Potato germplasm innovation through micro tuberization technique
Paper Details
Potato germplasm innovation through micro tuberization technique
Abstract
In vitro micro tuber formation potentiality of potato was investigated to establish a rapid disease free seed production system as well as potato germplasm innovation. Micro tubers were induced to meristem derived in vitro grown shoots in culture bottles increasing the concentration of BA (6-Benzyl adenine), KIN (Kinetin) and sucrose in MS (Murashige and Skoog 1962) medium. KIN showed better performance than BA for micro tuber induction and among the three concentrations (6-10 mg/l) of KIN, 8 mg/l KIN was the most effective and more preferred concentration for micro tuber induction. It was observed that increase the level of KIN also increased the percentage of tuberization, number and weight of micro tuber/shoot. By increasing the concentration of sucrose increased the presentence (%) of in vitro tuberization. The media containing 60 g/l was found to be the best among three concentrations (40-70 g/l) of sucrose tested followed by 70 g/l sucrose for micro tuber induction for all four potato cultivars.
Alam MF, Ahsan N, Hossain SA, Anisuzzaman M, Parvez S, Swaraz A M and Khan MR. 2003a. Effect of different sources and concentrations of carbon on in vitro tuberization in potato (Solanum tuberosum L.) cv. Diamant Bangladesh, J. Genet Biotech 4(122), 42-48.
Alam MF, Ahsan N, Hossain MM, Parvez S, Swaraz AM, Khan MR. 2003b Acceleration of microtuber induction in potato by cytokinin and photoperiod adjustment Chinese Potato J. 17 (5), 257-261.
Barker WG. 1953. A method for the in vitro culture of potato tubers. Science 118, 384-385.
Catechpole AH, Hillman J. 1969. Effect of ethylene on tuber initiation in (Solanum tuberosum L) Nature 223, 1387-1388.
Chapman HW. 1958. Tuberization in the potato plant Physiol. Plant 11, 215-244.
Cutter EG. 1978. Structure and development of the potato plant In: The potato crop: The scientific basis for improvement P.M. Harris (ed.), Chapman and Hall, London, p. 71-152.
Estrada RP, Tovar, Dodd’s JH, 1986, Induction of’ in vitro tubers in a board range of potato genotypes, Plant Cell, Tissue and Organ culture 7, 3-10
Forsline PL, Langille AR. 1976a. Endogenous cytokinins in Solanum tuberosum as influenced by photoperiod and temperature Physiol. Plant 34. 75-77.
Forsline PL, Langille AR. 1976b. An assessment of modifiying effect of kinetin on ill vitro tuberization of induced and non-induced tissues of Solanum tuberosum can J Bot. 54, 2513-2516.
Harmey MA, Crowley MP. Clinch PEM. 1966. the effect of growth regulators on tuberization of cultured stem pieces of Solanum tuberosum Eur. Potato J. 9. 146-157.
Hoque MI, Mila NB, Khan MS, Sarker RH. 1996. Shoot regeneration and in vitro microtuber formation in potato (Solanum tuberosum L) Bangladesh J Bot. 25. 87-93.
Hussey G, Stacey NJ. 1984. Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.) Ann Bot. 53, 565-578.
Hussey G, Stacey NJ. 1984. Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L) Annals of Botany 53. 565-578.
Ishak ZS, Imanishi Egashira H, Suzuki H. 1992. Effect of sterilization and medium composition on germination of tomato seeds (Lycopersicon esculentum Mill.) J Yamagata Agri for Soc 49, 7-11.
Katsura N, Okazawa Y, Tagawa T. 1970. Physiological studies on the potaato tissue culture in vitro 1 Effect of 2 natphtalene acetic acid and 2, 4-dichlorophynoxy acetic acid on the callus and root formation. Mem. Fac. Agric. Hokkaido Univ 7, 301-306.
Kefi SDA, Pavlista PER, Kachman D. 2001. Comparison of thidiazuron and two nitroguanidiens to kinetin on potato microtuberization in vitro under short and long days J Plant Growth Regulation 10 1007.
Koda Y, Okazawa Y. 1983. Influences of environmental, hormonal and nutritional factors on potato tuberization in vitro Japan Jour. Crop Sci. 52, (4) 582-591.
Lawrence RH, Barker WG. 1963. A study of tuberization in the potato (Solanum tuberosum L.) Amer. Potato J. 40, 349-356.
Lo FM, Irvine BR, Barker WB. 1972. In vitro tuberization of the common potato (Solanum tuberosum) is not a response to the osmotic concentration of the medium. Canadian J Bot. 50, 603-605.
Mes MG, Menge I. 1954. Potato shoot and tuber cultures in vitro Physiol Plant 7, 637-649.
Palmer CE, Smith OE. 1970. Effect of kinetin on tuber formation on isolated stolons of Solanum tuberosum L. culture in vitro; Physiol Plant 11, 303-314.
Palmer CE, Smith OE. 1970. Effect of kinetin on tuber formation on isolated stolon of (Solaurnm tuberosum L) cultre in vitro Plant Cell Physiol 11 ,303-314.
Palmer CE, Smith OE. 1969. Cytokinins and tuber initiation in the potato (Solanum tuberosum L) Nature 221, 279-280.
Plaisted PH. 1957. Growth of the potato tuber Physiol Plant 32, 45-453.
Poehlamn MJ, Bortihakur D. 1977. Breeding potato In: Breeding Asian field crop, Oxford & IBH Publishing Co. p. 258-271.
Slater JW. 1963. Mechanisms of tuber initiation In: The growth of potato London U.K p. 114-120.
Wang PJ, Hu C. 1982. in vitro mass tuberization and virus-free seed potato production in Taiwan Amer Pot J. 59, 33-37.
Wattimena GA. 1983. Micropropagation as an alternative technology of potato production in Indonesia PhD Thesis University of Wisconsin, Madison, Wisconsin USA.
Ziv MZ, Shemesh D. 1996. Propagation and tuberization of potato bud cluster from bioreactor culture. In vitro Cell Development Biol Plant 32, 31-36.
Hoque ME. 2010. In vitro tuberization in potato (Solanum tuberosum L) POJ 3(1), 7-11.
Al-Momani F, Shibli R, Ajloum M. 2000. In vitro performance of potato (Solanum tuberosum L.) cv Spunta. J Agrotrop 11, 31-4.
Amma K, Maity S. 1998. Role of nodal position and hormones on micro tuber production in potato (Solanum tuberosum L) J Hort. 11, 65-7.
Coleman KW, Danielle JD, Colleman SE. 2001. Potato microtuber as research tools: A Review. Am J Potato Res. 78, 47-55.
El-Sawy A, Bekheet S, Aly UI. 2007. Morphological and molecular characterization of potato microtubers production on coumarin iducing medium. In J Agri Biol. 9(5), 675-680.
M. Nasiruddin, Maliha, Humayra Armin, M. Rezaul Karim, M. Monzur Hossain (2013), Potato germplasm innovation through micro tuberization technique; IJB, V3, N1, January, P135-141
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