Preliminary screening of phytochemical contents, antioxidant and antibacterial activity of Alstonia scholaris (L.) R.Br collected in the Darrang area of Assam, India

Paper Details

Research Paper 01/03/2022
Views (406) Download (35)

Preliminary screening of phytochemical contents, antioxidant and antibacterial activity of Alstonia scholaris (L.) R.Br collected in the Darrang area of Assam, India

Lakhya Jyoti Gogoi, Sristisri Upadhyaya, Ranjan Sarma, Sahabuddin Ahmed
Int. J. Biosci.20( 3), 260-265, March 2022.
Certificate: IJB 2022 [Generate Certificate]


The current study was carried out to investigate the phytochemical contents, antioxidant activity, total phenolic content, total flavonoid content, and antibacterial activity of Alstonia scholaris (L.) R. Br. A preliminary phytochemical study was performed using conventional procedures. In crude aqueous, methanol, chloroform, and hexane extracts, total phenolic content, total flavonoid content, and antioxidant activity were measured spectrophotometrically. A well diffusion approach was used to assess in vitro antibacterial activity against seven Microbial Type Culture Collection (MTCC) bacterial species and two MTCC fungus species. Saponin, tannin, flavonoid, phenol, terpenoids, cardiac glycoside, steroid, and alkaloid were found in the preliminary phytochemical study, but not anthraquinone and reducing sugar. The methanol extract fared better than the other extracts. The methanolic extract exhibited clear antibacterial action against Staphylococcus epidermidis, Proteus vulgaris and Penicillium chrysogenum, whereas hexane extract showed modest activity against Penicillium chrysogenum. Using petroleum ether hot extract, petroleum ether showed better antimicrobial activity against Bacillus subtilis, Staphylococcus aureus and Penicillium chrysogenum, respectively. The greatest percentages of inhibition for DPPH and ABTS were 82.00±0.000 and 80.50±0.000 percent for methanol extracts, respectively. According to the current study, Alstonia scholaris (L.) R. Br includes both primary and secondary metabolites, which contribute to its traditional usage as a food and medicinal.


Akinyemi KO, Coker AO, Bayagbon C, Oyefolu AOB, Akinside KA, Omonigbehin EO. 2000. Antibacterial screening of five Nigerian medicinal plants against S. typhi and S. paratyphi, Journal of the Nigerian Infection Control Association 3(1), 1-7.

Chung KT, Wong TY, Wei CI, Huang YW, Lin Y. 1998. Tannins and human health: a review, Critical Review in Food Science and Nutrition 38(6), 421-464.

Herouart D, Sangwan RS, Fliniaux MA, Sangwan-Norreel BS. 1988. Variations in the leaf alkaloid content of androgenic diploid plants of Daturainnoxia, Planta Medica 54, 14-17. PMID: 17265195.

Islamy RA. 2019. Antibacterial activity of Cattle fish sp. (Cephalopoda) ink extract against Aeromonas hydrophila, Majalah Obat Tradisional 24(3), 184-188.

Jovale Vincent Tongco V, Remil Aguda M, Ramon Razal A. 2014. Proximate analysis, phytochemical screening and total phenolic and flavonoid content of Philippine bamboo Schizostachyumlumampao, Journal of Chemical and Pharmaceutical Research 6(1), 709-713.

Khyade MS Vaikos NP. 2009. Phytochemical and antibacterial properties of leaves of Alstoniascholaris R. Br.African Journal of Biotechnology 8(22), 6434-6436.

Karthishwaran K, Mirunalini S. 2012. Assessment of the antioxidant potential of Pergulariadaemia (Forsk.) Extract in vitro and in vivo experiments on hamster buccal pouch carcinogenesis, Asian Pacific Journal of Tropical Disease 31(2), 509-516.

Li Q, Zhao YL, Long CB, Zhu PF, Liu YP, Luo XD. 2019. Seven new veratramine type alkaloids with potent analgesic effect from Veratrumtaliense, Journal of Ethanopharmacology 244, 112137.

Molly Antony, Darsan Menon B, James Joel, Dev Lipin MS,  Arun K, Thankamani V. 2011. Phytochemical analysis and antioxidant activity of Alstoniascholaris, Pharmacognosy Journal 3(26), 13-18.

Mohammad Forhad Khan, Faisal Bin Kader, Mohammad Arman, Suhel Ahmed, Chadni Lyzu, Shahenur Alam Sakib, Shaifullah Mansur Tanzil, AFM Irfan Uddin Zim, Md. Abdus Shukur Imran, Tommaso Venneri, Barbara Romano Md. Areeful Haque, Raffaele Capasso. 2020. Pharmacological insights and prediction of lead bioactive isolates of Dita bark through experimental and computer-aided mechanism, Biomedicine & Pharmacotherapy 131, 110774.

Marjorie MC. 1999. Plant products as anti-microbial agents, Clinical Microbiology Reviews 12(4), 564-582.

Mahato SB, Sen S. 1997.  Advances in tri terpenoid research 1990-1994, Phytochemistry 44, 1185-1236.

Nair R, Chandra S. 2008. Antimicrobial activity of Terminaliacatappa, Manilkarazapota and Piperbetel Leaf Extract, Indian Journal Pharmaceutical Sciences 70(3), 390–393.

Nostro A, Germanò MP, D’angelo V, Marino A, Cannatelli MA. 2000. Extraction methods and bio autography for evaluation of medicinal plant antimicrobial activity, LettApplMicrobiol 30, 379-384.

Nadkarni AK. 1976. Indian MateriaMedica, 3rd ed. Mumbai, India: Popular Press.

Singh A, Dayal R, Ojha RP, Mishra KP. 2016,Phytocomponents of Argyreiaspeciosa (Linn. f) Confer radioprotection, Journal of Radiant and Cancer Research 7, 99-102.

Shang JH, Cai XH, Zhao YL, Feng T, Luo XD. 2010. Pharmacological evaluation of Alstoniascholaris: anti-tussive, anti-asthmatic and expectorant activities. J Ethnopharmacol 129(3), 293-8. PMID: 20381600.

Stanojevic L, Stanojevic M, Nikolic V, Nikolic L, Ristic J, Canadano Vic-Brunet J, Tumbas V. 2009. Antioxidant activity and total phenolic and flavonoid contents of Hieraciumpilosella L extract, Sensors (Basel) 9(7), 5702-5714.

Sangeetha M, Preethi S. 2014. Qualitative and quantitative analysis of bioactive compounds in Morindacitrifolia fruit, Indian journal of advances in plant research 16, 5-8.

Sathyavathi GV, Gupta AK, Tandon N. 1987. Medicinal Plants of India 2, New Delhi, India: Indian Council of Medical Research.

Toshiya K, Testuya T, Akira H, Takuji T. 2012. Cancer chemoprevention through the induction of apoptosis by natural compounds”, Journal of Biophysical Chemistry 3, 156-173.

Tariq AL, Reyaz AL. 2013. Quantitative phytochemical analysis of traditionally used medicinal plant Terminaliachebula, International Research Journal of Biotechnology 4(5), 101-105.

WHO. 1978. The Promotion and development of traditional medicine. World Health Organization. Technical Report Series No. 622.2.