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Potato germplasm innovation through micro tuberization technique

M. Nasiruddin, Maliha, Humayra Armin, M. Rezaul Karim, M. Monzur Hossain

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Int. J. Biosci.3(1), 135-141, January 2013

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Abstract

In vitro micro tuber formation potentiality of potato was investigated to establish a rapid disease free seed production system as well as potato germplasm innovation. Micro tubers were induced to meristem derived in vitro grown shoots in culture bottles increasing the concentration of BA (6-Benzyl adenine), KIN (Kinetin) and sucrose in MS (Murashige and Skoog 1962) medium. KIN showed better performance than BA for micro tuber induction and among the three concentrations (6-10 mg/l) of KIN, 8 mg/l KIN was the most effective and more preferred concentration for micro tuber induction. It was observed that increase the level of KIN also increased the percentage of tuberization, number and weight of micro tuber/shoot. By increasing the concentration of sucrose increased the presentence (%) of in vitro tuberization. The media containing 60 g/l was found to be the best among three concentrations (40-70 g/l) of sucrose tested followed by 70 g/l sucrose for micro tuber induction for all four potato cultivars.

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Potato germplasm innovation through micro tuberization technique

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