Phytochemical study of three endemic brooms in Algeria: Genista numidica Spach, Genista ferox Poiret and Genista tricuspidata Desf
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Phytochemical study of three endemic brooms in Algeria: Genista numidica Spach, Genista ferox Poiret and Genista tricuspidata Desf
Abstract
Genista numidica, Genista ferox and Genista tricuspidata are three endemic plants belonging to the family Fabaceae and which remain very little studied. The purpose of this work is to evaluate the antioxidant power of methanolic extracts of the flowers and the leaves of these species according to the DPPH free radical scavenging method while determining their total phenol, total flavonoids and tannin contents (the secondary metabolite), Flavonoids were estimated by the aluminum chloride (AlCl3) method, the total phenols were determined with Folin-Ciocalteu reagent and the tannins were estimated by the vanillin in acid medium method. The investigations showed that G. numidica is the most rich in polyphenols (113 ± 3.87mg and 79.6 ± 3.49 mg) of gallic acid equivalent and it’s the most flavonoid-rich with 85.47 ± 1.51mg/g and 87.31mg/g of quercetin equivalent in flowers and leaves respectively. However, G. ferox was the most-rich in tannins in both leaves and flowers with 39.81 ± 0.32 mg/g and 44.36 mg /g of catechin equivalent respectively. G. tricuspidata showed a high level in the flowers extract (39.36 ± 0.32mg / g) and G.numidica gave 37.77 ± 0.64mg / g in the leaves extract. The antioxidant activity was the best in leaves and flowers extracts of G. ferox (IC50 = 0.50 ± 0.006 mg/ml and 0.59 ± 0.02mg /ml) as well as G.numidica leaf extract (0.55 ± 0.03 mg /ml). These results showed that this species would be suggested as a promising alternative source of the natural anti-oxidative phenolic compounds.
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Samira Ati, Bennadja Salima, Boumaraf Warda, 2017. Phytochemical study of three endemic brooms in Algeria: Genista numidica Spach, Genista ferox Poiret and Genista tricuspidata Desf. Int. J. Biosci., 11(3), 178-183.
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