Physiological impact of sodium azide on Helianthus annus seedlings

Paper Details

Research Paper 01/05/2014
Views (427) Download (9)
current_issue_feature_image
publication_file

Physiological impact of sodium azide on Helianthus annus seedlings

S. Elfeky, S. Abo-Hamad, K. M. Saad-Allah
Int. J. Agron. Agri. Res.4( 5), 102-109, May 2014.
Certificate: IJAAR 2014 [Generate Certificate]

Abstract

The present study was carried out to demonstrate the effect of sodium azide on Helianthus annuus var.“Giza 53” at the green house of Botany Department, Faculty of Science, Tanta University, Egypt. Seeds were soaked in different concentrations of sodium azide (0.5, 1.0 and 2.0 mM) for different time intervals (30, 60, 90, 120 and 150 minutes). Control was maintained by soaking the seeds in distilled water only. The increased concentrations of sodium azide and time of soaking had a negative effect on the percentage of germination, shoot height, chla, chlb, lipid peroxidation and catalase activity. Increasing the concentrations and time of soaking of sodium azide stimulated the antioxidant defense of Helianthus annuus by increasing carotenoids, peroxidase activity and protein content. Pattern of protein was changed with increasing concentration and time of soaking.

VIEWS 11

Adamu AK, Clung SS, Abubakar S. 2004. Effect of ionizing radiation (gamma rays) on tomato (Lycopersicon esculentum L.) in Nigeria. Journal of Experimental Biology 5(2), 185- 193.

Adamu AK, Aliyu H. 2007. Morphological effects of sodium azide on tomato (Lycopersicon esculentum Mill.). The Scientific World Journal 2(4), 9-12.

Ahloowalia BS, Maluszynski M. 2001. Induced mutations: a new paradigm in plant breeding. Euphytica 118, 167–173.

Al-Qurainy F, Khan S. 2009. Mutagenic effect of sodium azide and its application in crop improvement. World Applied Science Journal 6, 1589-1601.

Asmahan AMA. 1993. Effect of gamma irradiation and chemical mutagens on gene expression in Zea mays L. PhDThesis. Departmentof Genetics, Faculty of Agriculture, Ain shams University.

Bailly C, Benamar A, Corbineau F. 1996. Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiologya Plantarum 97, 104-110.

Bailly CJ, Leymarie A, Lehner S, Rousseau D, Côme- Corbineau F. 2004. Catalase activity and expression in developing sunflower seeds as related to drying. Journal of Exprimental Botany 55, 475-483.

Beauchamp C, Fridovich I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44, 276-287.

Behera RK, Mishrap CH, Choudhury NK. 2002. High irradiance and water stress induce alterations in pigment composition and chloroplast activities of primary wheat leaves. Journal of Plant Physiology 159, 967-973.

Bradford MM. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248-254.

Broertjes C, van Harten AM. 1988. Shoot apices: Organization and post-irradiation behavior. In: Broertjes C., van Harten AM. (Eds.). Developments in crop science 12, Applied mutation breeding for vegetatively propagated crops. Elsevier 29-44.

Burton GW, Ingold KU. 1984. β- carotene: an unusual type of lipid antioxidant. Science 224, 569-573.

Diedrick TJ, Frish DA, Gengenbach C. 1990. Tissue culture isolation of a second mutant locus for increased Threonine accumulation in Azide-mutated maize. Theoretical and Applied Genetics 75, 209-217.

Ferrat L, Pergent-Martini C, Romeo M. 2003. Assessment of the use of biomarkers in aquatic plants for the evaluation of environmental quality: application to sea grasses. Aquatic Toxicology 65, 187–204.

Gehlot P. 2012. Innfluence of sodium azide on the growth and nodulation in Trigonella foenum graceum. Plant Archives 12 (2), 989- 991.

Kato M, Shimizu S. 1987. Chlorophyll metabolism in higher plants. Chlorophyll degradation in senescing tobacco leaves: phenolic-dependent peroxidative degradation. Canadian Journal of Botany 65, 729-735.

Kelley WD, Rodriguez-Kabana R. 1979. Nematicidal activity of sodium azide. Nematropica 8, 49-51.

Kleinhofs AW, Owais M, Nilan RA. 1978. Azide. Mutation Research 55, 165-195.

Kumar CN, Knowles RN. 1999. Changes in lipid peroxidation and lipolytic and free radical scavenging enzyme activities during ageing and sprouting of potato ( Solanum tuberosum ) seed tubers. Plant Physiology 102, 115-124.

Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.

Lenoir C, Corbineau F, Come D. 1986. Barley (Hordeum vulgare) seed dormancy as related to glumella characteristics. Physiologia Plantarum 68, 301–307.

Mahmoud AA, Al-Twaty N. 2006. Effect of Gamma irradiation and sodium azide on some economic traits in tomato. Saudi Journal of Biological Sciences 13(1), 44-49.

Metzner H, Rau H, Senger H. 1965. Ultersuchungen Zur synchronisierbarkeit einzelner pigment mangel – Mutanten von chlorella. Planta 65,186-194.

Murthy UMN, Kumar PP, Sun WQ. 2003. Lipid peroxidation, sugar hydrolysis, Maillared reactions and their relationship to glass State transition. Journal of Experimental Botany 54(384), 1057-1067.

Ogborn JEA. 1987. Striga control under present farming conditions. In: L.J. Musselman (ed.) Parasitic weeds in agriculture. CRC Press Inc., Boca Raton, Florida. 1, 145-158.

Osama MS. 2002. Molecular genetic studies on irradiated wheat plants. PhDThesis. Department of Genetics, Faculty of Agriculture, Ain shams University.

Pueratas MC, Mc- Carthy I, Del Rio LA. 2002. Plant proteases protein degradation and oxidative stress: Role of peroxisomes. Plant Physiology and Biochemistry 40, 521- 530.

Parker C, Riches CR. 1993. Parasitic weeds of the world: Biology and control, CAB int., Walliford, U.K. 332.

Ramaiah KV. 1987. Breeding cereal grains for resistance to witch weed. In: L.J. Musselman (ed.). Parasitic weeds in Agriculture vol. 1. CRC press, Boca Raton, F.L. 227-242.

Robertson DG, Rodriguez-Kabana R. 2000. Nematological survey of selected soybean and cottonfields in Alaba ma. Proc. Southern Soybean Disease Workers. 11.

Salim K, Al-Quainy F, Anwar F. 2009. Sodium azide: a chemical mutagen for enhancement of agronomic traits of crop plants. An International Journal of Science and Technology 4, 1-21.

Tayefi-Nasrabadi HG, Dehghan B, Daeihassani A, Movafegi- samadi A. 2011. Some biochemical properties of catalase from safflower (Carthamus tinctorius L. cv. M-CC-190). African Journal of Agricultural Research 6(23), 5221-5226.

Uchiyama M, Mihara M. 1978. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Analytical Biochemistry 86, 271-8.

Verma S, Dubey RS. 2003. Lead toxicity induces lipid peroxidation and alter the activities of antioxidant enzymes in growing rice plants. Plant Science 164, 645- 655.

Walters C. 1998. Understanding the mechanisms and kinetics of seed ageing. Seed Science Research 8, 223-224.