Phytochemical screening, anti-diabetic and antioxidant potential of methanolic extract of Indigofera heterantha roots
Paper Details
Phytochemical screening, anti-diabetic and antioxidant potential of methanolic extract of Indigofera heterantha roots
Abstract
The aim of the current study was to detect the Phytochemical present in the methanolic extract of the plant using the biochemical tests and to evaluate the anti-diabetic and antioxidant potential of the methanolic extract of I. heterantha roots using Glucose uptake yeast in cells assay and 1, 1-diphenyl -2-picrylhydrazyl (DPPH) free radical scavenging assay. The methanolic extract showed good anti-diabetic activity with minimum increase 5.31% at 10µg/ml and maximum 53.19% at 80μg/ml glucose concentration while moderate antioxidant activity with minimum radical scavenging activity 22.00% at 200µg/ml and maximum activity 56.35% at 1000µg/ml. The results obtained revealed that this plant is very important from medicinal point of view.
Abubakar MS, Balogun E, Abdurahman EM, Nok AJ, Shok M, Mohammed A, Garba M. 2006. Ethnomedical Treatment of Poisonous Snakebites: Plant Extract Neutralized Najanigricollis. Venom. Pharmaceutical biology 44(5), pp 343-348.
Amala BE, Ganga N, Arivudainambi R, Santhanam G. 1982. Anti in flammatory activity of Indigofera as palathoides Vahl. The Indian journal of medical research 76, p 115.
Blois MS. 1958. Antioxidant determinations by the use of a stable free radical.
Chakrabarti R, Damarla RK, Mullangi R, Sharma VM, Vikramadithyan RK, Rajagopalan R. 2006. Insulin sensitizing property of Indigofera my sorensis extract. Journal of ethno-pharmacology 105(1), pp 102-106.
Dahot MU. 1999. Antibacterial and antifungal activity of small protein of Indigofera oblongifolia leaves. Journal of ethnopharmacology 64(3), pp. 277-282.
Esimone CO, Adikwu MU, Muko KN. 1999. Antimicrobial properties of Indigofera dendroides leaves. Fitoterapia 70(5), pp 517-520.
Gamble JS. 1984. A manual of Indian timbers. Bishen Singh Mahendra Pal Singh.
Ghias Uddin, Taj Ur Rehman, Mohammad Arfan, Wajiha Liaqat, Waliullah, Ghulam Mohammad, Abdur Rauf, Inamullah Khan, Mohammad Iqbal Choudhary. 2011. Journal of Medicinal Plants Research 5, 5735.
Hamayun M, Khan A, Khan MA. 2003. Common medicinal folk recipes of District Buner, NWFP, Pakistan. Ethno-botanical Leaflets 2003(1), p.14.
Hamayun M, Afzal S, Khan MA. 2006. Ethno-pharmacology, indigenous collection and preservation techniques of some frequently used medicinal plants of Utror and Gabral, district Swat, Pakistan. African Journal of Traditional, Complementary and Alternative Medicines 3(2), pp 57-73.
Herborne JB. 1973. Photochemical Methods 3Edn. D.E. and Hall Ltd. London pp 135-203.
Maier A, Völker B, Boles E, Fuhrmann GF. 2002. Characterization of glucose transport in Saccharomyces cerevisiae with plasma membrane vesicles (Counterturn an sport) and intact cells (initial uptake) with single Hxt1, Hxt2, Hxt3, Hxt4, Hxt6, Hxt7 or Gal2 transporters. FEMS yeast research 2(4), pp 539-550.
Malik A, Riaz N, Ahmad H, Nawaz SA, Choudhary MI. 2005. Lip oxygenase inhibiting constituents from Indigofera hetrantha. Chemical and Pharmaceutical Bulletin 53(3), pp 263-266.
Prakash D, Suri S, Upadhyay G, Singh BN. 2007. Total phenol, antioxidant and free radical scavenging activities of some medicinal plants. International Journal of Food Sciences and Nutrition 58(1), pp 18-28.
Shahjahan M, Vani G, Devi CS. 2005. Protective effect of Indigofera oblongifolia in CCl4-induced hepatotoxicity. Journal of medicinal food 8(2), pp 261-265.
Sharif A, Ahmed E, Malik A, Riaz N, Afza N, Nawaz SA, Arsha M, Shah MR, Choudhary MI. 2005. Lip oxygenize inhibitory constituents from Indigofera oblongfolia. Archives of Pharmacia research 28(7), pp 761-764.
Shinwari ZK, Watanab T, Rehman M, Youshikawa T. 2006. A pictorial guide to Medicinal Plants of Pakistan. KUST. Kohat Pakistan.
Sittie AA, Nyarko AK. 1998. Indigofera arrecta: safety evaluation of an anti-diabetic plant extract in non‐diabetic human volunteers. Phototherapy Research 12(1), pp 52-54.
Sofora A. 1993. Medicinal plants and Traditional Medicine in Africa. John Wiley and Son Ltd. pp 150-153.
Trease GE, Evans WC. 1989. Pharmacology, 11th Edtn. Brailliar Tiridel and Macmillian Publishers London.
Waako PJ, Katuura E, Smith P, Folb P. 2007. East African medicinal plants as a source of lead compounds for the development of new antimalarial drugs. African Journal of Ecology 45(s1), pp.102-106.
Muhammad Aurang Zeb, Muhammad Sajid, Taj Ur Rahman, Khanzadi Fatima Khattak, Muhammad Tariq Khan, 2017. Phytochemical screening, anti-diabetic and antioxidant potential of methanolic extract of Indigofera heterantha roots. Int. J. Biosci., 10(5), 355-360.
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