivariate analysis. Research Genetic and Breeding of Reglands and Iranian Forests 12, 143-61 (in Persian).

Azhar FM, McNeilly T. 1988. The genetic basis of variation for salt tolerance in Sorghum bicolor (L) Moench seedlings. Plant Breeding 101, 114–121. http://dx.doi.org/10.1111/j.1439-0523.1988.tb00575.x

Basafa M, Taherian M. 2010. Evaluation of drought tolerance in alfalfa (Medicago sativa L.) ecotypes using drought tolerance indices. Environmental Stresses in Crop Sciences 3(1), 69-81 (in Persian).

Bibi A, Sadaqat HA, Tahir MHN, Akram HM. 2012. Screening of sorghum (Sorghum bicolor Var Monech) for drought tolerance at seedling stage in polyethylene Glycol. The Journal of Animal & Plant Sciences 22(3), 671-678.

Bibi A, Sadaqat HA, Akram HM, Mohammed MI. 2010 Physiological markers for screening sorghum (Sorghum bicolor) germplasm under water stress condition. International Journal of Agricultural Biology 12, 451–455.

Chauhan RR, Chaudhary R, Sing, A, Singh PK. 2012. Salt tolerance of Sorghum bicolor cultivars during germination and seedling growth. Research Journal of Recent Sciences 1(3), 1-10.

Hassanein AM. 1985. Salt tolerance of fifty grain sorghum genotypes at seedling stage. Annals of Agricultural Sciences (Egypt) 30,163–176.

ISTA (International Seed Testing Association) 1996. International rules for seed testing rules. Seed Science and Technology 24, (Supplement.) 155-202.

Janmohammadi M, Moradi Dezfuli P, Sharifzadeh F. 2008. Seed invigoration techniques to improve germination and early growth of inbred line of maize under salinity and drought stress. Genetic Applied Plant Physiology 34, 215-26.

Khalesro SH, Aghaalikhani M. 2007. Effect of salinity and water deficit stress on seed germination. Research and Creativity 77, 153-63 (in Persian).

Khodarahmpour Z, Soltani A. 2013. Selection of alfalfa (Medicago sativa L.) cultivars for drought stress tolerance through germination indices. Research on Crops 14(1), 296-303.

Krishnamurthy L, Serraj R, Tom Hash C, Dakheel AJ, Reddy BVS. 2007. Screening sorghum genotypes for salinity tolerant biomass production. Euphytica 156, 15-24.

Maiti RK, De La Rosa-Ibarra M, Sandowal ND. 1994. Genotypic variability in glossy sorghum lines for resistance to drought, salinity and temperature-stress at seedling stage. Journal of Plant Physiology 143, 241–244.

Martinez-Calvo  J,  Gisbert  AD,  Alamar  MC, Hernandorena R, Romero C, Liacer G, Badenes ML. 2008. Study of a germplasm collection of loquat (Eriobotrya japonica Lindl.) by multivariate  analysis.  Genetic  Resources  and  Crop Evolution 55(5), 695-703. http://dx.doi.org/10.1007/s10722-007-9276-8

Munns R. 2005. Genes and salt tolerance: bringing them together. New Phytology 167, 645-63. http://dx.doi.org/10.1111/j.1469-8137.2005.01487.x

Murillo-Amador B, Lopez-Aguilar R, Kaya C, Larrinaga-Mayoral J, Flores, HA. 2002. Comparative effect of NaCl and PEG on germination emergence and seedling growth of cowpea. Journal of Agronomy and Crop Sciences 188, 235-47.

Scasta, JD. 2012. Evaluating alfalfa (Medicago sativa L.) cultivars for salt tolerance using laboratory, greenhouse and field methods. Journal of Agricultural Sciences 4(6), 90-103. http://dx.doi.org/10.5539/jas.v4n6p90

Soltani A, Khodarahmpour Z, Ashraf Jafari A, Nakhjavan SH. 2012. Selection of alfalfa (Medicago sativa L.) cultivars for salt stress tolerance using germination indices. African Journal of Biotechnology 11(31), 7899-7905.

Taylor RM, Young EF, Rivera RL. 1975.  Salt tolerance in cultivars of grain sorghum. Crop Science15, 734–735.

Zaheri A, Bahraminejad S. 2012. Assessment of drought tolerance in oat (Avena sativa) genotypes. Annals of Biological Research 3(5), 2194-2201.