Qualitative and quantitative evaluation of tannins in bark extracts of some indigenous plants of Pakistan

Paper Details

Research Paper 01/06/2018
Views (745)
current_issue_feature_image
publication_file

Qualitative and quantitative evaluation of tannins in bark extracts of some indigenous plants of Pakistan

Tahira Aziz Mughal, Uzma Abid, Zeb Saddiqe, Sofia Nosheen, Shazia Pervaiz
Int. J. Biosci. 12(6), 78-84, June 2018.
Copyright Statement: Copyright 2018; The Author(s).
License: CC BY-NC 4.0

Abstract

Plant barks contain more tannin than any other plant part. In the present study two extraction techniques were applied to extract tannins from bark of some indeginous plants of Pakistan. The tannins were extracted from the barks of plants by maceration & ultrasonic extracion. Folin-Ciocalteu method was conducted to measure the total phenolic content in bark extracts of some plants. Polyvinylpyrrolidone (PVPP) solution was used to bind the tannins, so that the non-tannins (NT) content value can be detected. Thus, the total tannins (TT) content was measured. Qualitative tests for the determination of the presence of polyphenols/tannins were also conducted. It was seen that highest tannin content was present in plant Cassia fistula whereas the lowest tannin content was present in Quercus dilatate.

Association of Official Analytical Chemists. Official Methods of Analysis of the Association of Official Agricultural Chemists, 1975, 12 ed. Ed. AOAC, 1,. Washington.

Bandar H, Hijazi A, Rammal H, Hachem A, Saad Z, Badran B. 2013. Techniques for the extraction of bioactive compounds from Lebanese Utricadioica. American Journal of Phytomedicine and Clinical Therapeutics1, 507-513.

British Pharmacopoeia. 1999. The Stationary Office: London, volume I.

European Pharmacopoeia. 2002. EDQM: France.

FAO/IAEA. 2000. Division of Nuclear Techniques in Food and Agriculture, Quantification of Tannins in Tree Foliage, Vienna.

Paulo S. Farmacopéia Brasileira. 1988, Atheneu.

Horn D, Ditter W. 1982. Chromatographic study of interactions between polyvinylpyrrolidone and drugs. Journal of Pharmaceutical Sciences 71,1021-1026.

Makkar HPS, Bluemmel M, Browy NK, Becker K. 1993. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods, Journal of the Science of Food and Agriculture61, 161–165.

Makkar HPS, Bluemmel M, Becker K. 1995. Formation of complexes between polyvinylpyrrolidone and polethylene glycol with tannins and their implications in gasproduction and true digestibility in in vitro techniques. British Journal of Nutrition73,897–913.

Musa AE, Gasmelseed GA. 2012. Characterization of Lawsonia inermis (Henna) as vegetable tanning material. Journal of Forest Products & Industries 1, 35-40.

Sadasivam S, Manickam A. 2008. Biochemical methods, 3 ed. New Age International Pvt. Ltd, New Delhi.

Sarker SD, Latif Z, Gray AI. 2005. Natural products isolation, Springer.

Soares LAL, Maia A, Oliveira AL, Petrovick, PR, Ortega GG.2006. Avaliação de Complexos Formadospor Catequinae Macromoléculas. Acta Farmacéutica Bonaerense 25, 10-6.

Tonon R, Grosso C, Hubinger M. 2011. Influence of emulsion composition and inlet air temperature on the microencapsulation of flaxseed oil by spray drying. Food Research International 44,282-289.

Ukoha PO, Cemaluk EAC, Nnamdi OL, Madus EP. 2011. Tannins and other phytochemical of the Samanaea saman pods and their antimicrobial activities. African Journal of Pure and Applied Chemistry. 5, 237-244.

Verza SG, Kreinecker MT, Reis V, Henriques AT, Ortega GG. 2007. Evaluation of analytical variables of the Folin-Ciocalteu method for the quantitation of the total tannins content using a Psidium guajava L. leaves aqueous extract as a model. Química Nova 30, 815-820.

Related Articles

Integrated in silico and in vitro analyses reveal E-cadherin crosstalk and TF: FVIIa complex-mediated trophoblast motility via MEK/JNK activation

Kirthika Manoharan, Jagadish Krishnan, Vijaya Anand Arumugam, Shenbagam Madhavan*, Int. J. Biosci. 27(6), 136-144, December 2025.

Effect of flooding depth and harvest intensity on soil moisture dynamics and production of baobab (Adansonia digitata) seedlings

Sissou Zakari, Pierre G. Tovihoudji, Mouiz W. I. A. Yessoufou, Sékaro Amamath Boukari, Vital Afouda, Imorou F. Ouorou Barrè, Int. J. Biosci. 27(6), 127-135, December 2025.

Local food processing and associated hygienic quality in greater Lomé, Togo: Traditional cooked corn-based dough akpan wrapped in M. cuspidata, M. mannii and M. purpurea species leaves

Mamy Eklou, Komlan Edjèdu Sodjinou, Kodjo Djidjolé Etse, Awidèma Adjolo, Benziwa Nathalie Johnson, Bayi Reine Dossou, Yaovi Ameyapoh, Raoufou Radji, Akossiwoa M-L Quashie, Int. J. Biosci. 27(6), 114-126, December 2025.

Improving the microbiological quality of spices and spice blends using treatments accessible to SMEs/SMIs

Pingdwindé Marie Judith Samadoulougou-Kafando, Korotimi Traoré, Crépin Ibingou Dibala, Aboubacar Sidiki Dao, Josias Nikiema, Idrissa Taram, Adama Pare, Inoussa Salambéré, Donatien Kaboré, Charles Parkouda, Int. J. Biosci. 27(6), 102-113, December 2025.

Twin-row planting practice in village sugarcane (Saccharum officinarum L.) plantations during first ratoon under rainfed conditions in northern Côte d’Ivoire

Allé Yamoussou Joseph, Sawadogo Fatima, Traoré Mohamed Sahabane, Fondio Lassina, Int. J. Biosci. 27(6), 91-101, December 2025.

Prevalence of dengue infection in Delta State, Nigeria

P. A. Agbure, O. P. G. Nmorsi, A. O. Egwunyenga, Int. J. Biosci. 27(6), 82-90, December 2025.

Evaluation of silage quality of three sorghum varieties using in livestock systems of Burkina Faso

Barkwendé Jethro Delma, François Tapsoba, Nabèrè Ouattara, Gildas Marie Louis Yoda, Int. J. Biosci. 27(6), 72-81, December 2025.