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Repair of fractures in the bone of caudal fin for Xiphophorus helleri fish under the influence of hydrocortisone hormone

Research Paper | October 1, 2019

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Ali A.A. Al-Ali, Muneerah A. Ibrahim

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Int. J. Biosci.15( 4), 115-126, October 2019

DOI: http://dx.doi.org/10.12692/ijb/15.4.115-126


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The manufactured Glucocorticoid hormones (GC) are regarded as one of the most important drugs which used in treatment of many pathological conditions. The use of (GC) causes risk increasing of fractures at all age levels, as it affects the process of repairing fractures. The current experiment was designed by using male fish Xiphophorus helleri (24 male) which were divided in to two groups. The first group was a control group while the second group was treated. The second group were treated with Hydrocortisone with 2.5 mg/l concentration. After 8 days of treatment, the fractures were done in the caudal fins of both groups. This was done with anatomical microscope by a blade in different places of fin rays of caudal fin. Samples were saved for both groups by formalin stabilizer concentration 10% at different time intervals (0, 2, 5, and 14) day, starting at zero hour by three replicates for each stage. The samples were examined by a SEM and tissues pieces was prepared by using paraffin waxes. The results showed after two days a repair occurs to the soft tissue around the fracture position in both group.  As the days progressed, the amount of osteoid increased which caused fracture area fusion, while treated group samples were still free of any bony material till the last day of the experiment. The present study suggested the Hydrocortisone hormone has a positive effect on repairing the soft tissues and negative effects on repairing hard tissues.


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Repair of fractures in the bone of caudal fin for Xiphophorus helleri fish under the influence of hydrocortisone hormone

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