Nutritional quality and microbiological evaluation of ensiled guinea grass, Panicum maximum Jacq. (Poales: Poaceae) and mulberry foliage, Morus spp. (Rosales: Moraceae) mixtures

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Research Paper 08/01/2024
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Nutritional quality and microbiological evaluation of ensiled guinea grass, Panicum maximum Jacq. (Poales: Poaceae) and mulberry foliage, Morus spp. (Rosales: Moraceae) mixtures

Ian D. Fontanilla
Int. J. Biosci. 24(1), 159-165, January 2024.
Copyright Statement: Copyright 2024; The Author(s).
License: CC BY-NC 4.0

Abstract

Silage has been proven to be an effective and alternative source of nutrients for livestock and to provide high-quality feeds, especially during the dry season. However, the ratio of ensiling mixed silages of guinea grass (Panicum maximum Jacq.) and mulberry leaves (Morus spp.) needs to be evaluated, given their nutritional value as livestock feeds. Hence, this paper is to determine the best ratio of these forage materials as an alternative feed for livestock. The experiment was laid out using the Completely Randomized Design (CRD) with six treatments: T1 (100% guinea grass), T2 (100% mulberry leaves), T3 (50% GG + 50% ML), T4 (70% GG + 30% ML), T5 (30% GG + 70% ML), and T6 (56% GG + 44% ML) replicated three times. Treatments were evaluated with their crude protein, crude fat, crude fiber, ash, moisture, dry matter, ADF, NDF, lactic acid, pH, yeasts, molds, and total coliforms. Findings show that silage mixed with a 70% GG + 30% ML ratio produced the highest composition of nutrients (ADF, NDF, DM, moisture, ash, crude fiber, and crude fat) and microbial composition (lactic acid), making it the best-mixed silage for livestock. Therefore, the study recommends 70% guinea grass and 30% mulberry foliage silage mixtures due to their high nutritional value and microbial content. Moreover, the materials for silage production are readily available within the community. Therefore, this practice can be adopted by livestock farmers.

Aganga, AA, Tshwenyane S. 2004. Potentials of guinea grass (Panicum maximum) as forage crop in livestock production. Pakistan Journal of Nutrition 3, 1-4. https://doi.org/10.3923/pjn.2004.1.4

Ba NX, Giang VD, Ngoan LD. 2005. Ensiling of mulberry foliage (Morus alba) and the nutritive value of mulberry foliage silage for goats in central Vietnam. Livestock Research for Rural Development 17(2), 1-9.

Barnhart SK, Nadeau EMG. 2008. The Ensiling Process and Additives. Iowa State University, University Extension.

Bureenok S, Namihira T, Kawamoto Y, Nakada T. 2005. Additive effects of fermented juice of epiphytic lactic acid bacteria on the fermentative quality of guinea grass (Panicum maximum Jacq.) silage. Grassland Science 51(3), 243-248. http://dx.doi.org/10.1111/j.1744-697X.2005.00032.x

Bureenok S, Yuangklang C, Vasupen K, Schonewille JT, Kawamoto Y. 2012. The effects of additives in napier grass silages on chemical composition, feed intake, nutrient digestibility and rumen fermentation. Asian-Australasian Journal of Animal Sciences 25(9), 1248. https://doi.org/10.5713/ajas.2012.12081

He L, Zhou W, Wang C, Yang F, Chen X, Zhang Q. 2019. Effect of cellulase and Lactobacillus casei on ensiling characteristics, chemical composition, antioxidant activity, and digestibility of mulberry leaf silage. Journal of Dairy Science 102(11), 9919-9931. https://doi.org/10.3168/jds.2019-16468

Helmenstine AM. 2020. pKa Definition in Chemistry. https://www.thoughtco.com/what-is-pka-in-chemistry-605521

Heuzé V, Tran G. 2020. Guinea grass (Megathyrsus maximus). Feedipedia, a programme by INRAE, CIRAD, AFZ and FAO. https://www.feedipedia.org/node/416 Last updated on September 15, 2020, 15:40

Iqbal S, Younas U, Sirajuddin, Chan KW, Sarfraz RA, Uddin MK. 2012. Proximate composition and antioxidant potential of leaves from three varieties of Mulberry (Morus sp.): a comparative study. International Journal of Molecular Sciences 13(6), 6651-6664. https://doi.org/10.3390/ijms13066651

Khaing KT, Loh TC, Ghizan S, Halim RA, Samsudin AA. 2015. Feed intake, growth performance and digestibility in goats fed whole corn plant silage and Napier grass. Malaysian Journal of Animal Science 18(1), 87-97.

Kung L, Shaver R. 2001. Interpretation and use of silage fermentation analysis reports. Focus on forage 3(13), 1-5.

Kung L. 1998. A review on silage additives and enzymes. In Proceedings of the 59th Minneapolis Nutrition Conference (pp. 121-135).

Lemus R. 2010. Understanding silage making process and utilization. Cooperative Extension Service, Mississippi State University: Starkville, MS, USA, 3.

McDonald P, Henderson AR, Heron SJE. 1991. The biochemistry of silage. Chalcombe publications.

McDonald P, Watson SJ, Whittenbury R. 1966. The principles of ensilage. Zeitschrift für Tierphysiologie Tierernährung und Futtermittelkunde 21(1‐5), 103-109. https://doi.org/10.1111/j.1439-0396.1966.tb00087.x

Muck RE, Nadeau EMG, McAllister TA, Contreras-Govea FE, Santos MC, Kung Jr L. 2018. Silage review: Recent advances and future uses of silage additives. Journal of Dairy Science 101(5), 3980-4000. https://doi.org/10.3168/jds.2017-13839

Nikodinovic-Runic J, Guzik M, Kenny ST, Babu R, Werker A, Connor KE. 2013. Carbon-rich wastes as feedstocks for biodegradable polymer (polyhydroxyalkanoate) production using bacteria. Advances in applied microbiology 84, 139-200. https://doi.org/10.1016/B978-0-12-407673-0.00004-7

Nishino N, Li Y, Wang C, Parvin S. 2012. Effects of wilting and molasses addition on fermentation and bacterial community in guinea grass silage. Letters in Applied Microbiology  54(3), 175-181. https://doi.org/10.1111/j.1472-765X.2011.03191.x

Ojeda F, Montejo I, Pérez G. 2000. Conservation of mulberry as silage. 1. Effect on nitrogenous compounds. FAO Animal Production and Health Paper, 249-260.

Ojeda F, Montejo I, López O. 2006. Estudio de la calidad fermentativa de la morera y la hierba de guinea ensiladas en diferentes proporciones. Pastosy Forrajes 29(2).

Sahoo A. 2018. Silage for climate resilient small ruminant production. Ruminants: The Husbandry, Economic and Health Aspects, 11. http://dx.doi.org/10.5772/intechopen.74667

Khaing KT, Loh TC, Ghizan S, Halim RA, Samsudin AA. 2015. Feed intake, growth performance and digestibility in goats fed whole corn plant silage and Napier grass. Malaysian Journal of Animal Science 18(1), 87-97.

Wang Y, Chen X, Wang C, He L, Zhou W, Yang F, Zhang Q. 2019. The bacterial community and fermentation quality of mulberry (Morus alba) leaf silage with or without Lactobacillus casei and sucrose. Bioresource Technology 293, 122059. https://doi.org/10.1016/j.biortech.2019.122059

Yitbarek MB, Tamir B. 2014. Silage additives. Open Journal of Applied Sciences 2014. http://dx.doi.org/10.4236/ojapps.2014.45026

Zhang YC, Li DX, Wang XK, Lin YL, Zhang Q, Chen XY, Yang FY. 2019. Fermentation quality and aerobic stability of mulberry silage prepared with lactic acid bacteria and propionic acid. Animal Science Journal 90(4), 513-522. https://doi.org/10.1111/asj.13181

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