Prevalence of Eimeria bovis in cattles of Cholistan desert, Pakistan

Paper Details

Research Paper 01/08/2016
Views (737)
current_issue_feature_image
publication_file

Prevalence of Eimeria bovis in cattles of Cholistan desert, Pakistan

Syeda Rida Zainab, Mirza Imran Shahzad, Muhammad Zahid Mustafa, Maryam Arshad, Tahira Ruby
J. Biodiv. & Environ. Sci. 9(2), 94-98, August 2016.
Copyright Statement: Copyright 2016; The Author(s).
License: CC BY-NC 4.0

Abstract

Protozoan parasites are responsible for a number of diseases in livestock thus affecting a country economy. The coccidiosis is very common parasitic disease in livestock of Pakistan especially in desert animals. A valid procedure was used to detect E. bovisin fecal samples of cholistanicow. PCR reaction was performed using species specific primer for amplification of ITS-1 region of rRNA gene. E. bovis was confirmed in fecal samples obtained cholistani cow while E. zuernii was absent. The PCR based detection of Eimeria parasites seemed highly sensitive when compared with traditional morphological examination of oocytes.

Akhtar M, Anwar MI, Iqbal Z, Muhammad F, Awais MM, Haq AU, Sawicka HE. 2012. Immunological evaluation of two local isolates of Eimeria tenella gametocytes against coccidiosis in poultry. Pakistan veterinary Journal 32, 77-80. www.pvj.com.pk/abstract/32_1/11-141.htm.

Ali I, Chauhdry MS, Farooq U. 2009. Camel rearing in Cholistan desert of Pakistan. Pakistan veterinary Journal.  29, 85-92.

Costa CA, Gomes RF, Melo MN, Ribeiro MF. 2001. Eimeria parasite of domestic fowl, genetic relationship of different isolates estimated from RAPD-DNA. Parasitology Research 87, 459-456. www.agencia.cnptia.embrapa.br

Hoge CW, Shlim DR, Ghimire M. 1995. Placebo-controlled trial of cotrimoxazole for cyclospora infections among travellers and foreign residents in Nepal. Lancet 345, 691-693. www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC).

Kausiluka LJM, Kambarage DM, Matthewman RW, Harrison LHS, Daborn CJ. 1996. Coccidiosis in small ruminants in Tanzania. Small Ruminant Research 21, 127-131. www.sciencedirect.com/science/article/pii/0921448896008607.

Kawahara F, Taira K, Nagai S, Onaga H, Onuma M, Nunoya T. 2008. Detection of five avian Eimeria species by species-specific realtime polymerase chain reaction assay. Avian Diseases 52, 652-656. www.ncbi.nlm.nih.gov/pubmed/ 19166058.

Kawaharaa F, Zhanga G, Mingalab CN, Tamurac Y, Koiwac M, Onumaa M, Nunoyaa T. 2010. Genetic analysis and development of species-specific PCR assays based on ITS-1 region of rRNA in bovine Eimeria parasites. Veterinary Parasitology 174, 49-57. www.researchgate.net/46148767.

Khan IA, Khan A, Hussain A, Riaz A, Aziz A. 2011. Hemato-biochemical alterations in crossbred cattle affected with bovine theileriosis in semiarid zone. Pakistan Veterinary Journal 31, 137-140. www.researchgate.net/50828633.

Levine ND, Ivens V. 1970. The Coccidian Parasites (Protozoa, Sporozoa) of Ruminants. Illinois Biology Monograph No. 44. University of Illinois Press, London.  www.ideals.illinois.edu/handle/2142/26596.

Li G, Xiao S, Zhou R, Li W, Wadeh H. 2007. Molecular characterization of Cyclospora-like organism from dairy cattle. Parasitology Research 100, 955-961. www.ncbi.nlm.nih.gov/pubmed/17206510.

Martynova VA, Syvyk A, Teplova I, Hume M, Nalian A. 2010. Rapid detection of avian Eimeria species using denaturing gradient gel electrophoresis. Veterinary Parasitology 174, 49-57. www. researchgate.net/23220037.

Nalbantoglu S, Sari B, Cicek H, Karaer Z. 2008. Prevalence of coccidian species in the water buffalo (Bubalusbubalis) in the Province of Afyon, Turkey. Acta Veterinaria Brno 77, 111-116.actavet. vfu.cz › Archive › 2008, Vol. 77 › Issue 1.

Olsen GJ, Woese CR. 1993. Ribosomal RNA: a key to phylogeny. FASEB J 7, 113-123. www.ncbi.nlm.nih. gov/pubmed/8422957.

Om H, Kumar S, Singh P. 2010. Prevalence of coccidia in Mathura region of Uttar Pradesh. Veterinary World 3, 503-505. www.scopemed.org/mno=2157.

Raza MA, Younus M, Schlecht E. 2014. Prevalence of gastrointestinal helminths in pastoral sheep and goat flocks in the Cholistan desert of Pakistan. Journal of Animal and Plant Sciences 24, 127-134. www.thejaps.org.pk/docs/v-24-1/19.pdf.

Schnitzler BE, Thebo PL, Mattsson JG, Tomley FM, Shirley MW. 1998. Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic Eimeria species of the chicken. Avian Pathology 27, 490-497. www.tandfonline.com/doi/pdf/10.1080/03079459808419373.

Schnitzler BE, Thebo PL, Tomley FM, Uggla A, Shirley MW. 1999. PCR identification of chicken Eimeriaa simplified read-out. Avian Pathology 28, 89-93. www.ncbi.nlm.nih.gov/pubmed/16147553.

Woods WG, Whithear KG, Richard DG, Anderson GR, Jorjensen WK, Gasser RB. 2000. Single strand RFLP analysis of ITS-2 fragment for six species of Eimeria from chickens in Australia. International Journalof Parasitology 30, 1019-1023. www.europepmc.org/abstract/med/10980293.

Related Articles

Dietary Aloe vera improves growth and hematology in Nile tilapia (Oreochromis niloticus)

Fatima Khan*, J. Biodiv. & Environ. Sci. 28(4), 89-99, April 2026.

Intercropping camphor basil shrubs with selected food crops for ecosystem services in the upper midland agroecological zone of Western Kenya

Reuben K. B. Chumba*, Alex Awiti, Francis Namasaka Muyekho, Vitalis Ogemah, Jacob Omollo, Yosef Kidane Gebrehawariat, J. Biodiv. & Environ. Sci. 28(4), 73-88, April 2026.

Surveillance and detection of African swine fever on abbatoir in different municipalities of third district of Cagayan, Philippines

Maricel F. Campanano, John Michael M. Melad, Mary Ann M. Santos*, J. Biodiv. & Environ. Sci. 28(4), 65-72, April 2026.

Mobile-based potato leaf disease identifier using ensemble modeling

Karen W. Cantilang*, Laarni M. Ladiao, J. Biodiv. & Environ. Sci. 28(4), 58-64, April 2026.

Diagnostic analysis of pig farms in the North of Côte d’Ivoire: Case of the commune of Korhogo

Seni Kouadio Sylvain*, Kadjo Vincent, Alla Konan Jean Bedel, Yao Koffi Sylvanus Aubert, N’glouan Wadjé Jérôme, Soro Ouation Souleymane, Kouassi Koffi Dongo, J. Biodiv. & Environ. Sci. 28(4), 48-57, April 2026.

Coral reef condition in Illana Bay, Zamboanga del Sur, Philippines: Basis for conservation management

Ruel S. Lasagas, Rosanilio M. Yagos*, Edgardo H. Rosales, J. Biodiv. & Environ. Sci. 28(4), 40-47, April 2026.

Preliminary floral and faunal species diversity in Maluyo River in Santol, La Union, Philippines

Judith M. Morales*, Analyn V. Sagun, Angelina T. Gonzales, J. Biodiv. & Environ. Sci. 28(4), 26-39, April 2026.

Challenges and impact of the farmer-scientists training program on community development in Bohol, Philippines

Jeffrey O. Awas*, Anabel J. Intong, Aida T. Salingay, Manolito C. Macalolot, J. Biodiv. & Environ. Sci. 28(4), 8-25, April 2026.