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Phytochemicals, antioxidants activity, total phenolic and total flavonoid content of liquid smoke from tropical plants

Research Paper | December 7, 2022

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Erina Hertianti, Wiwin Suwinarti, Bandi Supraptono, Farida Aryani, Irawan Wijaya Kusuma

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Int. J. Biosci.21( 6), 316-321, December 2022

DOI: http://dx.doi.org/10.12692/ijb/21.6.316-321


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Liquid smoke is condensation from the carbonization process and contains component of cellulose, hemicellulose and lignin. The problem is liquid smoke were only a side product on charcoal making process and the used of raw materials also liquid smoke productions are very limited. This research focused on the liquid smoke applications of 15 tropical plants. Research objectives in this study are utilized the 15 tropical plants leaves as raw material for liquid smoke production; analyzing the liquid smoke quality based on phytochemicals, antioxidant activity, total phenolic and total flavonoid content; also calculating the yield of liquid smoke. The phytochemical screening was evaluated by Harborne and Kokate methods. Total phenolic content was determined by Folin-Ciocalteu method, while total flavonoid content was determined by Colometric assay method. The results showed the liquid smoke from tropical plant leaves contains alkaloids, flavonoids and tannins. The IC50 value range from 0.026 ppm-0.431 ppm. The liquid smoke samples tested contained 0.0096 to 0.0415mg of Gallic Acid Equivalents (GAE)/g extract represented the total phenolic content. The flavonoid content of the liquid smoke samples were in range from 0.0006 to 0.1759mg of Quercetin Equivalents (QE)/g extract. The yield of liquid smoke range between 2.81%-49.66%.


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Phytochemicals, antioxidants activity, total phenolic and total flavonoid content of liquid smoke from tropical plants

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