Production of pectinase by Aspergillus niger cultured in solid state media
Paper Details
Production of pectinase by Aspergillus niger cultured in solid state media
Abstract
Solid state fermentation was carried out with 7 fungal strains, obtained from different sources. Among 7 isolates Aspergillus niger, IM-6 was found as effective pectinase producer. Maximum enzymatic activity (142.44U/gm) was observed after 7 days incubation at 40˚C temperature in 750 ml conical flask. In this study 1.69% (NH4)2SO4 was used as nitrogen source, although peptone as a nitrogen source showed better result but use of peptone was not cost effective. As a substrate, wheat bran and potato starch showed good result (85.54U/gm) in solid state culture. Addition of 9.68% pectin was found to increase the enzyme production as 116.57U/gm. Pectinase production was optimum in 60% moisture (98.34U/gm). Aeration showed positive effects on pectinase production (136.86U/gm) at 750 ml flask than 1000 ml flask. Thus the wild strain Aspergillus niger IM-6 has outstanding pectinase producing capability at 40◦C in 60% initial moisture content for 7 days of incubation in solid state fermentation.
Abe J, Bergman FW, Obata K, Hikuri S. 1988. Production of raw starch digesting amylase by Aspergillus K-27. Applied Microbiology and Biotechnology 27, 447-450
Aguilar G, Huitron C. 1987. Stimulation of the production of extracellular pectinolytic activities of Aspergillus sp. by galactouronic acid and glucose addition. Enzyme Microb. Technol 9, 690-696.
Venugopal C, Jayachandra T, Appaiah KA. 2007. Effect of aeration on the production of Endo-pectinase from coffee pulp by a novel thermophilic fungi Mycotypha sp. Strain No. AKM1801 6(2), 245-250.
Dekker RFH, Barbosa AM. 2001. The effect of aeration and veratryl alcohol on the production of two laccases by the ascomycete Botryosphaeria sp. Enzyme and Microbial. Technology 28, 81–88.
Eric O, Maurice R, Sevastianos R, Gustavo V. 1988. Water and water activity in the solid state fermentation of cassava starch by Aspergillus rtiger. Applied Microbiology and Biotechnology 27, 498-503.
Fujio Y, Eledago FB. 1993. Polygalacturonase production by Rhizopus species. Journal of General and. Applied. Microbiology 39, 409-418.
Itoh Y, Sugiura J, Izaki K, Takahashi H. 1982. Enzymological and immunological properties of pectin lyases from bacteriocinogenic strains of Erwinia carotovora Agricultural and Biological Chemistry 46, 199-205.
Kitpreechavanich V, Hayashi M, Nagai S. 1984. Productionof xylan-degrading enzymes by thermophillic fungi, Aspergillus fumigatus and Humicola lanuginosus. Journal of Fermentation Technology 62, 63-69.
Lonsane BK, Ramesh MV. 1992. Production of bacterial thermostable enzymes by solid state fermentation: a potential tool for achieving economy in enzyme production. Advance Applied Microbiology 15, 1-48
Marie KWS, Kevin DH, Stephen BP. 2002. Comparative Enzyme Production by Fungi from Diverse Lignocellulosic Substrates. The Journal of Microbiology 40(3), 241-244
Pereira SS, Torres ET, Gonzalez GV, Rojas MG. 1992. Effect of different carbon sources on the synthesis of pectinase by Aspergillus niger in submerged and solid state fermentation. Applied Microbiology and Biotechnology 39, 36-41.
Perrone G, Mulè G, Susca A, Battilani P, Pietri A, Logrieco A. 2006. Ochratoxin A production and AFLP analysis of Aspergillus carbonarius, Aspergillus tubingensis, and Aspergillus niger strains isolated from grapes in Italy. Applied and Environmental Microbiology 72, 680 -685
Sakai T, Okushima M, Sawada M. 1982. Some properties of endo-polygalacturonase from Trichosporon penicillatum SNO-3. Agricultural and Biological Chemistry 46, 2223-2231.
Stiles HR, Peterson WH, Fred EB. 1926. A rapid method for the determination of sugar in bacterial cultures. Journal of Bacteriology 12(6), 427-430.
Tjamos SE, Antoniou PP, Kazantzidou A, Antonopoulos DF, Papageorgiou I, Tjamos EC. 2004. Aspergillus niger and Aspegillus carbonarius in Corinth raisin and wine-producing vineyards in Greece: population composition, ochratoxin A production and chemical control. Journal of Phytopathology 152, 250 -255
Trejo-Hernendez MR, Oriol E, Lopez-Canales A, Roussos S, Viniegra G, Raimbault M. 1991. Production of pectinase by Aspergillus niger by solid state fermentation on support. Micol Neotrop Apl 4, 49-62.
Yamasaki M, Yashui T, Arima K. 1964. Pectic enzymes in the clarification of apple juice Part 1. Study on the clarification reaction in a simplified model. Agriculture and Biological Chemistry 28(11), 779-787.
Nazneen Akhter, M Alam Morshed, Azim Uddin, Feroza Begum, Tipu Sultan, Abul Kalam Azad (2011), Production of pectinase by Aspergillus niger cultured in solid state media; IJB, V1, N1, February, P33-42
https://innspub.net/production-of-pectinase-by-aspergillus-niger-cultured-in-solid-state-media/
Copyright © 2011
By Authors and International
Network for Natural Sciences
(INNSPUB) https://innspub.net
This article is published under the terms of the
Creative Commons Attribution License 4.0