Pharmacological evaluation of a freshwater mollusc, Bellamya bengalensis lamarck on adjuvant induced arthritis in wistar rats
Paper Details
Pharmacological evaluation of a freshwater mollusc, Bellamya bengalensis lamarck on adjuvant induced arthritis in wistar rats
Abstract
Arthritis is a painful muscoskeletal disorder of unknown etiology affecting a large population globally. Non-steroidal antiinflammatory drugs have been used for long but have side effects like gastric ulcer, gastric bleeding, leucopenia etc. Bellamya bengalensis is a freshwater mollusc found abundantly of Viviparidae family was used to treat various ailments like conjunctivitis, arthritis etc. The present study aimed to show the attenuation of inflammation by phosphate buffer saline extract of Bellamya bengalensis Lam (PBB) in Freund’s Complete adjuvant (FCA) induced arthritis model. The arthritis was induced in Wistar rats using FCA. Changes in body weight, paw volume, ankle diameter, arthritic score were studied as parameters of chronic inflammation. Serum cytokine profile and tissue antioxidant profile of the paw was studied at the end of the study. Radiology of the hindlegs and the histopathology of the ankle joints were also studied. The PBB treated group of rats showed normal gain in body weight with reduction in paw volume by 90.23% and 96.27% (p<0.01) at 200mg/kg and 400mg/kg when compared with FCA induced control. The proinflammatory cytokine was reduced by 56.79% in serum in PBB 400 mg/kg and the altered antioxidant parameters in inflamed rat paw were restored back to normal when treated with PBB extracts. Histopathology results revealed fewer inflammatory lesions in PBB treated rats when compared to adjuvant induced control rats. In conclusion, PBB at a dose of 200mg/kg and 400mg/kg, orally for 28 days showed significant reduction in chronic inflammation which may be through TNF-α inhibitory pathway.
Briggs AM, Cross MJ, Hoy DG, Sànchez-Riera L, Blyth FM, Woolf AD. 2016.Musculoskeletal health conditions represent a global threat to healthy aging: a report for the 2015 World Health Organization world report on Ageing and health. Gerontologist 56, S234–55.
Barbour KE, Helmick CG, Boring MA, Brady TJ. 2017. Vital signs: prevalence of doctor-diagnosed arthritis and arthritis-attributable activity limitation — United States, 2013—2015. MMWR. Morbidity and mortality weekly report 66, 246–253. http://dx.doi.org/10.15585/mmwr.mm6609e1
Brennan-Olsen SL, Cook S, Leech MT, Bowe SJ, Kowa lP, Naidoo N. 2017. Prevalence of arthritis according to age, sex and socioeconomic status in six low and middle income countries: Analysis of data from the World Health Organization study on global AGEing and adult health (SAGE) Wave 1. BMC Musculoskeletal Disorders 18, 271. http://dx.doi.org/10.1186/s12891-017-1624-z
Wang Q, Kuang H, Su Y, Sun Y, Feng J, Guo R. 2013. Naturally derived antiinflammatory compounds from Chinese medicinal plants. Journal of Ethnopharmacology 146, 9–39. http://dx.doi.org/10.1016/j.jep.2012.12.013
Billiau A, Matthys P. 2001. Modes of action of Freund’s adjuvants in experimental models of autoimmune diseases. Journal of Leukocyte Biology 70, 849–60. http://dx.doi.org/10.1189/jlb.70.6.849
Kunnumakkara AB, Sailo BL, Banik K, Harsha C, Prasad S, Gupta S, Bharti AC. 2018. Chronic diseases, inflammation, and spices: how are they linked?Journal of translational medicine 16, 14. http://dx.doi.org/10.1186/s12967-018-1381-2
Gor AP, Saksena M. 2011. Adverse drug reactions of nonsteroidal anti-inflammatory drugs in orthopedic patients. Journal of pharmacology & pharmacotherapeutics 2, 26-9. http://dx.doi.org/10.4103/0976-500X.77104
Obiri DD, Osafo N, Oppong-Sarfo J, Prah JK. 2014.Margaritariadiscoidea (Euphorbiaceae) stem bark extract attenuates allergy and Freund’s adjuvant-induced arthritis in rodents. Pharmacognosy Research 6, 163-71 http://dx.doi.org/10.4103/0974-8490.129039
Lindequist U. 2016. Marine-Derived Pharmaceuticals – Challenges and Opportunities. Biomolecules & therapeutics 24, 561-571. http://dx.doi.org/10.4062/biomolther.2016.181
Prabhakar AK, Roy SP. 2009. Ethnomedicinal Uses of Some Shell Fishes by People ofKosi River Basin of North Bihar, India. 2009. Ethnomedicine 3, 1-4.
Liu YL, Lin HM, Zou R, Wu JC, Han R, Raymond LN. 2009. Suppression of complete Freund’s adjuvant-induced adjuvant arthritis by cobratoxin. Acta pharmacologica Sinica 30, 219-27. http://dx.doi.org/10.1038/aps.2008.20
Committee for the Purpose of Control and Supervision on Experiments on Animals. 2003. CPCSEA guidelines for laboratory animal facility. Indian Journal of Pharmacology 35, 257.
Adhikari A, Saha A, Indu R, Sur TK, Das AK. 2017. Evaluation of anti-inflammatory effect of beetroot extract in animal models. International Journalof Basic and Clinical Pharmacology 6, 2853-8. http://dx.doi.org/10.18203/23192003.ijbcp20175207
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.
Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. Methods in Enzymology 52, 302–310. http://dx.doi.org/10.1016/S0076-6879(78)520.32-6
Moshage H, Kok B, Huizenga JR, Jansen PL. 1995. Nitrite and nitrate determinations in plasma: critical evaluation. Clinical Chemistry 41, 892–896.
Lawrence R, Burk R. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and biophysical research communications 71, 952–958.
Patro G, Bhattamisra SK, Mohanty BK, Sahoo HB. 2016. In vitro and In vivo Antioxidant Evaluation and Estimation of Total Phenolic, Flavonoidal Content of Mimosa pudica L. Pharmacognosy Research 8, 22–28. http://dx.doi.org/10.4103/0974-8490.171099
Mahdi HJ, Khan N, Asmawi M, Mahmud R, A/L Murugaiyah V. 2017. In vivo anti-arthritic and anti-nociceptive effects of ethanol extract of Moringa oleifera leaves on complete Freund’s adjuvant (CFA)-induced arthritis in rats, Integrative Medicine Research 7, 85-94. http://dx.doi.org/10.1016/j.imr.2017.11.002
Desai SD, Patil BS, Kanthe PS, Potekar RM. 2015. Effect of ethanolic extract of Terminalia arjuna on liver functions and Histopathology of liver in albino rats fed with hyperlipidemic diet. International Journal of Pharmacy and Pharmaceutical Sciences 7, 302-306.
Abotsi WM, Woode E, Ainooson GK, Amo-Barimah AK, Boakye-Gyasi E. 2010. Antiarthritic and antioxidant effects of the leaf extract of Ficusexasperata P. Beauv. (Moraceae). Pharmacognosy Research 2, 89-97.
Mbiantcha M, Almas J, Shabana SU, Nida D, Aisha F. 2017. Anti-arthritic property of crude extracts of Piptadeniastrumafricanum (Mimosaceae) in complete Freund’s adjuvant-induced arthritis in rats. BMC complementary and alternative medicine 17, 111. http://dx.doi.org/10.1186/s12906-017-1623-5
Ben IO, Woode E, Koffuor GA, Boakye-Gyasi E, Titiloye NA. 2017. Effect of Trichiliamonadelpha (Meliaceae) extracts on bone histomorphology in complete Freund’s adjuvant-induced arthritis. Journal of intercultural ethnopharmacology 6, 177-185. http://dx.doi.org/10.5455/jice.20170218092913
Rajaram C, Ravindra Reddy K, Chandra Sekar KB. 2015. Evaluation of antiarthritic activity of Caeslpiniapulcherrima in freund’s complete adjuvant induced arthritic rat model. Journal of Young Pharmacists 7, 128-132. http://dx.doi.org/10.5530/jyp.2015.2.12
Ray M, Deb N, Datta R, Besra S. 2017. Activity of The Lymph Extracted from Bellamya bengalensis f. annandalei In Rodent Models. World Journal of Pharmaceutical Research 6523, 772-783. http://dx.doi.org/10.20959/Wjpr201712-9683.
Parameswaran N, Patial S. 2010. Tumor necrosis factor-α signaling in macrophages. Critical reviews in eukaryotic gene expression 20, 87-103.
Ray M, Bhunia NS, Bhunia AS, Ray S. 2016. Expression analyses of interferon gamma, tumor necrosis factor alpha and inducible nitric oxide synthase in the hemocyte morphotypes of two commercially important Indian molluscs. Aquaculture Reports 4, 30–35. http://dx.doi.org/10.1016/j.aqrep.2016.05.005
Ali S SK, Maiti S. 2017. Therapeutic Potentials of Edible Freshwater-Snail Bellamya Bengalensis Extract against Arsenic-induced Rat Tissue Damage are Conferred by Antioxidative Mechanism and Attenuation of Pro-Inflammatory Response. International Journal of Biomedical Science 13, 135-146.
Ekambaram S, Perumal SS, Subramanian V. 2010. Evaluation of antiarthritic activity of Strychnos potatorum Linn seeds in Freunds adjuvant induced arthritic rat model. BMC Complementary and Alternative Medicine 10, 56. http://dx.doi.org/10.1186/1472-6882-10-
Sangita Bhattacharya, Anjan Adhikari, Abhijit Chanda, Tapas Kumar Sur (2019), Pharmacological evaluation of a freshwater mollusc, Bellamya bengalensis lamarck on adjuvant induced arthritis in wistar rats; IJB, V14, N2, February, P172-182
https://innspub.net/pharmacological-evaluation-of-a-freshwater-mollusc-bellamya-bengalensis-lamarck-on-adjuvant-induced-arthritis-in-wistar-rats/
Copyright © 2019
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0