Phytochemical screening, anti-diabetic and antioxidant potential of methanolic extract of Indigofera heterantha roots

Paper Details

Research Paper 01/05/2017
Views (993)
current_issue_feature_image
publication_file

Phytochemical screening, anti-diabetic and antioxidant potential of methanolic extract of Indigofera heterantha roots

Muhammad Aurang Zeb, Muhammad Sajid, Taj Ur Rahman, Khanzadi Fatima Khattak, Muhammad Tariq Khan
Int. J. Biosci. 10(5), 355-360, May 2017.
Copyright Statement: Copyright 2017; The Author(s).
License: CC BY-NC 4.0

Abstract

The aim of the current study was to detect the Phytochemical present in the methanolic extract of the plant using the biochemical tests and to evaluate the anti-diabetic and antioxidant potential of the methanolic extract of I. heterantha roots using Glucose uptake yeast in cells assay and 1, 1-diphenyl -2-picrylhydrazyl (DPPH) free radical scavenging assay. The methanolic extract showed good anti-diabetic activity with minimum increase 5.31% at 10µg/ml and maximum 53.19% at 80μg/ml glucose concentration while moderate antioxidant activity with minimum radical scavenging activity 22.00% at 200µg/ml and maximum activity 56.35% at 1000µg/ml. The results obtained revealed that this plant is very important from medicinal point of view.

Abubakar MS, Balogun E, Abdurahman  EM, Nok AJ, Shok M, Mohammed A, Garba M. 2006. Ethnomedical Treatment of Poisonous Snakebites: Plant Extract Neutralized Najanigricollis. Venom. Pharmaceutical biology 44(5), pp 343-348.

Amala BE, Ganga N, Arivudainambi R, Santhanam G. 1982. Anti in flammatory activity of Indigofera as palathoides Vahl. The Indian journal of medical research 76, p 115.

Blois MS. 1958. Antioxidant determinations by the use of a stable free radical.

Chakrabarti R, Damarla RK, Mullangi R, Sharma VM, Vikramadithyan RK, Rajagopalan R. 2006. Insulin sensitizing property of Indigofera my sorensis extract. Journal of ethno-pharmacology 105(1), pp 102-106.

Dahot  MU. 1999. Antibacterial and antifungal activity of small protein of Indigofera oblongifolia leaves. Journal of ethnopharmacology 64(3), pp. 277-282.

Esimone CO, Adikwu MU, Muko KN. 1999. Antimicrobial properties of Indigofera dendroides leaves. Fitoterapia  70(5), pp 517-520.

Gamble JS. 1984. A manual of Indian timbers. Bishen Singh Mahendra Pal Singh.

Ghias Uddin, Taj Ur Rehman, Mohammad Arfan, Wajiha Liaqat, Waliullah, Ghulam Mohammad, Abdur Rauf, Inamullah Khan, Mohammad Iqbal Choudhary. 2011. Journal of Medicinal Plants Research 5, 5735.

Hamayun  M, Khan A, Khan MA. 2003. Common medicinal folk recipes of District Buner, NWFP, Pakistan.  Ethno-botanical Leaflets  2003(1), p.14.

Hamayun M, Afzal S, Khan MA. 2006. Ethno-pharmacology, indigenous collection and preservation techniques of some frequently used medicinal plants of Utror and Gabral, district Swat, Pakistan. African Journal of Traditional, Complementary and Alternative Medicines 3(2), pp 57-73.

Herborne JB. 1973. Photochemical Methods 3Edn. D.E. and Hall Ltd. London  pp 135-203.

Maier A, Völker B, Boles E, Fuhrmann GF. 2002. Characterization of glucose transport in Saccharomyces cerevisiae with plasma membrane vesicles (Counterturn an sport) and intact cells (initial uptake) with single Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 or Gal2 transporters. FEMS yeast research 2(4), pp 539-550.

Malik A, Riaz N, Ahmad H, Nawaz SA, Choudhary MI. 2005. Lip oxygenase inhibiting constituents from Indigofera hetrantha. Chemical and Pharmaceutical Bulletin 53(3), pp 263-266.

Prakash D, Suri S, Upadhyay G, Singh BN. 2007. Total phenol, antioxidant and free radical scavenging activities of some medicinal plants. International Journal of Food Sciences and Nutrition 58(1), pp 18-28.

Shahjahan M, Vani G, Devi CS. 2005. Protective effect of Indigofera oblongifolia in CCl4-induced hepatotoxicity. Journal of medicinal food 8(2), pp 261-265.

Sharif A, Ahmed E, Malik A, Riaz N, Afza N, Nawaz SA, Arsha M, Shah MR, Choudhary  MI. 2005. Lip oxygenize inhibitory constituents from Indigofera oblongfolia. Archives of Pharmacia research 28(7), pp 761-764.

Shinwari  ZK, Watanab T, Rehman  M, Youshikawa T. 2006. A pictorial guide to Medicinal Plants of Pakistan. KUST. Kohat Pakistan.

Sittie AA, Nyarko AK. 1998. Indigofera arrecta: safety evaluation of an anti-diabetic plant extract in non‐diabetic human volunteers. Phototherapy Research 12(1), pp 52-54.

Sofora A. 1993. Medicinal plants and Traditional Medicine in Africa. John Wiley and Son Ltd. pp 150-153.

Trease GE, Evans WC. 1989. Pharmacology, 11th Edtn. Brailliar Tiridel and Macmillian Publishers London.

Waako PJ, Katuura E, Smith P, Folb P. 2007. East African medicinal plants as a source of lead compounds for the development of new antimalarial drugs. African Journal of Ecology 45(s1), pp.102-106.

Related Articles

Characterization of juvenile growth of néré (Parkia biglobosa (Jacq.) R.Br. ex G. Don) in Northern Côte d’Ivoire

Koffi Adjoua Bénédicte*, Tra Bi Tra Jeannot, Sanogo Yacouba, Keita Abdoul Karim, Silue Pagadjovongo Adama, Soro Dramane, Int. J. Biosci. 29(4), 60-69, October 2026.

Ethnobotanical survey and pharmacological evaluation of aphrodisiac plants used in the department of Oumé (Central-West Côte d’Ivoire)

Moyabi Any Georges Armel*, Kpan Wokapeu Blaise, Tra Bi Zamblé Fidèle, Coulibaly Founzégué Amadou, Koné Mamidou Witabouna, Int. J. Biosci. 29(4), 38-47, October 2026.

When silence turns to pain: Decoding endodontic flare-ups

S. Vidya, T. Vinay Kumar Reddy*, K. Vijay Venkatesh, B. Shyam, Seetha Kunhikannan, Int. J. Biosci. 29(4), 23-37, October 2026.

Effect of rubber tree seed cake on the vegetative growth of oil palm in a nursery in La Mé, Southeast of Côte d’Ivoire

Adou Bini Yao Christophe*, Yoboue Ahou Natachat, Kelechi Nwanchor, Coulibaly koigni Junior Pacome, Konan Djézou, Kouakou Tanoh Hilaire, Int. J. Biosci. 29(4), 14-22, October 2026.

Diversity, botanical origin, and valorization potential of honeys from Lower Guinea, Guinea: A systematic descriptive review

Aude Eminsédé Kelomey*, Jean Loua, Steven Chokki, Ibrahima Kalil Sylla, Doumbouya Almamy Daouda, Elhadj Saidou Balde, Int. J. Biosci. 29(4), 1-13, October 2026.

Effects of crop succession on bird communities in an urban wetland in Korhogo (Côte d’Ivoire)

Konan Ekoun Michaël*, Niamien Coffi Jean Magloire, Guétondé Valérie Florence, Yéo N’gan-Nan, Yaokokoré-Béibro Kouassi Hilaire, Int. J. Biosci. 29(3), 94-107, September 2026.