Purification and characterization of amyloglucosidase produced by a mutant strain of Aspergillus Niger

Paper Details

Research Paper 01/11/2016
Views (281) Download (7)

Purification and characterization of amyloglucosidase produced by a mutant strain of Aspergillus Niger

Shazia Malik, IkramulHaq, Tehreema Iftikhar
Int. J. Biosci.9( 5), 108-115, November 2016.
Certificate: IJB 2016 [Generate Certificate]


Amyloglucosidases, are the enzymes which releases glucose by hydrolyzing starch and oligosachharides as it hydrolyzes µ-1, 4 and µ-1, 6 linkages of the saccharides formed by the action of other amylases on starch and has great importance in the starch industries. The present study is concerned with the partial purification of amyloglucosidase produced by the mutant strain of Aspergillusniger using ammonium sulphate precipitation method and characterization of the enzyme. The maximum activity of amyloglucosidase was achieved after 60 min of incubation when maintained at pH 4.75 and temperature of 60oC with 5% starch concentration.


Arassaratnam KT, Vasanthy Z, Muragapoopthy, Thiagarajah JK, Balasubramanium R, Kandiah S. 1994. Effect of pH on preparation and performance of physically immobilized amyloglucosidase on DEAE cellulose. Starch/Staerke  Eng  46(4), 1467-1469.

Arassaratnam V, Mylvagunam K, Balasubramanian T. 1997. Paddy husk support and rice bran for production of glucoamylase by Aspergillus niger. J. Food Sci. Technol  32(4), 299-304.

Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry  72, 248-254.

Costa JAV, Colla E, Magagnin G, Santos LO, Vendruscolo M, Bertolin TE. 2007. Simultaneous amyloglucosidase and exopolygalacturonase production by Aspergillus niger using solid state fermentation. Braz Arch Boil Technol 50(5), www.dx.doi.org/10.1590/S151689132007000500003

Deutscher MP. 1990. Guide to protein purification. Methods in enzymology 182, 285-295.

Dilera G, Chevalliera S, Pöhlmanna I, Guyona C, Guillouxa M, Le-Baila A. 2015. .Assessment of amyloglucosidase activity during production and storage of laminated pie dough. Impact on raw dough properties and sweetness after baking J of Cereal Science  61, 63–70.

Fogarty WM, Benson CP. 1983. Purification and properties of a thermophilicamyloglucosidase from Aspergillus niger. J Appl Microbiol Biotechnol  18(5), 271-278.

Ford C. 1999. Improving operating performance of glucoamylase by mutagenesis. Curr Opin Biotechnol 10,  352–357.

Haq I, Ashraf H, Omar S, Qadeer MA. 2002. Biosynthesis of amyloglucosidase by Aspergillus niger using wheat bran as substrate. Pak. J  of Biol  Sci  5(9),  962-964.

Hyun HH, Zeikas JC. 1985. General biochemical characterization of Thermostable pollulanase and glucoamylase from Clostridium thermohydrosufuricum. Appl  Environ  Microbiol  49(5), 1168-1173.

Kelly CT, Reilly FO, Fogarty WM. 1983. Extracellular alpha-glucosidase of an alkalophilic microorganism, Bacillus spp ATCC 21591. Microb Lett 20, 55-59.

Khalaj V, Brookman JL, Robson GD. 2001. A study of the protein secretary pathway of Aspergillus niger using a glucoamylase-GFP Fusion protein. Fungal Genet. Biol 32(1), 55-65.

Laemmli UK. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680-685.

Malik S, Iftikhar T, Haq IU. 2011. Enhanced amyloglucosidase biosynthesis through mutagenesis using Aspergillus niger. Pak  J.  Bot 43(1), 111-119.

Manera AP, Kamimura ES, Brites LM, Khalil SJ. 2008. Adsorption of Amyloglucosidase from Aspergillus niger NRRL 3122 using Ion Exchange Resin  Braz  Arch  Of Biol  Technol  51(5), 1015- 1024.

Omemu AM, Akpan I, Bankole MO, Teniola OD. 2005. Hydrolysis of raw tuber starches by amylase of Aspergillus niger AM07 isolated from the soil. Afric  J of Biotechnol  4(1), 19-25.

Omemu AM, Akpan I, Bankole MO. 2008. Purification and characterization of extracellular Amyloglucosidase from Aspergillusniger CA-19 by solid state fermentation. Res. J. Microbiol  3(3),  129-135.

Pandey, Radhakrishan S. 1993. The production of glucoamylase by Aspergillus niger  NCIM 1245. Process Biochem  8,  305-309.

Pavezzi  FC, Gomes E, Silva R. 2008. Production and characterization of glucoamylase from fungus Aspergillus awamori expressed in yeast Saccharomyces cerevisiae using different carbon sources. Braz J. Microbiol  39(1), www.dx.doi.org/10.1590/S151783822008000100024

Polakovic  M, Bryjak J.  2004. Modelling of potato starch saccharification by an Aspergillus niger glucoamylase. J. Biochem  Eng 18,  57–64.

Preda G, Boeriu C, Deretey E, Peter F. 1996. Characterization of an amyloglucosidase by Aspergillusniger 1C strain. Ser Chin Ind Ing Mediului 41(12), 35-42.

Reilly PJ. 1999. Protein engineering of glucoamylase to improve industrial performance – a review. Starch/Starke  51,  269–274.

Roe S. 2001. Protein purification techniques. 2nd edition. Oxford University press. 134-142.

Singh S. 2007. A text book of Enzymes. CBI Publishers. 1, 15-33.

Selva KP, Ashakumary L, Helen A, Pandey A. 1996. Purification and characterization of glucoamylase produced by Aspergillusniger in solid state fermentation. Appl  Microbiol  Lett 23(6), 403-408.

Spier MR, Woiciechowski AL, Vandenberghe LPS, Soccol CR. 2006. Production and characterization of Amylases by Aspergillus niger under solid state fermentation using Agro industrials products. International Journal of Food Engeenering  2(3), 1-20.

Tsekovak K, Vicheva A, Tzekova A. 1999. Enhanced thermostability of glucoamylase by Aspergillus niger. Microbiologia 50(7-8), 181-185.