Protective effect of Asparagus adscendens on 5-fluorouracil induced gastrointestinal lesions in Wistar rats

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Research Paper 05/05/2024
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Protective effect of Asparagus adscendens on 5-fluorouracil induced gastrointestinal lesions in Wistar rats

Diksha Khajuria, Nawab Nashiruddullah, Nrip Kishore Pankaj, Raja A.H Kuchay, Jafrin Ara Ahmed, Syedah Asma Andrabi, Dibyendu Chakraborty, A.A. Shah
Int. J. Biosci.24( 5), 117-125, May 2024.
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The use of the anti-cancer drug 5-fluorouracil (5-FU) is known to induce intestinal mucositis in patients without known satisfactory preventive therapy. An ethanolic extract of Asparagus adscendens (A. a. EE) of was prepared with extractability percentage calculated as 27.2% (w/w) anda simple solution was made using CMC (carboxy methyl cellulose).Twenty-four five-week-old male Wistar rats were divided in four groups- Group-I acted as  control(receiving oral saline), Group-II receiving 5-FU, Group-III receiving 5-FU and Asparagus adscendens EE, and lastly Group-IV receiving Asparagus adscendens EE alone. Dosage of Asparagus adscendens EE (300mg/kg body weight) was prepared in three divided doses and administered by gavage to the rats. A single intraperitoneal dose of 5-FU (500 g/ml) was injected in the test groups II and III. A drastic and significant (p<0.01) fall in body weight between Group-II and III rats, together with severe diarrhoea score in these groups was noted. The histopathological scores derived from 11 devised parameters showed a severe and significant (p<0.01) affliction of the ileum as compared to the jejunum in Groups-II and III. With 5-FU administration, histopathological changes in the jejunum were associated with severe pathology, such as- villi shortening, disruption of tips, degenerate enterocytes, Goblet cell reduction, marked mucosal mononuclear cell infiltration, significant loss of crypts or their distortion/loss, congestion of blood vessels and submucosal oedema. Similar changes also appeared in the ileum, together with lacteal dilation and hyperplastic Peyer’s patches. In Group-III, enteric changes in the jejunum and ileum were indicative of mild enteritis, although far less in severity than observed in Group-II. In Group-IV changes were normal except for abundant Goblet cells, both in the jejunum and ileum. Overall Asparagus adscendens was found to significantly improve the detrimental effects induced by 5-FU.


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