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Phytosynthesis of silver nanoparticles using Tamrixa phylla and biological applications

Asif Jan, Humerah Jan, Gulbakhta Jan, Ijaz Naeem, Mubarak Ali Khan

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Int. J. Biosci.15(2), 600-611, August 2019

DOI: http://dx.doi.org/10.12692/ijb/15.2.600-611


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Due to biocompatibility, antimicrobial activities and other medicinal properties, silver nanoparticles (Ag-NPs) have drawn significant attention for bio-medical applications. The present study focused on eco-friendly synthesis of Ag-NPs using Tamarix aphylla aqueous extract. The synthesized Ag-NPs were characterized by UV-Vis spectrophotometer and their characteristic Surface Plasmon Resonance (SPR) peak was observed at (435nm). Fourier Transform Infrared (FT-IR) spectrophotometer was used to confirm formation of the Ag-NPs and to find out the specific functional groups responsible for reduction of AgNO3. The morphology of Ag-NPs was characterized by Scanning Electron Microscopy (SEM) and find out that nanoparticles were spherical and in the size range of 4-48nm. Energy Dispersive X-ray (EDX) was used for elemental analysis to detect the presence of elemental silver. X-Ray Diffraction (XRD) was used to determine the crystalline nature, purity and average particle size of nanoparticles. The average particle size was observed to be approximately 22nm. Moreover, the antibacterial potential of synthesized Ag-NPs was tested against six selected pathogenic bacteria, and five fungal strains. The nanoparticles showed strong anti-bacterial and antifungal activities. Nonetheless, in culture cells Ag-NPs bound to outer proteins of viral particles and inhibited the binding site and also replication.


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Phytosynthesis of silver nanoparticles using Tamrixa phylla and biological applications

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