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Protective effect of selenium supplementation on antioxidant defence and cardiovascular diseases in alloxan diabetic rats

Research Paper | March 1, 2014

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S.Boussekine, N.Bouzerna, R.Rouabhi

Key Words: Selenium, Diabetes, Cardiovascular diseases, Oxidative stress, Rats, Alloxan

Int. J. Biosci.4( 5), 1-10, March 2014

DOI: http://dx.doi.org/10.12692/ijb/4.5.1-10


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To investigate the protective effect of selenium supplementation in the cardiovascular diseases associated with diabetes mellitus caused by lipid peroxidation and oxidative stress. Thirty five male albino (wistar) rats of 06 weeks of age were randomly divided into five groups of seven each. Two groups were pre-treated for 10 days by sodium selenite (2 mg/kg.bw/d) via orogastric route. The first group was served as the control, the second as the normal treated intra peritoneally by selenium (1.89 mg/kg.bw/d). Three groups were intra peritoneally injected with alloxan (150 mg/kg.bw) to induce diabetes. One diabetic group treated intra peritoneally by insulin (3UI/100g. bw), another diabetic group (pre-treated by selenium 2 mg/kg.bw/d for 10 days) treated intra peritoneally by selenium (1.89 mg/kg.bw/d) for further three weeks. The administration of alloxan significantly increased blood glucose, total cholesterol, LDL-C, triglycerides, lipids, heart MDA content, GST activity. In contrast blood HDL-C, insulin levels, G6PDH activity, heart GSH, GPx, Catalase activities were significantly decreased. Supplementation of sodium selenite restores the parameters measured compared to untreated diabetic rats and diabetic treated by insulin. To conclude the present study shows that treatment of diabetic rats with sodium selenite prevented cardiovascular diseases induced by hyperlipemia, lipid peroxidation and free radicals generated during diabetes.


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