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Production of lipases by Alternaria sp. (mbl 2810) through optimization of environmental conditions using submerged fermentation technique

Tehreema Iftikhar, Roheena Abdullah, Mehwish Iqtedar, Afshan Kaleem, Mahwish Aftab, Mubashir Niaz, Sidra, Bushra Tabassum, Hammad Majeed

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Int. J. Biosci.7(2), 178-186, August 2015

DOI: http://dx.doi.org/10.12692/ijb/7.2.178-186


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The present investigation was conducted for the biosynthesis of extracellular and intracellular lipases by Alternaria sp. (MBL 2810) through submerged fermentation technique. To increase the lipases production various parameters were optimized such as nitrogen, carbon sources and additional additives besides the basic cultural conditions e.g., rate of fermentation, volume of fermentation medium, inoculum size and type and initial pH of fermentation medium. The maximum extracellular and intracellular lipases production was achieved, when fermentation was optimized at room temperature for 24 h at the agitation speed of 100 rpm. Other cultural conditions such as inoculum type and inoculum size (4.0 mL) was also optimized, glycine as an additional carbon source and urea as an additional nitrogen source gave the maximum extracellular lipases activity. Maximum production of extracellular lipases (9.04 ± 0.05a U/mL) was achieved when volume of fermentation was optimized at 25mL and maximized intracellular lipases (10.04 ± 0.13a U/mL) production was achieved when the medium pH was adjusted at 5.


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Production of lipases by Alternaria sp. (mbl 2810) through optimization of environmental conditions using submerged fermentation technique

Babu IS, Rao GH. 2007. Lipase production by Yarrowia lipolytica NCIM 3589 in solid state fermentation using mixed substrate. Research Journal of Microbiology 2, 469–474.

Banjamin S, Panday A. 1997. Coconut cake: a potent substrate for production of lipase by Candida sugasa in solid state Acta. Biotecnologica 17, 241-51.

Chahinian H, Vanot G, Ibrik A, Rugani N, Sarda L, Comeau LC. 2000. Production of acylglycerol lipase by Penicillium cyclopium purification and characterization of partial acylglycerol lipase. Bioscience, Biotechnology Biochem 64, 215-222.

Cihangir N, Sarikaya E. 2004. Investigation of lipase production by a new isolated of Aspergillus sp. World Journal of Microbiology and Biotechnology 20, 193–197.

Corzo G, Revah S. 1999. Production and characteristics of the lipase from Yarrowia lipolytica 681. Bioresource Technology 70,173-80.

Freire DMG, Teles EMF, Bon EPS, Anna GLS. 1997. Lipase production Penicillium restrictum in a Bench-scale fermenter: Effect of carbon and nitrogen nutrition, agitation and aeration. Applied Biochemistry and Biotechnology 63, 409-421.

Ghorai S, Banik SP, Verma D, Chowdhury S, Mukherjee S, KH S. 2009. Fungal biotechnology in food and feed processing. Food Research International 42, 577-587.

Gupta N, Sahaii V, Gupta R. 2007. Alkaline lipase from a novel strain Burkholderia multivorans. Process Biochemistry 9, 516-526.

Haq I, Sultan MA, Adnan A, Qadeer MA. 1988. Studies on the production of lipases by different mould cultures. Biologia 44, 66-78.

Hiol A, Jonzo MD, Rugani N, Druet D, Sardo L, Comeau LC. 2000. Purification and characterization of an extracellular lipase from a thermophilic Rhizopus oryzae strain isolated from palm fruit. Enzyme and Microbial Technology 26, 421-430.

Iftikhar T, Niaz M, Afzal M, Haq IU, Rajoka MI. 2008. Maximization of intracellular lipase production in a lipase overproducing mutant derivative of Rhizopus oligosporus DGM 31: A kinetic study. Food Technology and Biotechnology 46, 402-412.

Jaeger KE, Eggert T. 2002. Lipases for biotechnology. Current Opinion in Biotechnology 13, 390-397.

Kirk O, Borchert TV, Fuglsang CC. 2002. Industrial enzyme applications. Current Opinion in Biotechnology 13, 345-351.

Kundu AK, Pal N. 1970. Isolation of lipolytic fungi from the soil. Journal Pharmacy India 132, 96-97.

Lawrence BC, Mitchell TK, Craven KD, Cho Y, Cremer RA, Kim KH. 2008. At Death’s Door: Alternaria Pathogenicity Mechanisms. The Plant Pathology Journal 24, 101-111.

Li D, Wang B, Tan T. 2006. Production enhancement of Rhizopus arrhizus lipase by feeding oleic acid. Journal of Molecular Catalysis B: Enzymatic 43, 40–43.

Maritenelle M, Holmquist M, Hult K. 1995. On the interfacial activation of Candida Antarctica lipase a and b compared with Humicola lanoginosa lipase. Biochimica et Biophysica Acta (BBA) – Lipids and Lipid Metabolism 1258, 272-276.

Martinez C, Geus PD, Stanssens P, Lauwereys M, Cambillau C. 1993. Engineering cysteine mutants to obtain crystallographic phases with a cutinas from Fusarium solani pisi. Protein Engineering 6, 157-165.

Mladenoska I, Dimitrovski A. 2001. Lipase production by geotrichum candidum-m2. Bullet Chem. Technol. Mace, 20, 39-43.

Moataza M, Amany SAAD, Kansoh L, Gadallah MA. 2004. Optimization of extracellular lipase production by Fusarium oxysporum. Arabaian Journal Biotech 8, 19-28.

Morais JR, Ledingham AM, Filho LLJ. 2001. Effect of culture conditions on lipase production by Fusarium solani in batch fermentation. Bioresource Technology 76, 23-27.

Nahara H, Koyama Y, Yoshida T. 1982. Growth and enzyme production in solid state culture of Aspergillus oryzae. Journal of Fermentation Technology 60, 311-319.

Panday A, Benjamin S, Soceol CR, Nigam P, Krieger N, Socol UT. 1999. The realm of microbial lipases Biotechnology. Biotechnology and Applied Biochemistry 29, 119-131.

Papagiani M. 2014. An evaluation of proteolytic and lipolytic potential of Penicillium sp. isolated from traditional Greeks sausages in submerged fermentation. Applied Biochemistry and Biotechnology 172, 767-775.

Papagiauni M, Nokes SE, Filer K. 2001. Submerged and Solid –state phytase fermentation by Aspergillus niger. Effect of agitation and medium viscosity and inoculum performance. Food Technol. Biotechnology 35(6), 397-402.

Riffat HM, El-Mahalawy AA, El-Menofy HA, Donia SA. 2010. Production, Optimization and partial purification of lipase from Fusarium oxysporum. Journal of Applied Sciences in Environmental Sanitation 5, 39-53.

Sangeetha R, Geetha A, Arulpandi I. 2008. Optimization of protease and lipase production by Bacillus pumilus SG 2 isolated from an industrial effluent. International Journal of Microbiology 5, 2.

Sharma R, Chisti Y, Banergee UC. 2001. Production, purification, characterization and applications of lipases. Biotechnology Advances 19, 627-662.

Sztajer H, Maliszewska I, Wieczorek J. 1988. Production of exogenous lipase by bacteria, fungi and actinomycetes. Enzyme and Microbial Technology 10, 492–7.

Thomma BPHJ. 2003. Alternaria spp. from general saprophyte to specific parasite. Molecular Plant Pathology 4(4), 225-236.

Wang Y, Srivastava KC, Shen GJ, Wang HY. 1995. Thermostable alkaline lipase from a newly isolated thermophilic Bacillus strain, A30-1 (ATCC 53841). Journal of Fermentation and Bioengineering 79, 433.


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