Probing the antioxidant status and total phenolic contents of sweet basil seeds and its functional drink

Paper Details

Research Paper 01/05/2019
Views (245) Download (9)

Probing the antioxidant status and total phenolic contents of sweet basil seeds and its functional drink

Muhammad Tahir, Muhammad Tahir Nadeem, Muhammad Umair Arshad, Tanvir Ahmad, Farhan Saeed, Ahsan Javed, Muhammad Nouman
Int. J. Biosci.14( 5), 440-449, May 2019.
Certificate: IJB 2019 [Generate Certificate]


In the modern era, nutritionists are determined to improve public health and discover particular innovative approaches for subsiding the lifestyle related disorders. Diet based therapy is considered as most effective owing to its health promoting perspectives. Keeping in this view the present study was planned to characterize sweet basil seeds (Lallemantia royleana), for antioxidant activity and total phenolic contents. For the purpose, raw material (Lallemantia royleana seeds) was procured from local market, Faisalabad and bioactive moieties were extracted with n-hexane, methanolic and ethanolic solvents from them. In addition, chemical characterization was carried out through respective methods. Furthermore, anti-oxidant activity of methanolic seed extract as well as drink prepared with  the extracts of L. royleana were ascertained in vitro by super oxide scavenging activity by DPPH free radical scavenging activity, TPC and FRAP. Compositional analysis elucidated that sweet basil seeds are good sources of fiber (4.87 %) and protein (22.40 %). The results for extraction depicted high extraction yield in methanolic extracts 1.81g/100g followed by ethanol 1.42g/100g and n-hexane 1.13g/100g. Moreover, Sweet basil seed extract contained DPPH (9.47mg/ml) and FRAP (15.69mM FRAP/g). Furthermore, results regarding DPPH (2.50±0.04 to 2.31±0.59) FRAP (4.22±0.07 to 4.01±0.05) and TPC (4.09±0.97 to 2.22±0.66) showed gradual decrease on the storage ability of the drink. Rooted from study, it can be concluded that the plant extract should be used for the treatment of numerous aliments caused by oxidation.


AACC. 2000. Approved Methods of Analysis. Methods. St. Paul, MN, U.S.A: American Association of Cereal Chemists International.

AOAC. 1990. Association of official, chemists, official methods of analysis. 15th Edition, Washington DC, U.S.A. ai medicinal plant. International Journal of Systematic and Evolutionary Microbiology 58, 2439-2442.

Bozin B, Mimica-Dukic N, Simin N,  Anackov G. 2006. Characterization of the volatile composition of essential oils of some Lamiaceae spices and the antimicrobial and antioxidant activities of the entire oils. Journal of agricultural and food chemistry 54(5), 1822-1828.

El-Beshbishy H, Bahashwan S. 2012. Hypoglycemic effect of basil (Ocimum basilicum) aqueous extract is mediated through inhibition of α-glucosidase and α-amylase activities: an in vitro study. Toxicology and Industrial Health 28(1), 42-50.

Fatima J, Mansoor S, Saadia MA, Rehman M, Mustafa H. 2016. Phyto-Chemical Analysis, In-Vitro Antioxidant Potential and GC-MS of Lallemantia royleana Seeds. International Journal of Scientific and Research Publications 6(2).

Hegde K, Joshi AB. 2010. Hepatoprotective and anti-oxidant effect of Carissa spinarum root extract against CCl4 and paracetamol-induced hepatic damage in rats. Bangladesh Journal of Pharmacology 5(1), 73-76.

Hinneburg I, Dorman HD, Hiltunen R. 2006. Antioxidant activities of extracts from selected culinary herbs and spices. Food chemistry 97(1), 122-129.

Latimer GW. 2012. Official methods of analysis of American Association of Cereal Chemists International.

Mahmood S, Hayat MQ, Sadiq A, Ishtiaq S, Malik S, Ashraf M. 2013. Antibacterial activity of Lallemantia royleana (Benth.) indigenous to Pakistan. African journal of microbiology research 7(31), 4006-4009.

Mir MA, Sawhney S, Jassal M. 2013. Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale. Wudpecker Journal of Pharmacy and Pharmocology 2(1), 1-5.

Moghaddam TM, Razavi SM, Emadzadeh B. 2011. Rheological interactions between Lallemantia royleana seed extract and selected food hydrocolloids. Journal of the Science of Food and Agriculture91(6), 1083-1088.

Muller FL, Lustgarten MS, Jang Y, Richardson A, Van Remmen H. 2007. Trends in oxidative aging theories. Free Radical Biology and Medicine 43(4), 477-503.

Nurzynska-Wierdak R, Borowski B, Dzida K, Zawislak G, Kowalski R. 2013. Essential oil composition of sweet basil cultivars as affected by nitrogen and potassium fertilization. Turkish Journal of Agriculture and Forestry 37(4), 427-436.

Pirani A, Moazzeni H, Mirinejad S, Naghibi F, Mosaddegh M. 2011. Ethnobotany of Juniperus excelsa M. Bieb.(Cupressaceae) in Iran. Ethnobotany Research and Applications 9, 335-341.

Prashant T, Bimlesh K, Mandeep K, Gurpreet K, Harleen K. 2011. Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia 1(1), 98-106.

Rafe A, Razavi SM, Farhoosh R. 2013. Rheology and microstructure of basil seed gum and β-lactoglobulin mixed gels. Food Hydrocolloids 30(1), 134-142.

Razavi SM, Mortazavi SA, MatiaMerino L, HosseiniParvar SH, Motamedzadegan A,  Khanipour E. 2009. Optimisation study of gum extraction from Basil seeds (Ocimum basilicum L.). International journal of food Science & Technology 44(9), 1755-1762.

Razavi SMA, Moghaddam TM, Amini AM. 2008. Physical-mechanical properties and chemical composition of Balangu (Lallemantia royleana (Benth. in Walla.) seed. International journal of food engineering 4(5).

Shweta M, Akanksha B, Nayyar P, Pramod K. 2016. Extraction and use of lallemantia royleana seed mucilage as a pharmaceutical excipient. World Journal of Pharmaceutical Research 4(4), 1578-1589.

Singleton VL, Orthofer R, Lamuela-Raventós RM. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent Methods in enzymology 299, p 152-178.

Steel RGD, Torrie JH. 1997. Principles and procedures of statistics, a biometrical approach: McGraw-Hill Kogakusha, Ltd.

Sun C Fu J, Chen J, Jiang L, Pan Y. 2010. On‐line HPLC method for screening of antioxidants against superoxide anion radical from complex mixtures. Journal of separation science 33(8), 1018-1023.

Taga MS, Miller E, Pratt D. 1984. Chia seeds as a source of natural lipid antioxidants. Journal of the American Oil Chemists’ Society 61(5), 928-931.

Wangcharoen W, Morasuk W. 2007. Antioxidant capacity and phenolic content of holy basil. Songklanakarin Journal of Science and Technology 29(5), 1407-1415.

Yuan F, Quataert E, Narayan R. 2003. Nonthermal electrons in radiatively inefficient accretion flow models of Sagittarius A. The Astrophysical Journal 59(1), 301.