Rapid biosynthesis of highly stabilized nano silver by Andrographis paniculata leaf extract; A prioritized medicinal plant and its germicidal activity
Paper Details
Rapid biosynthesis of highly stabilized nano silver by Andrographis paniculata leaf extract; A prioritized medicinal plant and its germicidal activity
Abstract
The present research explains a rapid and environmental friendly route for the synthesis of highly stabilized silver nanoparticles using the Andrographis paniculata leaf extract. The biosynthesized silver nanoparticles were characterized by using UV-visible spectrophotometer, Fourier Transform Infrared spectroscopy, X-ray diffraction, and Scanning electron microscope. The UV- visible spectrum exhibited surface Plasmon resonance band at 421 nm signifies the presence of silver nanoparticles in the reaction mixture. The XRD technique revealed the synthesized nanoparticles were crystalline in nature as well as possess face centered cubic geometry. FTIR studies were carried out to investigate the functional groups responsible for the silver nanoparticle reduction in the range 4000 – 400cm-1. EDAX analysis displayed the elemental composition in the sample. SEM analysis confirmed the synthesized particles are polydispersed in nature. The inhibition zone gradually increased with increase in silver nanoparticle concentration. Further the silver nanoparticle exhibited an effective germicidal activity against both Gram positive and Gram negative organisms by using disc diffusion method. The in-vitro antimicrobial assay demonstrated the results of maximum inhibition zone with the (36±0.334) in Lactobacillus sp and minimum zone with (15±0.318) in Streptococcus sp. Because of the potent antimicrobial activity it might be concluded that silver nanoparticles were efficiently utilized as an effective antibacterial compounds.
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M. Revathy, C. Aswathy, S. Amutha, E. Amutha, T. Madhumitha, E. Pushpalakshmi, R. Venkateshwari, S. Rajaduraipandian, G. Annadurai (2023), Rapid biosynthesis of highly stabilized nano silver by Andrographis paniculata leaf extract; A prioritized medicinal plant and its germicidal activity; JBES, V22, N5, May, P17-24
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