Phytochemical screening and determination of antimicrobial activity of different extracts from the roots and leaves of Stylocheaton Hypogaeus, a plant used for the treatment of prostatitis in Senegal
Paper Details
Phytochemical screening and determination of antimicrobial activity of different extracts from the roots and leaves of Stylocheaton Hypogaeus, a plant used for the treatment of prostatitis in Senegal
Abstract
Stylocheaton hypogaeus is a medicinal plant belonging to the Araceae family of African origin. It is widely used traditionally in southern Senegal to treat various diseases, mainly prostatitis, lung cancer and hemorrhoids. The main objective of this study is to evaluate the antibacterial properties of different leaf and root extracts of the Stylocheaton hypogaeus plant. A phytochemical screening test was previously carried out to highlight the presence of certain families of secondary metabolites, generally responsible for the biological activity of plants. For antibacterial activity, the extracts are tested on Gram-positive bacteria Staphylococcus aureus ATCC29213 and Gram-negative Escherichia coli ATCC25922. Thus, standard agar diffusion and macrodilution techniques were used to determine the inhibition diameters, the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The results revealed a very good antibacterial activity of the different extracts of these two organs, with in fact an inhibition diameter of 21.11 mm for the ethyl acetate extract of the roots on the Escherichia coli ATCC25922 strain. The delipidated methanolic extracts of the roots and leaves showed the best antibacterial activity with a MIC of 1.5625 mg/mL, respectively on the Escherichia coli ATCC25922 and Staphylococcus aureus ATCC29213 strains. This remarkable antibacterial activity of the methanolic extract of the roots of Stylocheaton hypogaeus justifies its use in traditional medicine for the treatment of prostatitis, a disease whose infection is generally caused by the bacterium Escherichia coli ATCC25922.
Ponce AG, Fritz R, Del Valle C, Roura SI. 2003., « Antimicrobial activity of essential oils on the native microflora of organic Swiss chard », LWT-Food Science and Technology 36(7), 679‑684 p.
Rechner AR, Kuhnle G, Bremner P, Hubbard GP, Moore KP, Rice-Evans CA. 2002. « The metabolic fate of dietary polyphenols in humans », Free Radical Biology and Medicine 33(2), 220‑235 p.
Bauer AW, Kirby WMM, Sherris JC, Turck M. 1966. « Antibiotic susceptibility testing by a standardized single disk method », American journal of clinical pathology 45(4), ts, 493‑496 p.
Mbow B, Gaye AA, Diop B, Kone AD, Diallo I, Gaye M. 2022. « Optimization of Extraction Parameters, Total Polyphenols and Flavonoids Contents, and Antioxidant Activity of the Aqueous Extract of Vernonia amygdalina Leaves », American Journal of Applied Chemistry 10(6), Art. http://dx.doi.org/10.11648/j.ajac.20221006.12.
Amal BR, Amel M. 2021.. « Contribution à l’évaluation de l’activité antimicrobienne de la plante médicinale Ruta tuberculata forssk. », biologie, juin
NIARE BT. 2007. « Le résidu post mictionnel avant et après adénomectomie transvésicale de la prostate dans le service d’Urologie du CHU de Point. G », PhD Thesis, Thèse Med Bamako,
Bssaibis F, Gmira N, Meziane M. 2009. « Activité antibactérienne de Dittrichia viscosa (L.) W. Greuter », Revue de Microbiologie Industrielle, Santé et environnement (3), 44‑55,
Barak JD, Schroeder BK. 2012. « Interrelationships of Food Safety and Plant Pathology: The Life Cycle of Human Pathogens on Plants », Annual Review of Phytopathology, 50(50), 241‑266 p. sept. http://dx.doi.org/10.1146/annurev-phyto-081211-172936.
Ploy MC, Poyart C, Cattoir V, Denis F, Martin C. 2016. Bactériologie médicale: techniques usuelles. Elsevier Health Sciences.
Gulluce M. 2007. « Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia », Food chemistry 103(4), 1449‑1456,
Cowan MM. 1999. « Plant Products as Antimicrobial Agents », Clin Microbiol Rev, 12(4), 564-582 p, oct. http://dx.doi.org/10.1128/CMR.12.4.564.
Oguntibeju O. 2018. « Medicinal plants with anti-inflammatory activities from selected countries and regions of Africa », JIR, 11, 307‑317, août. http://dx.doi.org/10.2147/JIR.S167789.
Olarewaju OO, Fajinmi OO, Naidoo KK, Arthur GD, Coopoosamy RM. 2022. « A review of the medicinal plants with immune-boosting potential », JOMPED, 6(1), oct. http://dx.doi.org/10.4102/jomped.v6i1.158.
Khan P, Memon MY, Imtiaz M, Aslam M. 2009. « Response of wheat to foliar and soil application of urea at different growth stages », Pakistan Journal of Botany 41(3), p 1197‑1204.
Murray PR, Niles AC. 1983. « Inoculum preparation for anaerobic susceptibility tests », Journal of Clinical Microbiology, 18(3), p 733‑734, sept. http://dx.doi.org/10.1128/jcm.18.3.733-734.1983.
Chawech R, Mhalla D, Trigui M, Mihoubi M, Fabre N, Jarraya R. 2015. « Chemical composition and antibacterial activity of extracts and compounds isolated from Citrullus colocynthis (L.) Schrad », Journal of Pharmacognosy and Phytochemistry 4(4), 197‑203.
Chaughule RS, Barve RS. 2023. « Role of herbal medicines in the treatment of infectious diseases », Vegetos, 37(1), 41‑51 p, janv. http://dx.doi.org/10.1007/s42535-022-00549-2.
Renata UC. 2022. Antibiotic Resistance in Bacteria—A Review.
Vaghasiya Y. 2009. « Screening of some medicinal plants for antimicrobial properties-phytochemical and pharmacological studies of a selected medicinal plant », PhD Thesis, Saurashtra University.
Omar Diao Badji, Nouhou Diaby, Bédié Mbow, Papa Biram Sy, Ibrahima Diallo, Birane Diop, Aïssatou Alioune Gaye, Alioune Fall, Mouhamadou Fofana, Seydou Niang, 2025. Phytochemical screening and determination of antimicrobial activity of different extracts from the roots and leaves of Stylocheaton Hypogaeus, a plant used for the treatment of prostatitis in Senegal. Int. J. Biosci., 26(2), 139-148.
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