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Repair of fractures in the bone of caudal fin for Xiphophorus helleri fish under the influence of hydrocortisone hormone

Ali A.A. Al-Ali, Muneerah A. Ibrahim

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Int. J. Biosci.15(4), 115-126, October 2019

DOI: http://dx.doi.org/10.12692/ijb/15.4.115-126


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The manufactured Glucocorticoid hormones (GC) are regarded as one of the most important drugs which used in treatment of many pathological conditions. The use of (GC) causes risk increasing of fractures at all age levels, as it affects the process of repairing fractures. The current experiment was designed by using male fish Xiphophorus helleri (24 male) which were divided in to two groups. The first group was a control group while the second group was treated. The second group were treated with Hydrocortisone with 2.5 mg/l concentration. After 8 days of treatment, the fractures were done in the caudal fins of both groups. This was done with anatomical microscope by a blade in different places of fin rays of caudal fin. Samples were saved for both groups by formalin stabilizer concentration 10% at different time intervals (0, 2, 5, and 14) day, starting at zero hour by three replicates for each stage. The samples were examined by a SEM and tissues pieces was prepared by using paraffin waxes. The results showed after two days a repair occurs to the soft tissue around the fracture position in both group.  As the days progressed, the amount of osteoid increased which caused fracture area fusion, while treated group samples were still free of any bony material till the last day of the experiment. The present study suggested the Hydrocortisone hormone has a positive effect on repairing the soft tissues and negative effects on repairing hard tissues.


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Repair of fractures in the bone of caudal fin for Xiphophorus helleri fish under the influence of hydrocortisone hormone

Al-saimary AS. 2018. Effect of hydrocortisone hormone on the bone       tissue differentiation and some histological and physiological changes in female’s mice Mus musculus L. P.H.D. thesis, Basra University/ Collage of Education for Pure Science, p 217.

Aslan M, Simsek G, Yildirim U. 2005. Effect of short-term treatment with systemic prednisone on bone healing: an experimental study in rats. Dental Traumatology 21,222-225. http://dx.doi.org/10.1111/j.1600-9657.2005.00300.x

Bancroft JD, Gamble M. 2008. Theory and Practice of Histological Techniques. 6th ed. Churchill Livingstone 692p.

Bechara IJ, Joazeiro PP, Marí-Beffa M, Becerra J, Montes GS. 2000.  Collagen-affecting drugs impair regeneration of teleost tail fins. Journal of Submicroscopic Cytology and Pathology 32(2), 273-280.

Bourcier T, Forgez P, Borderie V, Scbeer S, Rostène W, Larocbe L. 2002. Regulation of human corneal epithelial cell proliferation and apoptosis by Dexamethasone. Investigative Ophthalmology & Visual Science 41(13), 4133-4141.

Bouvard B, Legrand E, Audran M, Chappard D. 2010. Glucocorticoid-Induced Osteoporosis: A Review. Clinical Reviews in Bone and Mineral Metabolism 8, 15-26. http://dx.doi.org/10.1007/s12018-009-9051-9

Canalis E, Delany AM. 2002. Mechanisms of glucocorticoid action in bone. Annals of the New York Academy of Sciences 966, 73-81. http://dx.doi.org/10.1111/j.17496632.2002.tb04204.x

Canalis E, Mazziotti G, Giustina A, Bilezikian JP. 2007. Glucocorticoid-induced osteoporosis: pathophysiology and therapy. Osteoporosis International 18(10), 1319-1328. http://dx.doi.org/10.1007/s00198-007-0394-0

Dovio A, Perazzolo L, Saba L, Termine A, Capobianco M, Bertolotto A, Angeli A. 2006. High-dose glucocorticoids increase serum levels of soluble IL-6 receptor alpha and its ratio to soluble gp130: an additional mechanism for early increased bone resorption. European Journal Endocrinology 154(5), 745-751. https://doi.org/10.1530/eje.1.02147

Hachemi Y, Rapp A E, Picke A K, Weidinger G, Ignatius A, Tuckermann J. 2018. Molecular mechanisms of glucocorticoids on skeleton and bone regeneration after fracture. Journal of Molecular Endocrinology 61, 75-90. http://dx.doi.org/10.1530/JME-18-0024

Høgevold HE, Grøgaard B, Reikerås O. 1992. Effects of short- term treatment with corticosteroids and indomethacin on bone healing. A mechanical study of osteotomies in rats. Acta Orthopaedica Scandinavica 63(6), 607-611. http://dx.doi.org/10.1080/17453679209169718

Humason GL. 1972. Animal tissue techniques. 3rd ed. W. h. Freeman and Company, son fran- 614 p.

Kanis JA, Johansson H, Oden A, Johnell O, de Laet C, Melton lll LJ, Tenenhouse A, Reeve J, Silman AJ, Pols HA, Eisman JA, McCloskey EV, Mellstrom D. 2004. A meta-analysis of prior corticosteroid use and fracture risk. Journal of Bone and Mineral Reviews 19, 893-899. http://dx.doi.org/10.1359/JBMR.040134.

 Kim HJ, Zhao H, Kitaura H, Bhattacharyya S, Brewer JA, Muglia LJ, Ross FP, Teitelbaum SL. 2006. Glucocorticoids suppress bone formation via the osteoclast. Journal of Clinical. Investigation 116(8), 2152-2160. http://dx.doi.org/10.1172/JCI28084.

Kim HJ, Zhao H, Kitaura H, Bhattacharyya S, Brewer JA, Muglia LJ, Ross FP, Teitelbaum SL. 2007. Glucocorticoids and the osteoclast. Annals of the New York Academy of Sciences 1116, 335-339. http://dx.doi.org/10.1196/annals.1402.057

Lee Y, Hami D, De Val S, Kagermeier-Schenk B, Wills AA, Black BL, Weidinger G, Poss KD. 2009. Maintenance of blastemal proliferation by functionally diverse epidermis in regenerating zebrafish fins. Developmental Biology 331, 270-280. http://dx.doi.org/10.1016/j.ydbio.2009.05.545

Li J, Wang X, Zhou C, Liu L, Wu Y, Wang D, Jiang H. 2012. Perioperative glucocorticosteroid treatment delays early healing of a mandible wound by inhibiting osteogenic differentiation. Injury 43, 12841289. https://doi.org/10.1016/j.injury.2012.04.014)

Liu Y, Akhter MP, Gao X, Wang Xiao-yan, Wang Xiao-bei Zhao G, Wei X, Wu H, Chen H, Wang D, Cui L. 2018. Glucocorticoid- induced delayed fracture healing and impaired bone biomechanical properties in mice. Clinical Interventions in Aging 13, 1465-1474. https://doi.org/10.2147/CIA.S167431

Liu Y, Cui Y, Chen Y, Gao X, Su Y, Cui L. 2015. Effects of dexamethasone, celecoxib and methotrexate on the histology and metabolism of bone tissue in healthy Sprague Dawley rats. Clinical Interventions in Aging 10, 1245-1253. https://doi.org/10.2147/CIA.S85225

Liu Y, Cui Y, Zhang X, Gao X, Su Y, Xu B, Wu T, Chen W, Cui L. 2016. Effects of salvianolate on bone metabolism in glucocorticoid-treated lupus-prone B6.MRL-Fas(lpr)/J mice. Drug Design Development and Therapy 10, 2535-2546. http://dx.doi.org/10.2147/DDDT.S110125

Mathew LK, Sengupta S, Kawakami A, Andreasen EA, Lohr CV, Loynes CA, Renshaw SA, Peterson RT, Tanguay RL. 2007. Unraveling tissue regeneration pathways using chemical genetics. Journal of Biological Chemistry 282, 35202-35210. http://dx.doi.org/10.1074/jbc.M706640200

Mazloumi N, Amiri BM, Nematollahi M, Rafieem G. 2015. Hydrocortisone treatment may enhance survival and stocking of Beluga sturgeon (Huso huso Linnaeus, 1758) in estuaries of the Caspian Sea. International Journal of Aquatic Biology 3(3), 129-134.

McDonough AK, Curtis JR, Saag KG. 2008. The epidemiology of glucocorticoid- associated adverse events. Current Opinion in Rheumatology 20, 131-137. http://dx.doi.org/10.1097/BOR.0b013e3282f51031

Oishi Y, Fu ZW, Ohnuki Y, Kato H, Noguchi T. 2002. Molecular basis of the alteration in skin collagen metabolism in response to in vivo dexamethasone treatment: effects on the synthesis of collagen type I and III, collagenase, and tissue inhibitors of metalloproteinases. The British Journal of Dermatology 147(5), 859-868. http://dx.doi.org/10.1046/j.1365-2133.2002.04949.x

Oppedal D, Goldsmith MI. 2010. A chemical screen to identify novel inhibitors of fin regeneration in zebrafish. Zebrafish 7, 53-60. http://dx.doi.org/10.1089/zeb.2009.0633

Rubin J, Biskobing DM, Jadhav L, Fan D, Nanes MS, Perkins S, Fan X. 1998. Dexamethasone promotes expression of membrane – bound macrophage colony-stimulating factor in murine osteoblast-like cells. Endocrinology 139(3), 1006-1012. https://doi.org/10.1210/endo.139.3.5778

Sandberg OH, Aspenberg P. 2015. Glucocorticoids inhibit shaft fracture healing but not metaphyseal bone regeneration under stable mechanical conditions. Bone and joint Research 4, 170-175. http://dx.doi.org/10.1302/2046-3758.410.2000414

Sawin PD, Dickman CA, Crawford NR, Melton MS, Bichard WD Sonntag VK. 2001. The effects of dexamethasone on bone fusion in an experimental model of posterolateral lumbar spinal arthrodesis. Journal of Neurosurgery 94(1Supp1), 76-81. http://dx.doi.org/10.3171/spi.2001.94.1.0076

Tolar J, Teitelbaum SL, Orchard PJ. 2004. Osteopetrosis. The New England Journal of Medicine 351, 2839-2849.

Van Staa TP, Leufkens HG, Abenhaim L, Begaud B, Zhang B, Cooper C. 2000. Use of oral corticosteroids in the United Kingdom. Monthly Journal of The Association of physicians 93, 105-111. http://dx.doi.org/10.1093/qjmed/93.2.105

Waters RV, Gamradt SC, Asnis P, Vickery BH, Avnur Z, Hill E, Bostrom M. 2000. Systemic corticosteroids inhibit bone healing in a rabbit ulnar osteotomy model. Acta Orthopaedica Scandinavica 71(3), 316-321. http://dx.doi.org/10.1080/000164700317411951

Weinstein RS. 2011. Clinical practice. Glucocorticoid-induced bone disease. The New England Journal of Medicine 365(1), 62-70. http://dx.doi.org/10.1056/NEJMcp1012926

Weinstein RS, Jilka RL, Parfitt AM, Manolagas SC. 1998. Inhibition of osteoblast genesis and promotion of apoptosis of osteoblasts and osteocytes by glucocorticoids. Potential mechanisms of their deleterious effects on bone. The Journal of Clinical Investigation 102(2), 274-282. http://dx.doi.org/10.1172/JCI2799


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