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Over expression of Jatropha’s dehydrin jcdhn-2 enhances tolerance to water stress in rice plants

Research Paper | August 1, 2018

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Samar A. Omar, Nabil I. Elsheery, Hazem M. Kalaji, Adam Ławicki

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Int. J. Biosci.13( 2), 54-66, August 2018

DOI: http://dx.doi.org/10.12692/ijb/13.2.54-66


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Jatropha curcas’s dehydrin (JC-DHN2) has been previously shown to play a role during natural dehydration process associated with maturation of Jatropha curcas seed. In this study, we generated transformed rice plant (tp) overexpressing Jc-DHN2 gene and examined the role of over expressed gene in improving the drought tolerance. The tp plants showed a stronger growth under water stress condition induced by addition 20% of PEG 6000. It also showed an enhanced water stress tolerance as indicated by growth parameters included, fresh and dry weight, chlorophyll content, maximum quantum yield, actual quantum yield of photosystem II and non-photochemical quenching. Also, tp plants showed higher membrane stability under drought comparing with non-transformed plant (wt) as indicated through determination of membrane electrolyte leakage, the values of malondialdehyde and, hydrogen peroxide content as indicator for oxidation level. The tp plant had higher content of osmoregulators substances such as proline, free amino acids and total soluble sugar. The tp plant showed higher values for enzyme activity such as superoxide dismutase, catalase and ascorbate peroxidase compared with wt. Our results clearly showed that tp rice plant with JcDHN-2 better coped with drought stress due increasing photosynthetic efficiency and antioxidant enzymes activities.


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Over expression of Jatropha’s dehydrin jcdhn-2 enhances tolerance to water stress in rice plants

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