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Potential inoculant strains of Brazilian endophytic bacteria for maize (Zea mays L.) growth promotion

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Research Paper 01/10/2015
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Potential inoculant strains of Brazilian endophytic bacteria for maize (Zea mays L.) growth promotion

Vivian J. Szilagyi-Zecchin, Ana C. Klosowski, Angela C. Ikeda, Mariangela Hungria, Lygia V. Galli-Terasawa, Vanessa Kava-Cordeiro, Chirlei Glienke, Átila F. Mógor
Int. J. Agron. Agri. Res.7( 4), 128-134, October 2015.
Certificate: IJAAR 2015 [Generate Certificate]
Key: AuxinsBacillusPlant growth promotionPseudomonasSiderophores

Abstract

The maize (Zea mays L.) is one of the most important cereals as a source of energy for human nutrition and animal feed. And the cultivation of maize to be improved with the use of lower-impact resources for the environment and more profitable for farmers. Therefore the use of endophytic bacteria promoting plant growth comes against these ideals. Seven bacteria isolated from maize roots were identified and verified for their ability to promote plant growth under greenhouse conditions. In addition, mechanisms of plant growth promotion were investigated in vitro. Sequencing analysis of the 16S rRNA gene revealed five bacterial genera: Achromobacter, Enterobacter, Pseudomonas, Stenotrophomonas and Bacillus. Among the isolates, Bacillus sp. (LGMB227) promoted root length (65.1%), stimulated the aerial part development (39.4%) and Pseudomonas sp. (LGMB205) increased the root volume (22.7%). Here we demonstrate that in vivo data were corroborated by in vitro data, where the largest producer of IAA (63.1 mg/mL) was detected in Bacillus sp. LGMB227 that showed as well siderophore and pectinase activity. Pseudomonas sp. had the second largest production of IAA (56.1 mg/mL) and was also siderophore positive. Identified that strain LGMB227 of Bacillus sp. showed the best performance, with potential use as inoculants for plant growth promotion.

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