Phytochemical screening, antioxidant, anti-biofilm and antifungal activities of aqueous and ethanolic extracts of Euphorbia hirta, Citrus aurantifolia and Heterotis rotundifolia in Benin

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Phytochemical screening, antioxidant, anti-biofilm and antifungal activities of aqueous and ethanolic extracts of Euphorbia hirta, Citrus aurantifolia and Heterotis rotundifolia in Benin

Oladélé Gautier Roko, Victorien Dougnon, Armelle Hounkpatin, Jean Robert Klotoe, Haziz Sina, Durand Dah-Nouvlessounon, Kamirou Chabi-Sika, Lamine Baba-Moussa
Int. J. Biosci.17( 6), 293-304, December 2020.
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Abstract

Euphorbia hirta, Citrus aurantifolia and Heterotis rotundifolia are plants of the traditional Beninese medicine. They are used in the treatment of several diseases, such as diarrheal infections, salmonellosis, malaria and others. This study aims to contribute to the scientific knowledge of these plants through their phytochemical screening as well as the in vitro evaluation of the antioxidant, antibiofilm and antifungal activity of their aqueous and ethanolic extracts. The phytochemical screening of these plants was evaluated by the standard method. The antioxidant activity was determined through the DPPH radical and the FRAP method. Anti-biofilm and antifungal activities were performed using the colorimetric method and the mycelial growth inhibition method respectively. Phytochemical screening of these plants revealed the presence of secondary metabolites such as: tannins, flavonoids, anthocyanins, quinonic derivatives etc. The aqueous and ethanolic extracts of these plants have antioxidant properties with IC50s ranging from 23.7 to 55.36 µg/ml (DPPH) and 0.43 to 1.96 mg/ml (FRAP). The anti-biofilm and antifungal effects of these extracts vary according to strains, extracts and plants. The inhibition percentages of bacterial biofilm are between 20.66 and 81%. The highest percentage of mycelial growth inhibition is 69.23±1.86% obtained with the ethanolic extract of Euphorbia hirta on Fusarium solani. The antioxidant, anti-biofilm and antifungal activities are much more remarkable with ethanolic extracts. These different results may justify the use of these plants in traditional medicine. Euphorbia hirta, Citrus aurantifolia and Heterotis rotundifolia are therefore of medicinal interest as a potential source of natural bioactive substances.

VIEWS 71

Adinortey MB. 2015. Pharmacological and toxicological evaluation in rats of Dissotis rotundifolia, a medicinal plant traditionally used in Ghana for the management of peptic ulcer. PhD Thesis. Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences. Kwame Nkrumah University of Science & Technology, Kumasi, Ghana, p 229.

Akoua KC, Guessend N, Gbonon V, Faye-kette AYH, Dosso M. 2004. Methicillini-resistant of S. aureus activity in Abidjan (1998-2001): A new hospital problem. Medecines Maladies Infectieuses. 34(3), 132-36.

Al Namani J, Baqir E, Al Abri A, Al Hubaishi T, Husain A, Khan SA. 2018: Phytochemical Screening, Phenolic Content and Antioxidant Activity of Citrus aurantifolia L. Leaves Grown in Two Regions of Oman. Iranian Journal of Pharmaceutical Sciences 14(1), 27-34.

Amic D, Beslo D, Trinajstic N. 2003. Structure-radical scavenging activity relasionships of flavonoids. Croatica Chemical Acta. 76, 55-61.

Ayéna AC, Agassounon M, Adoukonou-Sagbadja H, Mensah GA, Agbangla C, Baba-moussa L, Ahanhanzo C. 2017 : Potentiels antimicrobiens de Euphorbia hirta L. et de Phyllanthus amarus schumach & thonn, deux Euphorbiaceae utilisées dans le traitement des gastroentérites au Sud du Bénin. Revue de microbiologie industrielle, sanitaire et environnementale 11(1), 1-15.

Balogun OS, Owoseni AA. 2013. Phytochemical and antimi-crobial investigation of Dissotis rotundifolia (sm) Triana. Journal of Natural Products 9(2), 77–80.

Bourgaud F, Gravot A, Milesi S, Gontier E. 2001. Production of plant secondary metabolites: a historical perspective. Plant Science 161, 839-851.

Chitra M, Muga V, Sasikumar D, Awdah M. 2011: Screening of Phytochemical and In vitro activity of Euphorbia hirta L. Journal of Chemical and Pharmaceutical Research 3(6), 110-114.

Codoñer-Franch P, Valls-Belles V, Arilla- Codoñer A, Alonso-Iglesias E. 2011 : Oxidant mechanisms in childhood obesity: the link between inflammation and oxidative stress. Translational Research 158(6), 369-384.

Dougnon VT, Klotoé JR, Sènou M, Roko GO, Dougnon G, Fabiyi K, Amadou A, Aniambossou A, Assogba P, Bankolé H, Dougnon J, Baba-Moussa L. 2017: Chemical Composition, Cytotoxicity and Antibacterial Activity of Selected Extracts of Euphorbia hirta, Citrus aurantifolia and Heterotis rotundifolia on Enteropathogenic Bacteria. EC Microbiology 12(4), 180-195.

Ezikanyi DN, Nnamani CV, Ihejirika JN. 2016. Qualitative Phytochemical Analysis and in Vitro Fungal Activities of Citrus aurantifolia L. and Carica papaya L. Leaves Extracts on Pathogenic Fungi. Journal of Pharmacy and Biological Sciences 11(3), 68-72.

Fasola TR, Iyamah PC. 2014. Comparing the Phytochemical Composition of Some Plant Parts Commonly Used in the Treatment of Malaria. International Journal of Pure and Applied Sciences and Technology 21(1), 1-11.

Guede-Guina F, Vangah-Mandah M, Bonga G, M, de Souza C. 1995. Activité antibactérienne d’un extrait végétale contre les germes opportunistes au cours du SIDA. Revue de Médecine et de Pharmacie. 9, 13-19.

Guillemot D, Maugendre P, Vhauvin Sermé TC. 2004. Consommation des antibiotiques en France. Bulletin épidémiologique hebdomadaire (BEH) 3233, 141-147.

Harborne JB. 1998. Phytochemical methods: A guide to modern techniques of plant analysis. 3rd ed. Chapman and Hall Int. (Ed).NY, 49–188.

Houghton PJ, Raman A. 1998. Laboratory Handbook for the Fractionation of Natural Extracts. 1 ère édition. Chapman and Hall, p 244.

Kar A. 2007. Pharmacognosy and Pharmabiotechnologie. 2nd Ed: New Age International Publishers. 1-30.

Karanga Y, Ilboudo O, Bonzi S, Tapsoba I, Somda I, Bonzi-Coulibaly YL. 2017. Phytochemical and Antifungal Properties of Euphorbia hirta L against Fusarium moliniforme and Phoma sorghina. Natural Products: An Indian Journal 13(1), 105-114.

Kelly EH, Tagliaferro AR, Bobilya DJ. 2002. Flavonoid antioxidant: chemistry, metabolism and structure-activity relationships. Journal of Nutritional Biochemistry 13, 572-584.

Lagnika L, Anagonou E, Atindehou M, Adjahoutonon B, Dramane K, Sanni A. 2011. Antimicrobial activity of Crateva religiosa Forst against bacteria isolated from thryonomys swinderianus temminckAfrican Journal of. Biotechnology 10, 9-10034.

Mansouri A, Embarek G, Kokkalou E, Kefalas P. 2005. Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chemistry 89, 411-420.

Millogo-Koné H, Asimi S, Guissou IP, Nacoulma OG. 2008. Etude de l’activité antimicrobienne d’extraits de Parkia biglobosa (JACQ.) BENTH. sur des souches de Staphylococcus aureus. Pharmacopée et Médecine traditionnelle Africaines 15, 1-5.

N’Guessan JD, Bidie AP, Lenta BN, Weniger B, Andre P, Guede-Guina F. 2007. In vitro assays for bioactivity-guided isolation of anti-Salmonella and antioxidant compounds in Thonningia sanginea flowers. African Journal of Biotechnology 6, 1685-1689.

Nacoulma O. 1996. Plantes médicinales et pratiques médicales traditionnelles au Burkina Faso, cas du plateau central. Thèse Doctorat d’Etat es Sciences Naturelles. Université de Ouagadougou, p 210.

Ndam P, Onyemelukwe N, Nwakile CD, Ogboi SJ, Maduakor U. 2018. Antifungal properties of methanolic extracts of some medical plants in Enugu, South East Nigeria. African Journal of Clinical and Experimental Microbiology 19(2), 141-148.

Nweke FU. 2015. Effect of Citrus Aurantifolia Leaf Extract on Mycelial Growth and Spore Germination of Different Plant Pathogenic Fungi. Advances in Life Science and Technology 31, 2224-7181.

Sanchez-Moreno C. 2002. Methods used to evaluate the free radical scavenging activity in foods and biological systems. International Journal of Food Science & Technology 8(3), 121-137.

Sanogo R, Diallo D, Diarra S, Ekoumou C, Bougoudogo F. 2006. Activité antibactérienne et antalgique de deux recettes traditionnelles utilisées dans le traitement des infections urinaires et la cystite au Mali. Mali Médical 21, 18-24.

Sarr SO, Fall AD, Gueye R, Diop A, Diatta K, Diop N, NDiaye B, Diop YM. 2015. Etude de l’activité antioxydante des extraits des feuilles de Vitex doniana (Verbenaceae). International Journal of Biological and Chemical Sciences 9(3), 1263-1269.

Suhaj M. 2006. Spice antioxidants isolation and their antiradical activity: a review. Journal of Food Composition and Analysis 19, 531–537.

Svoboda K, Svoboda T. 2000. Secretory structures of aromatic and medicinal plants. Ed: Microscopix Publications 7-12.

Tadhani MB, Patel VH, Subhash R. 2007. In vitro antioxidant activities of Stevia rebaudiana leaves and callus. Journal of Food Composition and Analysis 20, 323-329.

Tyler VE. 1993. The honest herbal. 3rd ed. Pharmaceutical Products Press (Haworth Press) Binghamton, NY. p 375.

Nikolić M, Vasić S, Đurđević J, Stefanović O, Čomić L. 2014. Antibacterial and anti-biofilm activity of ginger (Zingiber officinale (Roscoe)) ethanolic extract. Kragujevac Journal of Science 36, 129-136.

Tutar U, Çelik C, Karaman I, Ataş M, Hepokur C. 2016. Anti-biofilm and antimicrobial activity of Mentha pulegium L essential oil against multidrug-resistant Acinetobacter baumannii. Tropical Journal of Pharmaceutical Research 15(5), 1039-1046.

Dieng SIM, Fall AD, Diatta-Badji K, Sarr A, Sene M, Sene M, Mbaye A, Diatta W, Bassene E. 2017. Evaluation de l’activité antioxydante des extraits hydro-ethanoliques des feuilles et écorces de Piliostigma thonningii Schumach. International Journal of Biological and Chemical Sciences 11(2), 768-776.

Vikram A, Jayaprakasha GK, Jesudhasan PR, Pillai SD, Patil BS. 2010. Suppression of bacterial cell-cell signalling, biofilm formation and type III secretion system by citrus flavonoids. Journal of Applied Microbiology 109(2), 515–527.

World Health Organization (WHO). 2002. Stratégies de l’Organisation Mondiale de la Santé pour la médecine traditionnelle 2002-2005.WHO /EDM /TRM /2002.1.