Plant and microbes interaction: the potential of Calotropis gigantea extract and latex towards bacterial strains
Paper Details
Plant and microbes interaction: the potential of Calotropis gigantea extract and latex towards bacterial strains
Abstract
The microbial resistance has prominently diminished the efficacy of antibiotics and also the major cause of failure to treat bacterial infections. In the backdrop of this phenomenon, phytochemical or herbal medicines are gaining attention as the alternative source of natural antibiotics. In the present study, leaves extract and milk (latex) of Calotropis gigantea were tested for antibacterial activity. For this purpose, well diffusion method was evaluated fortwo-Gram positive (Staphylococcus aureus, Streptococcus pyogenes) and two-Gram negative (Escherichia coli, Klebsiella pneumonia) bacterial strains. Results revealed significant antimicrobial activities of the tested samples. The inhibition zones ofbacterial strains were observed in the range of 9 to 32 mm. At a concentration of 5 mg/ml leaves,activity was found good against S. pyogenes and K. pneumoniae while 16 days old milk (latex) against S. aureus. Excellent zones of inhibition were showed by latex against E. coli at a concentration of 10 mg/ml. E. coli and K. pneumoniae showed resistant to leaves extract and fresh milk (latex) at 15 mg/ml concentration. Leaves extract at a concentration of 20 mg/ml showed excellent inhibitory zones against S. aureus. Moreover, extract showed excellent inhibitory zones to S. pyogenes as compare to 5 days old milk (latex) at a concentration of 50 mg/ml. Furthermore, it was concluded that leaves extract and milk (latex) of C. gigantea could be used as an alternative to antibiotics against different diseases because of their substantial antibacterial activities.
Cowan ST. 2003. Cowan and Steel’s manual for the identification of medical bacteria. Cambridge university press.
Davies J. 1994. Inactivation of antibiotics and the dissemination of resistance genes. Science 264(5157), 375-382. http://dx.doi.org/10.1126/science.8153624
Essawi T, Srour M. 2000. Screening of some Palestinian medicinal plants for antibacterial activity. Journal of ethnopharmacology 70(3), 343-349. http://dx.doi.org/10.1016/S0378-8741(99)00187-7
Jayakumar D, Mary SJ, Santhi RJ. 2010. Evaluation of antioxidant potential and antibacterial activity of Calotropis gigantea and Vinca rosea using in vitro model. Indian Journal of Science and Technology 3(7), 720-723.
Kumar G, Karthik L. Rao KVB. 2010. Antibacterial activity of aqueous extract of Calotropis gigantea leaves–an in vitro study. International journal of pharmaceutical Sciences Review and Research 4(2), 141-144.
Mahmood A, Mahmood A, Shaheen H, Qureshi RA, Sangi Y, Gilani SA. 2011. Ethno medicinal survey of plants from district Bhimber Azad Jammu and Kashmir, Pakistan. Journal of Medicinal Plants Research 5(11), 2348-2360.
Nenaah EG, Ahmed ME. 2011. Antimicrobial activity of extracts and latex of Calotropis procera (Ait.) and synergistic effect with reference antimicrobials. Research Journal of Medicinal Plant, 5(6), 706-716. http://dx.doi.org/10.3923/rjmp.2011.706.716
Newman DJ, Cragg GM, Snader KM. 2000. The influence of natural products upon drug discovery. Natural product reports 17(3), 215-234. http://dx.doi.org/10.1039/A902202C
Nobel PS. 1980. Morphology, nurse plants, and minimum apical temperatures for young Carnegiea gigantea. Botanical Gazette 141(2), 188-191.
Rahman M, Aziz A, Moon SS. 2007. Antioxidant polyphenol glycosides from the plant Draba nemorosa. Bulletin of the Korean Chemical Society 28(5), 827-831. http://dx.doi.org/10.5012/bkcs.2007.28.5.827
Saket RB, Singh S. 2017. Study of certain medicinal plants of Sidhi district and their antimicrobial activity against plant and human pathogens. International journal of advanced research 3(3), 675-679.
Subramanian SP, Saratha V. 2010. Evaluation of antibacterial activity of Calotropis gigantea latex extract on selected pathogenic bacteria. Journalof Pharmacy Research 3, 517-21. http://dx.doi.org/10.4103/0972-0707.168809
Wang ZN, Wang MY, Mei WL, Han Z, Dai HF. 2008. A new cytotoxic pregnanone from Calotropis gigantea. Molecules 13(12), 3033-3039. https://doi.org/10.3390/molecules13123033
Wilson D. 1995. Endophyte: the evolution of a term, and clarification of its use and definition. Oikos, 274-276. http://dx.doi.org/10.2307/3545919
Muhammad Numan, Iqbal Hussain, Ubaid Ahmad, Rahim Dost Khan, Mehmand Khan, Abdul Rehman, Warda Komal, Wali Muhammad, Syed Abdul Hadi, 2018. Plant and microbes interaction: the potential of Calotropis gigantea extract and latex towards bacterial strains. Int. J. Biosci., 13(6), 95-101.
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