Optimization of fermentation conditions for the production of α-amylase by Penicillium sp. isolated from forest soil
Paper Details
Optimization of fermentation conditions for the production of α-amylase by Penicillium sp. isolated from forest soil
Abstract
Starch degrading amylolytic enzymes are most important in the field of industrial microbiology and biotechnology with a huge application in food, fermentation, textile and paper industry. A study was conducted to exploit Penicillium sp. for α-amylase production. In the study Penicillium sp. was found to be a promising producer of α-amylase. Different fermentation conditions for the production of α-amylase from Penicillium sp. were optimized. The optimum production was found at initial pH- 7.0, incubation temperature– 300C, carbon source- Starch (1.5%), organic nitrogen source- Peptone (0.6%), inorganic nitrogen source- (NH4)2SO4 (0.02%) and inorganic salt – KH2PO4 (0.3%) respectively. The study suggests that the mold Penicillium sp. showing 55 hours of optimum incubation time has the potentiality to be an economically viable organism for commercial production of α-amylases.
Abdullah R, Nadeem S, Iqtedar M, Kaleem A, Iftikhar T, Naz S. 2017. Influence of growth conditions on enhanced production of alpha amylase from Penicillium species in solid-state fermentation. Indian Journal of Biotechnology 16(3), 426–432.
Ayansina ADV, Adelaja AO, Mohammed SSD. 2017. Characterization of amylase from some Aspergillus and Bacillus species associated with cassava waste peels. Advances in Microbiology 7(4), 280–292. https://doi.org/10.4236/aim.2017.74023.
Balakrishnan M, Jeevarathinam G, Kumar SKS, Muniraj I, Uthandi S. 2021. Optimization and scale-up of α-amylase production by Aspergillus oryzae using solid-state fermentation of edible oil cakes. BMC Biotechnology 21, 33. https://doi.org/10.1186/s12896-021-00686-7.
Binod P, Palkhiwala P, Gaikaiwari R, Nampoothiri KM, Duggal A, Dey K, Pandey A. 2013. Industrial enzymes – present status and future perspectives for India. Journal of Scientific & Industrial Research 72, 271–286.
Das S, Singh S, Sharma V, Soni ML. 2011. Biotechnological applications of industrially important amylase enzyme. International Journal of Pharma and Bio Sciences 2, 486–496.
De Souza PM, Magalhaes PO. 2010. Application of microbial α-amylases in industry: a review. Brazilian Journal of Microbiology 41, 850–861.
Erdal S, Taskin M. 2010. Production of α-amylase by Penicillium expansum MT-1 in solid-state fermentation using waste loquat (Eriobotrya japonica Lindley) kernels as substrate. Romanian Biotechnological Letters 15, 5342–5350.
Far BE, Ahmadi Y, Khosroshahi AY, Dilmaghani A. 2020. Microbial alpha-amylase production: progress, challenges, and perspectives. Advanced Pharmaceutical Bulletin 10(3), 350. https://doi.org/10.34172/apb.2020.043.
Grandview Research. 2024. Alpha-Amylase baking enzyme market size, share & trends analysis report by source (fungi, plant-based), by application (bread, cookies & biscuits), by region (North America, Europe), and segment forecasts, 2024–2030. https://www.grandviewresearch.com/industry-analysis/alpha-amylase-baking-enzyme-market (accessed 2 October 2024).
Gupta R, Gigras P, Mohapatra H, Goswami VK, Chauhan B. 2003. Microbial α-amylases: a biotechnological perspective. Process Biochemistry 38, 1599–1616.
Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Journal of Analytical Chemistry 31, 426–428. https://doi.org/10.1021/ac60147a030.
Mostafa FA, Wehaidy HR, El-Hennawi HM, Mahmoud SA, Sharaf S, Saleh SA. 2024. Statistical optimization of α-amylase production from novel local isolated Bacillus spp. NRC1 and its textile applications. Catalysis Letters 154, 3264–3275. https://doi.org/10.1007/s10562-023-04545-2.
Okpara MO. 2022. Microbial enzymes and their applications in the food industry: a mini-review. Advances in Enzyme Research 10(1), 23–47. https://doi.org/10.4236/aer.2022.101002.
Premalatha A, Vijayalakshmi K, Shanmugavel M, Rajakumar GS. 2023. Optimization of culture conditions for enhanced production of extracellular α‐amylase using solid‐state and submerged fermentation from Aspergillus tamarii MTCC5152. Biotechnology and Applied Biochemistry 70(2), 835–845. https://doi.org/10.1002/bab.2403.
Sindhu R, Suprabha GN, Shashidhar S. 2009. Optimization of process parameters for the production of α-amylase from Penicillium janthinellum (NCIM 4960) under solid-state fermentation. African Journal of Microbiology Research 3(9), 498–503.
Sindhu R, Suprabha GN, Shashidhar S. 2011. Purification and characterization of α-amylase from Penicillium janthinellum (NCIM 4960) and its application in the detergent industry. Biotechnol Bioinf Bioeng 1(1), 25–32.
Singh R, Kim SW, Kumari A, Mehta PK. 2022. An overview of microbial α-amylase and recent biotechnological developments. Current Biotechnology 11(1), 11–26. https://doi.org/10.2174/2211550111666220328141044.
Sivaramakrishnan S, Gangadharan D, Nampoothiri KM, Soccol CR, Pandey A. 2006. Alpha amylases from microbial sources: an overview on recent developments. Food Technology and Biotechnology 44, 173–184.
Tiwari KL, Jadhav SK, Fatima A. 2007. Culture conditions for the production of thermostable amylase by Penicillium rugulosum. Global Journal of Biotechnology and Biochemistry 2, 21–24.
Van der Maarel MJEC, van der Veen B, Uitdehaag JCM, Leemhuis H, Dijkhuizen L. 2002. Properties and applications of starch-converting enzymes of the α-amylase family. Journal of Biotechnology 94, 137–155.
Verified Market Reports. 2024. Global alpha-amylase market by type (plants, bacteria), by application (fruit ripening, medical diagnostics), by geographic scope and forecast. https://www.verifiedmarketreports.com/product/alpha-amylase-market/ (accessed 2 October 2024).
Vidya B, Gomathi D, Kalaiselvi M, Ravikumar G, Uma C. 2012. Production and optimization of amylase from Penicillium chrysogenum under submerged fermentation. World Journal of Pharmaceutical Research 1(4), 1116–1125.
Bhisma Narayan Swargiari, Saranga Ranjan Patgiri (2024), Optimization of fermentation conditions for the production of α-amylase by Penicillium sp. isolated from forest soil; IJB, V25, N5, November, P19-26
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