Qualitative and quantitative evaluation of tannins in bark extracts of some indigenous plants of Pakistan

Paper Details

Research Paper 01/06/2018
Views (908)
current_issue_feature_image
publication_file

Qualitative and quantitative evaluation of tannins in bark extracts of some indigenous plants of Pakistan

Tahira Aziz Mughal, Uzma Abid, Zeb Saddiqe, Sofia Nosheen, Shazia Pervaiz
Int. J. Biosci. 12(6), 78-84, June 2018.
Copyright Statement: Copyright 2018; The Author(s).
License: CC BY-NC 4.0

Abstract

Plant barks contain more tannin than any other plant part. In the present study two extraction techniques were applied to extract tannins from bark of some indeginous plants of Pakistan. The tannins were extracted from the barks of plants by maceration & ultrasonic extracion. Folin-Ciocalteu method was conducted to measure the total phenolic content in bark extracts of some plants. Polyvinylpyrrolidone (PVPP) solution was used to bind the tannins, so that the non-tannins (NT) content value can be detected. Thus, the total tannins (TT) content was measured. Qualitative tests for the determination of the presence of polyphenols/tannins were also conducted. It was seen that highest tannin content was present in plant Cassia fistula whereas the lowest tannin content was present in Quercus dilatate.

Association of Official Analytical Chemists. Official Methods of Analysis of the Association of Official Agricultural Chemists, 1975, 12 ed. Ed. AOAC, 1,. Washington.

Bandar H, Hijazi A, Rammal H, Hachem A, Saad Z, Badran B. 2013. Techniques for the extraction of bioactive compounds from Lebanese Utricadioica. American Journal of Phytomedicine and Clinical Therapeutics1, 507-513.

British Pharmacopoeia. 1999. The Stationary Office: London, volume I.

European Pharmacopoeia. 2002. EDQM: France.

FAO/IAEA. 2000. Division of Nuclear Techniques in Food and Agriculture, Quantification of Tannins in Tree Foliage, Vienna.

Paulo S. Farmacopéia Brasileira. 1988, Atheneu.

Horn D, Ditter W. 1982. Chromatographic study of interactions between polyvinylpyrrolidone and drugs. Journal of Pharmaceutical Sciences 71,1021-1026.

Makkar HPS, Bluemmel M, Browy NK, Becker K. 1993. Gravimetric determination of tannins and their correlations with chemical and protein precipitation methods, Journal of the Science of Food and Agriculture61, 161–165.

Makkar HPS, Bluemmel M, Becker K. 1995. Formation of complexes between polyvinylpyrrolidone and polethylene glycol with tannins and their implications in gasproduction and true digestibility in in vitro techniques. British Journal of Nutrition73,897–913.

Musa AE, Gasmelseed GA. 2012. Characterization of Lawsonia inermis (Henna) as vegetable tanning material. Journal of Forest Products & Industries 1, 35-40.

Sadasivam S, Manickam A. 2008. Biochemical methods, 3 ed. New Age International Pvt. Ltd, New Delhi.

Sarker SD, Latif Z, Gray AI. 2005. Natural products isolation, Springer.

Soares LAL, Maia A, Oliveira AL, Petrovick, PR, Ortega GG.2006. Avaliação de Complexos Formadospor Catequinae Macromoléculas. Acta Farmacéutica Bonaerense 25, 10-6.

Tonon R, Grosso C, Hubinger M. 2011. Influence of emulsion composition and inlet air temperature on the microencapsulation of flaxseed oil by spray drying. Food Research International 44,282-289.

Ukoha PO, Cemaluk EAC, Nnamdi OL, Madus EP. 2011. Tannins and other phytochemical of the Samanaea saman pods and their antimicrobial activities. African Journal of Pure and Applied Chemistry. 5, 237-244.

Verza SG, Kreinecker MT, Reis V, Henriques AT, Ortega GG. 2007. Evaluation of analytical variables of the Folin-Ciocalteu method for the quantitation of the total tannins content using a Psidium guajava L. leaves aqueous extract as a model. Química Nova 30, 815-820.

Related Articles

Using geoinformatics to reduce cultural extinction, language and cultural identity crises, diasppearing cultural landscapes and cultural heritage loss among the Igbos of Southeast, Nigeria

B. N. Igbokwe, N. F. Nwulu*, F. U. Nnamani, C. I. Ahumaraeze, U. A. Agwuocha, M. A. C. Osuji, K. U. Chukwu, N. Emeka-Duru, G. O. Mbarah, C. F. Obumneke, F. O. Egwim, C. F. Nlemchukwu, C. M. Okwara, C. R. Ayozie, J. U. Chikaire, Int. J. Biosci. 28(6), 141-154, June 2026.

Eco-linguistics strategies for promoting rural literacy among farming households and farm families in Southeast, Nigeria

N. F. Nwulu*, F. U. Nnamani, C. R. Ayozie, C. F. Obumneke, B. N. Igbokwe, C. I. Ahumaraeze, U. A. Agwuocha, M. A. C. Osuji, F. O. Egwim, K. U. Chukwu, G. O. Mbarah, N. Emeka-Duru, C. F. Nlemchukwu, C. M. Okwara, J. U. Chikaire, Int. J. Biosci. 28(6), 128-140, June 2026.

Evaluation of the effectiveness of some plant extracts against cowpea charcoal rot

Manan Djamila Baikoro*, Dianyagou Paul Ouali, Wendoléan Romain Soalla, Pawindé Elisabeth Zida, Bouma James Neya, Marie Laure Guissou, Int. J. Biosci. 28(6), 118-127, June 2026.

Physicochemical profiling and quality evaluation of commercial mustard oils brands in Bangladesh: Indicators of stability and consumer safety

Umme Salma Nahida, Md. Al-Amin Mia, Afroza Bashar*, Tauhida Tasnim, Marjina Akter, Int. J. Biosci. 28(6), 108-117, June 2026.

Optimizing soybean (Glycine max L. Merr.) performance through rhizobial inoculation and planting density in Kétou, Benin

Mahougnon Charlotte Carmelle Zoundji*, Ibouraïman Balogoun, Pascal Gbenou, Tobi Moriaque Akplo, Carlosse Djeho, Félix Kouélo Alladassi, Int. J. Biosci. 28(6), 99-107, June 2026.

Genetic admixture and the philosophy of diplomacy in central Asia: Evidence from intercultural dialogue, governance and genomic data

Shafee Ur Rehman, Waqar Ahmed Khan, Iqra Jamil, Muhammad Abdullah, Int. J. Biosci. 28(6), 89-98, June 2026.