Phenol tolerance of bacteria- a case of spontaneous or adaptive mutation?

Paper Details

Research Paper 01/07/2019
Views (606)
current_issue_feature_image
publication_file

Phenol tolerance of bacteria- a case of spontaneous or adaptive mutation?

Lucky Nandi, Nilanjan Maitra, Sanjib Kumar Manna, Ashis Kumar Panigrahi
Int. J. Biosci. 15(1), 110-119, July 2019.
Copyright Statement: Copyright 2019; The Author(s).
License: CC BY-NC 4.0

Abstract

Origin of mutation is a long lasting dilemma that often haunts researchers at their study. The same query comes up at our bacterial phenol degradation study. Phenol that is one of the most toxic, carcinogenic chemicals can be tolerated by some bacteria those can not only tolerate but also use phenol as substrate by enzymatic degradation. However, like other toxic materials, phenol also exhibits substrate inhibition at higher concentration. Primarily to find out the substrate inhibition, we isolated and identified six phenol degrading bacteria (GSI-1 to 6) (Streptococcus urinalis, Pseudomonas sp., Bacillus sp., Staphylococcus epidermidis, Rhodococcus sp. and Micrococcusluteus) from polluted waters of West Bengal, India, and studied their tolerance level at different phenol concentrations (200 mg l-1 to 1500 mg l-1); from the study 1200 mg l-1 phenol concentration was defined as inhibitory concentration. The growth rate study up to the inhibitory phenol concentration (1200 mg l-1) showed higher growth rate of the isolates at lower phenol concentrations (200 – 600 mg l-1) defining substrate inhibition. However, to our surprise in the further study of phenol degradation at that inhibitory concentration, all of these previously acclimatized bacteria were able to nearly completely degrade the 1200 mg l-1 phenol concentration- presumably due to mutation. But question arises as we obtained this same trend for phylogenitically distantly located bacterial strains- that the origin of this mutation is spontaneous or adaptive attributed by the phenol to the organisms to tolerate the inhibitory phenol concentrations.

Autenrieth RL, Bonner JS, Akgerman A, Okaygun M, McCreary, EM. 1991. Biodegradation of phenolic wastes. Journal of Hazardous Materials 28, 29–53.

Busca G, Berardinelli S, Resini C, Arrighi L. 2008. Technologies for the removal of phenol from fluid streams: A short review of recent developments. Journal of Hazardous Materials 160, 265–288.

Cairns J, Overbaugh J, Miller S. 1988. The origin of mutants. Nature 335, 142–145.

Chen CL, Wu JH, Liu WT. 2008. Identification of important microbial populations in the mesophilic and thermophilic phenol degrading methanogenic consortia. Water Research 42, 1963–1976.

Chung K-T, Wong T-Y, Huang Y-W, Lin Y. 1998. Tannins and human health: a review. Critical Reviews in Food Science and Nutrition 38, 421–464.

Kahru A, Maloverjan A, Sillak H, Pollumaa L. 2002. The toxicity and fate of phenolic pollutants in the contaminated soils associated with the oil-shale industry. Environmental Science and Pollution Research 1, 27–33.

Keith LH, Telliand WA. 1979. Priority pollutants. Environmental Science & Technology 13, 416–423.

Lob KC, Tar PP. 2000. Effect of additional carbon Source on biodegradation of phenol.Bulletin of Environmental Contamination and Toxicology 64, 756–767.

Maitra N, Bandopadhyay C, Samanta S, Sarkar K, Sharma AP, Manna SK. 2015. Isolation, identification and efficacy of inorganic phosphate solubilizing bacteria from oxbow lakes of West Bengal, India. Geomicrobiology Journal 23, 751–758.

Molin G, Nilsson I. 1985. Degradation of phenol by Pseudomonas putida ATCC 11172 in continuous culture at different ratios of biofilm surface to culture volume.Applied and Environmental Microbiology 50, 946–950.

Przybulewska K, Wieczorek A, Nowak A. 2005. Isolation of microorganisms capable of styrene degradation.Polish Journal of Environmental Studies 15, 777–783.

Singh S, Singh BB, Chandra R. 2009. Biodegradation of phenol in batch culture by pure and mixed strains of Paenibacillus sp. and Bacillus cereus. Polish Journal of Microbiology 58(4), 319–325.

Sung RH, Soydoa V, Hiroaki O. 2000. Biodegradation by mixed microorganism of granular activated carbon loaded with a mixture of phenols. Biotechnology Letters 22, 1093–1096.

Wei GH, Yu JF, Zhu YH, Chen WM, Wang L. 2008. Characterization of phenol degradation by Rhizobiumsp CCNWTB 701 isolated from Astragaluschrysopteruin mining tailing region. Journal of Hazardous Materials 151, 111–117.

Yang CF, Lee CM. 2007. Enrichment, isolation, and characterization of phenol degrading Pseudomonas resinovoransstrain P-1 and Brevibacillus sp. strain P-6. International Biodeterioration& Biodegradation 59, 206–210.

Yang RD, Humphrey AE. 1975. Dynamic and steady state studies of phenol biodegradation in pure and mixed cultures. Biotechnology and Bioengineering 17, 1211–1235.

Related Articles

Yield performance assessment of different mustard cultivars under field conditions

Md. Khan Jahan Ali, Md. Moshiur Rahman, Kamrun Nahar, Sharmin Ara Jannat, Mst. Khadija Khatun, Sushan Chowhan, Md. Habibur Rahman, Int. J. Biosci. 27(2), 277-282, August 2025.

Species composition of xylomicobiota of some woody plants distributed in Azerbaijan

K. F. Bakhshaliyeva, V. Y. Hasanova, N. R. Namazov, B. N. Aliyeva, S. C. Garayeva, S. E. Nagiyeva, P. Z. Muradov, Int. J. Biosci. 27(2), 267-276, August 2025.

Perception of producers and processors on sorghum diversity in the context of climate change in center and Northern Benin

Parfait Segla Alohoutade1,2,3, Alphonse Sako Avocefohoun*1,2,3, Sènan Vodouhe2, Mohamed Kanazoe1,2,3, Nicodème Chabi1, Lamine Said Baba-Moussa3, Int. J. Biosci. 27(2), 256-266, August 2025.

Implications of aberrant glycosylation on age-related disease progression

Tahmid Ahmad Patwary, Mukramur Rahman, Md. Nafis Fuad Prottoy, Sayad Md. Didarul Alam, Int. J. Biosci. 27(2), 243-255, August 2025.

Epizootic status and eradication of parasitic diseases in brown bears transferred to the rehabilitation center in Azerbaijan

Siala İ. Rustamova, Aygun A. Azizova, Gular R. Mammadova, Ramin S. Mammadov, Int. J. Biosci. 27(2), 236-242, August 2025.

Cytotoxic and apoptotic effects of Annona squamosa (Atis) crude leaf extract against A549 (Human Lung Adenocarcinoma) cell line

Ashton U. Lim, Noricel U. Garcia, Alkauzar H. Tantong, Int. J. Biosci. 27(2), 226-235, August 2025.

Perceptions of stakeholders towards sheep and goat dairy products in Benin

Yvette Adje, Philippe Sessou, Aretas Tonouhewa, Paulin Azokpota, Lamine Baba-Moussa, Souaïbou Farougou, Int. J. Biosci. 27(2), 211-225, August 2025.

Medicinal plants sold in Daloa markets: Traditional knowledge and Public health issues

Kouakou Yao Bertin, Kouakou Assoman Serge Alain, Kouame Yao Anicet Gervais, Malan Djah François, Bakayoko Adama, Int. J. Biosci. 27(2), 200-210, August 2025.