NAC transcription factor family members are differentially expressed in rice seedlings in response to Rice Tungro Spherical Virus
Paper Details
NAC transcription factor family members are differentially expressed in rice seedlings in response to Rice Tungro Spherical Virus
Abstract
The NAC genes encode plant-specific transcriptional factors known to play diverse roles in various plant developmental processes. Rice cultivar Taichung Native 1 (TN1) is susceptible to rice tungro spherical virus (RTSV). Backcross line TW16 was developed by TN1 and RTSV-resistant cultivar UtriMerah. Propagation of RTSV is supported in TN1 but not in TW16, although both TN1 and TW16 remain asymptomatic. Here, we compared the gene expression profiles of TN1 and TW16 infected by RTSV to identify the OsNAC gene expression patterns associated with the accumulation and suppression of RTSV. Among 112OsNAC genes examined by a microarray, approximately 31% and 55% of them were found differentially expressed by RTSV in TN1 and TW16, respectively. Four NAC genes showed continuously expression in TW16 at all days post-inoculation (dpi). Six NAC genes showed higher expression in the TW16 than the TN1 at different dpi with RTSV. Most of the genes in subgroups ONAC2 (50%), ONAC3 (56%), and ONAC7 (80%) were more highly activated in TW16 than in TN1 after RTSV infection. These results suggested that OsNAC genes might be related to the responses induced by the RTSV infection. Whereas, a number of genes in the NAC subgroups NAC1 and were ONAC1 down-regulated for RTSV infection. These OsNAC genes might be associated to the health stage maintenance of the host plants, which may help to clarify the function of these key genes in network pathways.
Aida M, Ishida T, Fukaki H, Fujisawa H, Tasaka M. 1997. Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant. Plant Cell 9, 841–857.
Diaz-Pendo JA, Ding SW. 2008. Direct and indirect roles of viral suppressors of RNA silencing in pathogenesis. Annu. Rev. Phytopathol. 46, 303–326.
Encabo JR, Cabauatan PQ, Cabunagan RC, Satoh K, Lee JH. Kwak DY, De Leon TB, Macalalad RJ, Kondoh H, Kikuchi S, Choi IR. 2009. Suppression of two tungro viruses in rice by separable traits originating from cultivar UtriMerah. Mol. Plant Microbe. Interact. 22, 1268–1281.
Eulgem T, Rushton PJ, Robatzekand S, Somssich IE. 2000. The WRKY super family of plant transcription factors. Trends. Plant Sci. 5, 199-206.
Goda H, Sawa S, Asami T, Fujioka S, Shimada Y, Yoshida S. 2004. Comprehensive comparison of auxin-regulated and brassinosteroid-regulated genes in Arabidopsis. Plant Physiol. 134, 1555-73.
Hibino H. 1983. Relations of rice tungro bacilliform and spherical viruses with their vector Nephotettixvirescens. Ann. Phytopathol. Soc. Jpn. 49, 545–553.
Hull R. 1996. Molecular biology of rice tungro viruses. Annu. Rev. Phytopathol 34, 275–297.
Lee JH, Muhsin M, Atienza GA, Kwak DY, Kim SM, De Leon TB, Angeles E, Coloquio E, Kondoh H, Satoh K, Cabunagan RC, Cabauatan PQ, Kikuchi S, Leung H, Choi IR. 2010. Single nucleotide polymorphisms in a gene for translation initiation factor (eIF4G) of rice (Oryza sativa) associated with resistance to Rice tungro spherical virus. Mol. Plant Microbe. Interact. 23, 29–38.
Li H, Zhou SY, Zhao WS, Su SC, Peng YL. 2009. A novel wall-associated receptor-like protein kinase gene, OsWAK1, plays important roles in rice BLAST disease resistance. Plant Mol. Biol. 69, 337–346.
Nuruzzaman M, Sharoni AM, Kikuchi S. 2013. Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants. Front. Microbiol 4, 248.
Nuruzzaman M, Manimekalai R, Sharoni AM, Satoh K, Kondoh H, Ooka H, Kikuchi S. 2010. Genome-wide analysis of NAC transcription factor family in rice. Gene 465, 30–44.
Saeed AI, Bhagabati NK, Braisted JC, Liang W, Sharov V, Howe EA, Li J, Thiagarajan M, White JA, Quackenbush J. 2006. TM4 microarray software suite. Methods Enzymol. 411, 134–193.
Shamir R, Maron-Katz A, Tanay A, Linhart C, Steinfeld I, Sharan R, Shiloh Y, Elkon R. 2005. EXPANDER – an integrative program suite for microarray data analysis. BMC Bioinformatics 6, 232.
Shibata Y, Cabunagan RC, Cabauatan PQ, Choi IR. 2007. Characterization of Oryzarufipogon-derived resistance to tungro disease in rice. Plant Dis. 91, 1386–1391.
Tameling WI, Baulcombe DC. 2007. Physical association of the NB-LRR resistance protein Rx with a Ran GTPase-activating protein is required for extreme resistance to Potato virus X. Plant Cell 19, 1682–1694.
A. M. Sharoni, M. Nuruzzaman, M. M. Rahman, A. K. M. R. Islam, MM. Hossain, M. F. Alam, F. M. A. Haydar, M. A. Rahman, S. Kikuchi, 2017. NAC transcription factor family members are differentially expressed in rice seedlings in response to Rice Tungro Spherical Virus. Int. J. Biosci., 10(3), 15-23.
Copyright © 2017 by the Authors. This article is an open access article and distributed under the terms and conditions of the Creative Commons Attribution 4.0 (CC BY 4.0) license.